Quantitative trait loci of frost tolerance and physiologically related trait in faba bean (Vicia faba L.)

Abstract

In faba bean, field based winter-hardiness is a complex trait that is significantly correlated to frost tolerance. Frost tolerance could be used to indirectly select for faba bean winter-hardiness. The aim of this study was to identify putative QTL associated with frost tolerance and auxiliary traits and to quantify the efficiency of marker assisted selection. Thus, 101 recombinant inbred lines derived from the cross between two frost tolerant lines were tested for their hardened and unhardened frost tolerance and for their leaf fatty acid content in both treatments. Significant differences among the RIL were observed for all studied traits. For frost tolerance, five putative QTL were detected; three for unhardened frost tolerance that explained 40.7% (8.6% after crossvalidation, CV) of its genotypic variance and two for hardened frost tolerance that explained 21.8% (1.0% after CV). For fatty acid content, three QTL were detected for oleic acid content in unhardened leaves that explained 62.9% (40.6% after CV) of its genotypic variance. This fatty acid was significantly correlated with unhardened frost tolerance. The unbiased genotypic variance explained enabled to draw realistic prospects of MAS for frost tolerance. In this study, combined MAS was more efficient than classical phenotypic selection and was expected to be higher on larger populations at early generations. Moreover, favourable alleles inherited from the exotic line BPL 4628 could be introgressed to European winter-hardy beans for further improvement.

Screening and selection of faba beans (Vicia faba L.) for cold tolerance and comparison to wild relatives

“…Arbaoui and Link (2008) found that fatty acid composition was found to be related to frost tolerance in faba bean. Arbaoui et al (2008b) out lined that five putative quantitative trait loci (QTL) related with fatty acids in faba bean were detected for frost tolerance. Recently Link et al (2010) underlined that three QTLs were identified for frost tolerance (explaining 8.6% of the phenotypic variation).…”

Use of synteny to identify candidate genes underlying QTL controlling stomatal traits in faba bean (Vicia faba L.)

“…Molecular approaches in faba bean breeding have been mostly limited to biotic stresses and anti-nutritional compounds (reviewed in Torres et al 2010Torres et al , 2012, but amongst abiotic stresses, some progress has also been made in identifying QTLs in frost tolerance (Arbaoui et al 2008). Yield, its components, and flowering time (associated with drought avoidance) were fine mapped in faba bean (CruzIzquierdo et al 2012).…”

Genetic Adjustment to Changing Climates: faba bean

“…Faba beans shoots were found to contain much more glycinebetaine (125 μ moles per g dry weight) than Phaseolus vulgaris and Pisum sativum shoots where, moreover, trigonelline instead of glycinebetaine was found (Jones and Storey 1981). A number of carbohydrates have also been reported to show a protective impact (Dumont et al 2009), such as raffinose for petunia (Pennycooke et al 2003) and trehalose in Lotus japonicus (López et al 2006;Arbaoui et al 2008b).…”