Quantitative trait loci associated with oligosaccharide and sucrose contents in soybean (Glycine max L.)

Kim, Hyeun-Kyeung; Kang, Sung-Taeg; Cho, Jun-Hyeun; Choung, Myoung-Gun; Suh, Duck-Yong

doi:10.1007/bf03030569

Cited by 53 publications

(69 citation statements)

References 21 publications

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“…However, the genotype and location effects were greater than other sources of variation when comparing variance components. The heritability of sucrose in this study (0.74) was similar to that reported by Maughan et al (2000) and Kim et al (2005). This was also supported by the fact that lines in the mapping population ranked similarly across locations.…”

Section: Discussionsupporting

confidence: 90%

“…In comparison with the reference 'Williams 82' physical map, these two QTL regions were within a similar proximity, with sucrose QTL in the F 3derived population being narrowed down to a smaller region in the SNP analysis (Table 8). In other studies, sucrose QTL were mapped by Kim et al (2005Kim et al ( , 2006 using data from only one location. In a previous study, a sucrose QTL flanked by Satt287 (at 1,631,658-1,631,885 bp of Gm16 in the Williams 82 physical map) and Sct_065 (at 10,498,889-10,499,048 bp of Gm 16, Williams 82) on chromosome 16 was identified by Kim et al (2006); this previously reported QTL was distantly located upstream from the QTL identified in this study.…”

Section: Discussionmentioning

confidence: 99%

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Identification of Quantitative Trait Loci for Sucrose Content in Soybean Seed

et al. 2014

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RESEARCHS oybean [Glycine max (L.) Merr.] is a major source of world protein meal and vegetable oil (Soy Stats, 2012). Because of its nutritional and health benefits, soybean is also processed into a variety of fermented and non-fermented food products. Fermented food products include natto, soy sauce, and miso; non-fermented soyfood products include tofu, kori-tofu, yuba, nimame, soymilk, edamame, bean sprouts, and kinako.Soybean seed contains, on average, 40% protein, 20% oil, 35% carbohydrates and 5% ash (Krober and Cartter, 1962); up to 47% of the carbohydrates are soluble sugars (Hymowitz and Collins, 1974). The major soluble sugars are glucose, fructose, disaccharide sucrose, and raffinose family oligosaccharides (RFOs) including raffinose and stachyose (Hymowitz and Collins, 1974;Liu, 1997). Among these soluble sugars, sucrose is the most abundant, which is 25 to 82 g kg -1 , followed by stachyose (14-41 g kg -1 ) and raffinose ABSTRACT Sucrose is a desirable sugar in soybean seed that affects the quality of soyfoods and feed; therefore, soybean cultivars with high sucrose would be valuable for soyfood and soybean meal markets. The objective of this study was to identify quantitative trait loci (QTL) associated with seed sucrose content using simple sequence repeat (SSr) and single nucleotide polymorphism (SNp) markers that can be used for indirect selection in breeding. A low sucrose line, MFS-553, was crossed with a high sucrose plant introduction, pI 243545, to develop an F 2 -derived QTL mapping population. A total of 626 SSr primers covering 20 soybean chromosomes were used to screen the parents and 209 SSr markers were polymorphic. The polymorphic SSr markers were used to genotype 220 F 2:3 lines. Subsequently, a total of 94 F 3:4 lines derived from the initial F 2:3 population were genotyped with 5361 SNp markers spanning 20 chromosomes, of which 2016 were polymorphic. Seed from the F 2:3 , F 3:5 , and F 3:6 lines were analyzed for sucrose using high performance liquid chromatography (HpLC). Following the linkage map construction, composite interval mapping (CIM) and multiple interval mapping (MIM) were performed to locate sucrose QTL. Three novel QTLs for seed sucrose were mapped to chromosomes 5, 9, and 16, accounting for 46, 10 and 8%, respectively, of the phenotypic variation for sucrose content. SSr and SNp markers linked to these QTLs can be used for marker assisted selection (MAS) in breeding soybean lines with the desired sugar profile.

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Section: Discussionsupporting

confidence: 90%

Section: Discussionmentioning

confidence: 99%

Identification of Quantitative Trait Loci for Sucrose Content in Soybean Seed

et al. 2014

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“…These explained a relatively low amount of phenotypic variation (< 10%) in sucrose content. However, they did not detect the QTL previously reported by Kim et al (2005), even though the high-sucrose parent used to derive populations was the same in both studies. Skoneczka et al (2009) identified a QTL on chromosome 6 (MLG C2), flanked by markers Sat213 and Satt643, which was responsible for low-stachyose and high-sucrose contents in PI 200508.…”

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confidence: 64%

“…No epistasis was observed among the identified sucrose QTL. Kim et al (2005) reported four QTL located on three chromosomes (2, 11 and 19) for sugar content using RIL populations derived from the cross of 'Keunolkong' 9 'Shinpaldalkong'. The major QTL, flanked by SSR markers Satt278 and Satt523, located on chromosome 19 explained 21.4% of the total phenotypic variation in sucrose content, while the other two QTL on chromosome 2 and 11 conferred minor effects, respectively.…”

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confidence: 98%

Quantitative trait loci analysis of soluble sugar contents in soybean

2014

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The soluble sugar content in soybean seeds, mainly sucrose, stachyose, raffinose and trace amounts of glucose and fructose, is important for the increasing global market demand for various soyfoods including tofu, soymilk, natto, bean sprouts and edamame due to their nutritional value and health benefits. The objective of this study was to conduct quantitative trait loci (QTL) analysis and identify molecular markers for soluble sugar content in soybean seeds for marker-assisted selection (MAS) in soybean breeding. The content of the five previously mentioned sugars were measured and associated QTLs were mapped based on a F 2 population that was derived from a cross between V97-3000 and V99-5089. Eleven QTLs were detected for the five sugar contents: one for glucose, three each for fructose and sucrose, and two each for raffinose and stachyose. However, only one QTL for sucrose, one QTL for raffinose, and two QTLs for stachyose were identified with LOD > 3.0 and R 2 > 10% from this research. The QTL on chromosome 11 [linkage group (LG) B1] was identified as associated with sucrose, raffinose and stachyose in the same region as previously reported for sucrose and stachyose. The SSR marker, Satt359, on the QTL B1 region had an significant association with sucrose (LOD = 5.192; R 2 = 0.134), raffinose (LOD = 3.95; R 2 = 0.104), and stachyose (LOD = 13.572; R 2 = 0.314); therefore it can be used to assist breeding selection for sucrose, raffinose and stachyose contents simultaneously.

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“…Kim et al. () identified two common QTL for oligosaccharide and sucrose in a recombinant inbred line (RIL) population. Utilizing F 2:3 population consisting 170 lines, Wang et al.…”

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confidence: 99%

Mapping quantitative trait loci controlling soybean seed starch content in an interspecific cross of ‘Williams 82’ (Glycine max) and ‘PI 366121’ (Glycine soja)

et al. 2017

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Seed starch content (SSC) greatly affects the taste, flavour and processing properties of soy foods. The objective in this study was to identify quantitative trait loci (QTL) for SSC in soybean. A total in 169 recombinant inbred lines (RILs) derived from a cross in ‘Williams 82’ and ‘PI 366121’ were grown for three consecutive years. The SSC of the RILs displayed continuous variation with transgressive segregation and hence amenable for QTL mapping. Nine significant QTL exhibiting 5.6–11.3% of the total phenotypic variation (PVE) were identified. The QTL qSTR06_2 showed highest PVE (9.1–11.3%) at LOD values of 4.25–5.39. No stable QTL over 3 years were identified, indicating strong environmental influence on SSC. The QTL qSTR11_1 and qSTR20_1 were found to colocalize with some of the previously reported QTL for sucrose content in soybean, implying the interrelationship between starch and sucrose biosynthesis. As the carbohydrate components may affect key constituents such as oil and protein in soybean seed, findings of the study may be useful in breeding soybeans with improved seed composition.

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Quantitative trait loci associated with oligosaccharide and sucrose contents in soybean (Glycine max L.)

Cited by 53 publications

References 21 publications

Identification of Quantitative Trait Loci for Sucrose Content in Soybean Seed

Identification of Quantitative Trait Loci for Sucrose Content in Soybean Seed

Quantitative trait loci analysis of soluble sugar contents in soybean

Mapping quantitative trait loci controlling soybean seed starch content in an interspecific cross of ‘Williams 82’ (Glycine max) and ‘PI 366121’ (Glycine soja)

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