Quantitative trait loci increasing acylsugars in tomato breeding lines and their impacts on silverleaf whiteflies

Leckie, Brian; Jong, Darlene M. De; Mutschler, Martha A.

doi:10.1007/s11032-012-9746-3

Cited by 66 publications

(119 citation statements)

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“…A QTL on chromosome 11 was identified that appears to colocalize with ASAT3 based on the markers used to define the QTL region (Leckie et al, 2012). Similar to our observation that total acylsugar levels decreased when plants harbored homozygous Sp-ASAT3 alleles, plants from multiple QTL mapping populations that were homozygous for this chromosome 11 region also showed low acylsucrose levels (Mutschler et al, 1996;Leckie et al, 2012). Interestingly, the QTL on chromosome 11 also had an effect on acylglucose production when combined with other introgressions (Leckie et al, 2013).…”

Section: Acylsugar Biosynthesis In a Broader Contextsupporting

confidence: 73%

“…A significant effort has been made to identify quantitative trait loci (QTL) affecting acylsugar production in tomato using crosses with S. pennellii LA0716 (Mutschler et al, 1996;Lawson et al, 1997), a wild tomato species that produces high levels of acylsugars, including copious acylglucoses (Fobes et al, 1985). A QTL on chromosome 11 was identified that appears to colocalize with ASAT3 based on the markers used to define the QTL region (Leckie et al, 2012). Similar to our observation that total acylsugar levels decreased when plants harbored homozygous Sp-ASAT3 alleles, plants from multiple QTL mapping populations that were homozygous for this chromosome 11 region also showed low acylsucrose levels (Mutschler et al, 1996;Leckie et al, 2012).…”

Section: Acylsugar Biosynthesis In a Broader Contextmentioning

confidence: 99%

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Functionally Divergent Alleles and Duplicated Loci Encoding an Acyltransferase Contribute to Acylsugar Metabolite Diversity in Solanum Trichomes

et al. 2015

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Section: Acylsugar Biosynthesis In a Broader Contextsupporting

confidence: 73%

Section: Acylsugar Biosynthesis In a Broader Contextmentioning

confidence: 99%

Functionally Divergent Alleles and Duplicated Loci Encoding an Acyltransferase Contribute to Acylsugar Metabolite Diversity in Solanum Trichomes

et al. 2015

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“…Fully dried leaf samples were washed with 3 ml of methanol. Acylsugar levels in the rinsate were measured as described by Leckie et al (2012), in which the Nelson reaction originally used to measure sugar was replaced by a modified peroxidase/glucose oxidase assay (Setter et al 2001). This modified assay provides improved results and eliminates use of arsenic compounds.…”

Section: Trichome and Acylsugar Analysesmentioning

confidence: 99%

Evaluation of wild tomato accessions (Solanum spp.) for resistance to two-spotted spider mite (Tetranychus urticae Koch) based on trichome type and acylsugar content

Rakha

Bouba

Srinivasan

et al. 2016

Genet Resour Crop Evol

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Tomato wild relatives are important sources of resistance to many pests of cultivated tomato [Solanum lycopersicum L. (syn. Lycopersicon esculentum Mill.)]. Eleven wild tomato accessions previously identified at AVRDC-The World Vegetable Center as resistant to Bemisia tabaci were evaluated for resistance to the two-spotted spider mite [Tetranychus urticae (Koch.)] based on egg numbers using the leaf disc and Tanglefoot no-choice bioassays, and damage scores in choice bioassays. Highest resistance based on choice and no-choice bioassays was identified in AVRDC S. galapagense accessions VI057400, VI045262, VI037869 and VI037239, and S. cheesmaniae accession VI037240, all of which are new sources of T. urticae resistance. In addition, S. pimpinellifolium accession VI030462 exhibited resistance only in the no-choice bioassay based on egg numbers. Resistance to T. urticae based on the number of eggs from the no-choice bioassays was positively correlated with density of type IV glandular trichomes and negatively correlated with densities of type V trichomes. All resistant accessions accumulated high levels of total acylsugars, which were positively associated with type IV trichomes. There was a significant negative relationship between acylsugar content and T. urticae egg numbers from the no-choice bioassays. There was high correlation between the results from the leaf disc test and the Tanglefoot nochoice bioassay. These findings support the possible presence of broad-based insect and mite resistance in accessions closely related to cultivated tomato.

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“…Nicotiana attenuata acylsucroses are at the center of a multitrophic defense interaction where they are metabolized to volatile fatty acids by Manduca sexta larvae, and these airborne products attract predatory ants (6). Protective properties against herbivores have made increasing total acylsugars or altering acyl chain types a target for breeding insect-resistant cultivated tomatoes (Solanum lycopersicum) (16)(17)(18). In addition, synthetic sucrose esters mimicking natural acylsugars have been applied as safe, biodegradable insecticidal compounds (19)(20)(21) and also have commercial value in the food, cosmetic, and pharmaceutical industries (22,23).…”

mentioning

confidence: 99%

In vitro reconstruction and analysis of evolutionary variation of the tomato acylsucrose metabolic network

Fan

Miller

Schilmiller

et al. 2015

Proc. Natl. Acad. Sci. U.S.A.

114

233

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Plant glandular secreting trichomes are epidermal protuberances that produce structurally diverse specialized metabolites, including medically important compounds. Trichomes of many plants in the nightshade family (Solanaceae) produce O-acylsugars, and in cultivated and wild tomatoes these are mixtures of aliphatic esters of sucrose and glucose of varying structures and quantities documented to contribute to insect defense. We characterized the first two enzymes of acylsucrose biosynthesis in the cultivated tomato Solanum lycopersicum. These are type I/IV trichome-expressed BAHD acyltransferases encoded by Solyc12g006330─or S. lycopersicum acylsucrose acyltransferase 1 (Sl-ASAT1)─and Solyc04g012020 (Sl-ASAT2). These enzymes were used-in concert with two previously identified BAHD acyltransferases-to reconstruct the entire cultivated tomato acylsucrose biosynthetic pathway in vitro using sucrose and acyl-CoA substrates.Comparative genomics and biochemical analysis of ASAT enzymes were combined with in vitro mutagenesis to identify amino acids that influence CoA ester substrate specificity and contribute to differences in types of acylsucroses that accumulate in cultivated and wild tomato species. This work demonstrates the feasibility of the metabolic engineering of these insecticidal metabolites in plants and microbes.Solanum | glandular trichomes | acylsugar | specialized metabolism | genotype to phenotype P lants are masters of metabolism, producing hundreds of thousands of small molecules known as specialized metabolites, which vary widely in structure, abundance, and physical and biological properties. These metabolites tend to be produced by enzymes that evolve faster than those that produce "central" metabolites such as amino acids, nucleotides, sugars, and cofactors (1-3), and the pathways and metabolic intermediates involved in biosynthesis of many specialized metabolites remain mysterious. Despite the growing availability of genomic DNA sequences, understanding the genetic and biochemical mechanisms that contribute to this phenotypic diversity and plasticity presents enduring and major challenges in plant biochemistry. It is of great interest to understand and manipulate the biosynthesis of these biologically active molecules.Specialized metabolites typically are produced in a cell-or tissue-specific manner and are generally limited in their taxonomic distribution. Glandular secreting trichomes provide an example of such a differentiated structure; these epidermal "hairs" produce a variety of metabolites of importance to humans, including aromatic flavor components (e.g., in hops for beer and Mediterranean herbs for cooking), psychoactive cannabinoids in Cannabis, and the antimalarial drug artemisinin in Artemisia annua (4, 5).Some trichome-produced metabolites have documented direct and indirect antiherbivore activities (4, 6-8). For example, acylsugars are a group of structurally related specialized metabolites produced in plants of the nightshade family-the Solanaceae (9, 10). Characterized examples ...

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Quantitative trait loci increasing acylsugars in tomato breeding lines and their impacts on silverleaf whiteflies

Cited by 66 publications

References 57 publications

Functionally Divergent Alleles and Duplicated Loci Encoding an Acyltransferase Contribute to Acylsugar Metabolite Diversity in Solanum Trichomes

Functionally Divergent Alleles and Duplicated Loci Encoding an Acyltransferase Contribute to Acylsugar Metabolite Diversity in Solanum Trichomes

Evaluation of wild tomato accessions (Solanum spp.) for resistance to two-spotted spider mite (Tetranychus urticae Koch) based on trichome type and acylsugar content

In vitro reconstruction and analysis of evolutionary variation of the tomato acylsucrose metabolic network

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