“…However, studying fluid inclusions poses many difficulties for precise analysis because of the high mobility and evaporation involved. Therefore, non-destructive analytical methods, such as micro-Raman spectroscopy (Burke, 2001;Frezzotti et al, 2012), synchrotron-radiation X-ray fluorescence (SR-XRF) (Schmidt and Rickers, 2003;Tsuchiyama et al, 2009) and proton-induced X-ray emission (PIXE) (Kurosawa et al, 2003), have been developed for the chemical analysis of individual fluid inclusions in minerals. It is also necessary to observe the spatial distribution of different fluid inclusion populations within a mineral grain to establish their paragenetic succession relative to the mineral formation (i.e., primary, secondary and pseudo-secondary inclusions) (e.g., Takeuchi, 1975;Roedder, 1984).…”