Quantitative trait locus mapping for Verticillium wilt resistance in a backcross inbred line population of cotton (Gossypium hirsutum × Gossypium barbadense) based on RGA-AFLP analysis

Fang, Hui; Zhou, Huiping; Sanogo, Soum; Flynn, Robert; Percy, Richard G.; Hughs, S. E.; Ulloa, Mauricio; Jones, Don C.; Zhang, Jinfa

doi:10.1007/s10681-013-0965-4

Cited by 40 publications

(52 citation statements)

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“…These were linked to the common marker NAU3479. We could not correlate the other QTLs for VW resistance to those found in other studies due to the lack of common markers, although some QTLs were on the same chromosomes [15, 18, 20, 22, 23, 26, 27, 29, 36]. The more frequently informative molecular markers are used in the cotton community, the greater the likelihood that QTL conditioning VW resistance among various cotton germplasm lines could be tagged.…”

Section: Discussionmentioning

confidence: 82%

“…For example, Zhang et al detected QTLs for VW resistance based on a 392 SSR polymorphic loci linkage map covering 2895 cM or about 57.90 % of the cotton genome using the BIL population of SG 747 × Giza 75 [13]. Fang et al constructed a 292-marker genetic linkage map for the BIL population of SG 747 × Pima S-7 [15]. It covered 1226 cM or about 27 % of the cotton genome.…”

Section: Discussionmentioning

confidence: 99%

“…In the previous reports, most of these linkage maps used to identify QTLs associated with VW resistance had no more than 2000 SSR markers and covered less than 60 % of the cotton genome [2, 15, 16, 19, 22–24]. It is difficult to identify more QTLs for VW resistance from G. barbadense using the limited number of markers and low coverage of the whole cotton genome.…”

Section: Discussionmentioning

confidence: 99%

“…Recently, Said et al reported a meta QTL analyses and established CottonQTLdb database containing 2274 QTL for 66 different QTL trait types [34], including 139 QTLs of VW resistance from nine published papers [15, 16, 18, 19, 24–28]. We got the VW resistance QTL mapping information including their names and CI provided by Said et al [34].…”

Section: Methodsmentioning

confidence: 99%

“…Many genetic linkage maps have been constructed, and QTLs have been identified for VW resistance in cotton from interspecific populations of G. hirsutum × G. barbadense [2, 15–21] and from G. hirsutum intraspecific populations [22–27]. Some QTLs have also been detected by association mapping using a natural population, chromosome segment introgression lines and molecular mapping based on restriction-site associated DNA (RAD) sequencing technology using a RIL population [17, 28, 29].…”

Section: Introductionmentioning

confidence: 99%

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Quantitative trait loci analysis of Verticillium wilt resistance in interspecific backcross populations of Gossypium hirsutum × Gossypium barbadense

et al. 2016

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Background Verticillium wilt (VW) caused by Verticillium dahliae (Kleb) is one of the most destructive diseases of cotton. The identification of highly resistant QTLs or genes in the whole cotton genome is quite important for developing a VW-resistant variety and for further molecular design breeding.ResultsIn the present study, BC1F1, BC1S1, and BC2F1 populations derived from an interspecific backcross between the highly resistant line Hai1 (Gossypium barbadense L.) and the susceptible variety CCRI36 (G. hirsutum L.) as the recurrent parent were constructed. Quantitative trait loci (QTL) related to VW resistance were detected in the whole cotton genome using a high-density simple sequence repeat (SSR) genetic linkage map from the BC1F1 population, with 2292 loci covering 5115.16 centiMorgan (cM) of the cotton (AD) genome, and the data concerning VW resistance that were obtained from four dates of BC2F1 in the artificial disease nursery and one date of BC1S1 and BC2F1 in the field. A total of 48 QTLs for VW resistance were identified, and 37 of these QTLs had positive additive effects, which indicated that the G. barbadense alleles increased resistance to VW and decreased the disease index (DI) by about 2.2–10.7. These QTLs were located on 19 chromosomes, in which 33 in the A subgenome and 15 QTLs in the D subgenome. The 6 QTLs were found to be stable. The 6 QTLs were consistent with those identified previously, and another 42 were new, unreported QTLs, of which 31 QTLs were from G. barbadense. By meta-analysis, 17 QTL hotspot regions were identified and 10 of them were new, unreported hotspot regions. 29 QTLs in this paper were in 12 hotspot regions and were all from G. barbadense.ConclusionsThese stable or consensus QTL regions warrant further investigation to better understand the genetics and molecular mechanisms underlying VW resistance. This study provides useful information for further comparative analysis and marker-assisted selection in the breeding of disease-resistant cotton. It may also lay an important foundation for gene cloning and further molecular design breeding for the entire cotton genome.Electronic supplementary materialThe online version of this article (doi:10.1186/s12864-016-3128-x) contains supplementary material, which is available to authorized users.

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