Quantitative trait loci affecting intensity of violet flower colour in potato

Abstract

Anthocyanins occur in potato tuber skin and flesh, sprouts, leaves, stems and flowers. The goal of this study was to identify genomic regions and candidate gene alleles key for accumulation of anthocyanins in potato corolla in various quantities. QTL analyses were performed in two mapping populations segregating for flower colour intensity and candidate genes were identified on the basis of function and location (chalcone isomerase, chi; chalcone synthase, chs) or location (RNA-dependent RNA polymerase 1, RDR1). We detected three and four QTL affecting the violet flower colour intensity using the two mapping populations, respectively. In both populations a locus F, necessary for violet flower colour, segregated and we used different approaches to differentiate the qualitative effect of this locus and to detect the genetic factors affecting the quantitative flower colour intensity. The strongest QTL and the only one common for the two mapping populations was located on chromosome V. The role of all three candidate genes, chi, chs and RDR1, in control of flower colour intensity is supported to different extents by the performed genetic analyses. The most important QTL on chromosome V is most likely in the same position as the QTL for anthocyanin tuber flesh coloration described previously, which indicates that the natural variation in some biosynthetic and/or regulatory genes may influence anthocyanin levels in multiple tissues.

Characterization of the F Locus Responsible for Floral Anthocyanin Production in Potato

“…The 1:1 segregation pattern for flower color in the DRH F 1 segregating population combined with a presence/absence phenotype imply the flower color is due to segregation of a single regulatory gene. If there had been a continuum of color or a more complex segregation pattern, a biosynthetic gene might have been a more likely cause, as Śliwka et al (2017) hypothesized in their study on flower color intensity. Genetic mapping revealed a significant QTL on the distal end of chromosome 10 which, when combined with previous reports that this region harbors the requisite F locus for flower color ( van Eck et al 1993 ), provides further support for this assertion.…”

“…The 1:1 segregation pattern for flower color in the DRH F 1 segregating population combined with a presence/absence phenotype imply the flower color is due to segregation of a single regulatory gene. If there had been a continuum of color or a more complex segregation pattern, a biosynthetic gene might have been a more likely cause, as Sliwka et al (2017) hypothesized Figure 4 IGV image of sequencing coverage of the DRH F 1 bulk segregant and monoploid haplotypes sorted by flower color aligned to chr10:51,474,000..51,469,500 of DM PGSC v4.03 Pseudomolecules. (Note that DM is white-flowered.)…”

StCoExpNet: a global co-expression network analysis facilitates identifying genes underlying agronomic traits in potatoes

“…Finally, the synthesized anthocyanins will be transported to the vacuole by the MATE transporter (Gomez et al 2009 ). The genetic basis of the anthocyanin biosynthetic pathway has been studied in potatoes and identified several essential genes involved in this pathway (Jung et al 2009 ; Zhang et al 2009a ; Zhang et al 2009b ; Śliwka et al 2017 ; Laimbeer et al 2020 ). However, so far, only a few studies have been conducted to investigate the transcriptional dynamics of the identified vital genes between different colored phenotypes (Laimbeer et al 2020 ; Riveros-Loaiza et al 2022 ).…”

A global co-expression network analysis facilitates identifying genes underlying agronomic traits in potatoes

“…Finally, the synthesized anthocyanins will be transported to the vacuole by the MATE transporter(Gomez et al, 2009). The genetic basis of the anthocyanin biosynthetic pathway has been studied in potatoes and identi ed several essential genes involved in this pathway(Jung et al, 2009;Zhang et al, 2009a;Zhang et al, 2009b;Śliwka et al, 2017;Laimbeer et al, 2020). However, so far, only a few studies have been conducted to investigate the transcriptional dynamics of the identi ed vital genes between different coloured phenotypes(Laimbeer et al, 2020;Riveros-Loaiza et al, 2022).…”