Quantitative Trait Loci Analysis for Peripheral Blood Parameters in a (BALB/cW * C57BL/6J-Mpl hlb219/J) F2 Mice

Strzałkowska, Adriana; Unrug-Bielawska, Katarzyna; Bluszcz, A; Sandowska-Markiewicz, Zuzanna; Karaszewska, J.; Pyśniak, Kazimiera; Gajewska, Marta; Wirth-Dzieciolowska, E.

doi:10.1538/expanim.60.405

Cited by 2 publications

(2 citation statements)

References 41 publications

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“…The higher levels of DMT1 in the liver resulting from the loss of Ndfip2 actually place these mice at a competitive advantage over WT mice in that there is more available iron to be released (as indicated by the reduction in liver iron levels) to compensate for the reduction in iron taken up from the diet. The stronger phenotype in the females may reflect a possible increase in sensitivity to iron levels suggested by hematological data, both in our study ( Table 1 ), and in work previously reported 25 . Earlier studies have also reported sex-specific changes in iron parameters in mice on both iron-deficient and iron overload diets 26 27 .…”

Section: Discussionsupporting

confidence: 74%

Ndfip2 is a potential regulator of the iron transporter DMT1 in the liver

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Gembus

Mackenzie

et al. 2016

Sci Rep

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The regulation of divalent metal ion transporter DMT1, the primary non-heme iron importer in mammals, is critical for maintaining iron homeostasis. Previously we identified ubiquitin-dependent regulation of DMT1 involving the Nedd4 family of ubiquitin ligases and the Ndfip1 and Ndfip2 adaptors. We also established the in vivo function of Ndfip1 in the regulation of DMT1 in the duodenum of mice. Here we have studied the function of Ndfip2 using Ndfip2-deficient mice. The DMT1 protein levels in the duodenum were comparable in wild type and Ndfip2−/− mice, as was the transport activity of isolated enterocytes. A complete blood examination showed no significant differences between wild type and Ndfip2−/− mice in any of the hematological parameters measured. However, when fed a low iron diet, female Ndfip2−/− mice showed a decrease in liver iron content, although they maintained normal serum iron levels and transferrin saturation, compared to wild type female mice that showed a reduction in serum iron and transferrin saturation. Ndfip2−/− female mice also showed an increase in DMT1 expression in the liver, with no change in male mice. We suggest that Ndfip2 controls DMT1 in the liver with female mice showing a greater response to altered dietary iron than the male mice.

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Section: Discussionsupporting

confidence: 74%

Ndfip2 is a potential regulator of the iron transporter DMT1 in the liver

Foot

Gembus

Mackenzie

et al. 2016

Sci Rep

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“…[63][64][65][66][67] Galarneau and colleagues performed one of the first studies showing that rare variants in genes implicated from GWAS studies can help identify causal genes in these loci for hematologic traits. 68 By sequencing candidate genes near loci implicated in fetal hemoglobin (HbF) level variation from GWAS, they were able to show that rare variants in MYB were associated with HbF levels.…”

Section: Genetic Basis Of Complex Traitsmentioning

confidence: 99%

Applications of high-throughput DNA sequencing to benign hematology

Sankaran

Gallagher

2013

Blood

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The development of novel technologies for high-throughput DNA sequencing is having a major impact on our ability to measure and define normal and pathologic variation in humans. This review discusses advances in DNA sequencing that have been applied to benign hematologic disorders, including those affecting the red blood cell, the neutrophil, and other white blood cell lineages. Relevant examples of how these approaches have been used for disease diagnosis, gene discovery, and studying complex traits are provided. High-throughput DNA sequencing technology holds significant promise for impacting clinical care. This includes development of improved disease detection and diagnosis, better understanding of disease progression and stratification of risk of disease-specific complications, and development of improved therapeutic strategies, particularly patient-specific pharmacogenomics-based therapy, with monitoring of therapy by genomic biomarkers.

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Quantitative Trait Loci Analysis for Peripheral Blood Parameters in a (BALB/cW * C57BL/6J-Mpl hlb219/J) F2 Mice

Cited by 2 publications

References 41 publications

Ndfip2 is a potential regulator of the iron transporter DMT1 in the liver

Ndfip2 is a potential regulator of the iron transporter DMT1 in the liver

Applications of high-throughput DNA sequencing to benign hematology

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