Quantitative thin layer chromatography for the determination of medroxyprogesterone acetate using a smartphone and open-source image analysis

A novel smartphone HPTLC assaying platform versus traditional densitometric method for simultaneous quantification of alfuzosin and solifenacin in their dosage forms as well as monitoring content uniformity and drug residues on the manufacturing equipment

“…c T = W 0.05 /2f, where W 0.05 is peak width at 5% of height and f is distance between peak maximum and leading edge. d N = 16(z/ w) 2 , where z is the migration length of the spot and w is the spot width. Optimization of image acquisition and analysis.…”

“…To overcome this limitation, several TLC staining reagents were tried to achieve stable, intensely coloured spots for both studied drugs without fading with time. 2,33 Those reagents included iodine crystals, Dragendorff's reagent, ninhydrin, anisaldehyde and phosphotungstic acid. Although the literature reported the utilization of iodine crystals as a universal visualizing agent producing brown spots, 6,45 the intensity of the coloured spots was found to be highly variable, very sensitive to time and rapidly diminishing.…”

“…9,10 ImageJ is wellestablished soware for the quantication of electrophoretic gels and has been recently integrated with TLC imaging analysis. 2,4,11 It is characterized by a user-friendly interface that allows straightforward application without requiring sophisticated manipulation steps or highly trained personnel. In comparison to other mobile applications for image analysis, ImageJ is superior in dealing with different image formats regardless of the smartphone operating system.…”

“…Digital image analysis is then employed to gauge the spot colour intensity of calibration standards so that the concentration of unknown samples can be found via the obtained regression equation. Such an approach has been broadly applied and has proven its validity for the determination of pharmaceutical drugs 2,3 and medicinal plants 4,5 as well as monitoring counterfeit drugs. 6,7 The digital image analysis is executed through either various chemometric manipulations or commercially available mobile applications and computer programs.…”

Development of a rapid method for the quantification of methamidophos and chlorpyrifos by Thin Layer Chromatography in soils using image processing

“…A esto se suma la aplicación de métodos de densitometría para el análisis de las manchas en las placas cromatográficas [6]. Sin embargo, esto también puede traer consigo costos adicionales, frente a esto, hoy en día se han desarrollado diversas alternativas para determinar diversos compuestos orgánicos por CCF mediante análisis o procesamiento de imágenes [7], [8]. Una alternativa que elimina costos adicionales es el software "Image J" que presenta aplicaciones en el tratamiento o procesamiento de imágenes en áreas de la salud [9]- [13] y fitoquímica [14], por ello en la presente investigación se buscó desarrollar un método rápido para la cuantificación de metamidofos y clorpirifós por cromatografía en capa fina en suelos mediante procesamiento de imágenes utilizando el software "Image J…”