Quantitative thin-layer chromatography

Light absorption spectrometry

“…Quantitative reflectance photometric measurements of thin layer chromatograms for the determination of amino acids, amino acid derivatives, and nucleic acid derivatives have been investigated (886). The spectrophotometric determination of small disks from paper chromatograms by the use of a special adapter has been described (300).…”

Fluorometric analysis

“…The fluorometric quantitation of thinlayer chromatogram spots, as applied to pharmacology and other fields, has been reviewed by Messerschmidt (601,602) and the precision of this technique has been found to be somewhat poorer than quantitation by reflectance photometry, in a study on amino acid and nucleic acid analyses on ninhydrin-sprayed silica gel plates (1073). Reviews have appeared on fluorescence techniques in studying enzyme kinetics (1018), amino acid metabolism (658), drug interactions with biological systems (165), and microscopy in clinical chemistry (1025).…”

Diffuse Reflectance Spectroscopy

“…The preparation and chromatographic separation have been discussed by Pataki. 270 Pataki 253 and Ziircheret al 286 have reported on the qualitative and quantitative evaluation of DNP and PTH amino acids by diffuse reflectance spectroscopy after separation of the derivatives on thin-layer plates. The reflectance spectra served conveniently for the identification of the amino acids; further differentiation of the spectra could be achieved by subsequent treatment of the spots with HC1 or NaOH spray solutions.…”