2014
DOI: 10.1016/j.cell.2014.04.028
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Quantitative Temporal Viromics: An Approach to Investigate Host-Pathogen Interaction

Abstract: SummaryA systematic quantitative analysis of temporal changes in host and viral proteins throughout the course of a productive infection could provide dynamic insights into virus-host interaction. We developed a proteomic technique called “quantitative temporal viromics” (QTV), which employs multiplexed tandem-mass-tag-based mass spectrometry. Human cytomegalovirus (HCMV) is not only an important pathogen but a paradigm of viral immune evasion. QTV detailed how HCMV orchestrates the expression of >8,000 cellul… Show more

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Cited by 396 publications
(603 citation statements)
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References 205 publications
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“…This is in accordance with a recent proteomics study of HCMV-infected cells, indicating that pUL77 and pUL93 are late proteins expressed simultaneously (57). In our immunoblotting experiments, pUL93 was seen as a major band migrating with a molecular mass of 68 kDa and as a minor form of 48 kDa.…”
Section: Discussionsupporting
confidence: 93%
“…This is in accordance with a recent proteomics study of HCMV-infected cells, indicating that pUL77 and pUL93 are late proteins expressed simultaneously (57). In our immunoblotting experiments, pUL93 was seen as a major band migrating with a molecular mass of 68 kDa and as a minor form of 48 kDa.…”
Section: Discussionsupporting
confidence: 93%
“…to be regulated efficiently during viral infection by a mechanism analogous to viral downregulation of MHC class I or NKG2D ligands, implying that immune evasion of BTN-mediated activation of γδ T cells is a requirement for viral infection (76)(77)(78).…”
Section: Mr1-restricted Presentation Of Bacterial Metabolites To Maitmentioning
confidence: 99%
“…Furthermore, several of the targets of siRNA in our screening data were not found in a proteomic study of HFFs infected with HCMV (27). Therefore, analysis of siRNA off-target binding alone may not be sufficient to exclude siRNAs from analysis.…”
Section: Discussionmentioning
confidence: 90%
“…Indeed, analysis of siRNA screening data can include a comparison of screening hits with gene expression profiles to identify false positives (7). Further analysis of siRNA screening data for HCMV-infected cells should benefit from a comparison of siRNA screening hits with proteins known to be expressed in HCMVinfected cells (27), as we have performed here. It remains unknown what RNAs are targeted by the siRNAs that we judged to be false positives in our screening data.…”
Section: Discussionmentioning
confidence: 99%