Quantitative Trait Locus Mapping of Clubroot Resistance and Plasmodiophora brassicae Pathotype Banglim-Specific Marker Development in Brassica rapa

Choi, Su Ryun; Oh, Sang Heon; Chhapekar, Sushil Satish; Dhandapani, Vignesh; Lee, Chang Yeol; Rameneni, Jana Jeevan; Ma, Yinbo; Choi, Gyung Ja; Lee, Soo-Seong; Lim, Yong Pyo

doi:10.3390/ijms21114157

Cited by 10 publications

(10 citation statements)

References 68 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The responses of Chinese cabbage (B. rapa) lines to different P. brassicae pathotypes were compared on the basis of the disease index (DI). More specifically, the responses of CR Shinki and 94SK Chinese cabbage inbred lines to three well-known P. brassicae pathotypes (Race 4, Uiryeong, and Banglim) were analyzed [8,54] (Figure 1). In response to Race 4, the DI was significantly higher for the susceptible line (94SK) than for the resistant line (CR Shinki) (Figure 1A).…”

Section: Differences In the Responses Of Resistant (Cr Shinki) And Susceptible (94sk) B Rapa Inbred Lines To P Brassicae Pathotypesmentioning

confidence: 99%

“…Differences in the responses of CR Shinki (resistant) and 94SK (susceptible) Brassica rapa inbred lines to three Plasmodiophora brassicae pathotypes. Disease symptoms on B. rapa roots infected with P. brassicae pathotypes (A) Race 4 [8], (B) Uiryeong, and (C) Banglim [54]. Disease severity was assessed according to the degree of root morphological modifications using the following rating scale: 0 = no symptoms; 1 = very slight swelling, usually confined to the lateral roots; 2 = a few small, separate globular clubs on the lateral roots; 3 = moderate clubbing on the lateral roots; 4 = large clubs on the lateral roots and slight swelling of the main root; 5 = larger clubs on the main root than on the lateral roots; 6 = severe clubbing on all roots.…”

Section: Differences In the Responses Of Resistant (Cr Shinki) And Susceptible (94sk) B Rapa Inbred Lines To P Brassicae Pathotypesmentioning

confidence: 99%

See 1 more Smart Citation

MiR1885 Regulates Disease Tolerance Genes in Brassica rapa during Early Infection with Plasmodiophora brassicae

Pavlidis

Chhapekar

Rameneni

et al. 2021

IJMS

Self Cite

View full text Add to dashboard Cite

Clubroot caused by Plasmodiophora brassicae is a severe disease of cruciferous crops that decreases crop quality and productivity. Several clubroot resistance-related quantitative trait loci and candidate genes have been identified. However, the underlying regulatory mechanism, the interrelationships among genes, and how genes are regulated remain unexplored. MicroRNAs (miRNAs) are attracting attention as regulators of gene expression, including during biotic stress responses. The main objective of this study was to understand how miRNAs regulate clubroot resistance-related genes in P. brassicae-infected Brassica rapa. Two Brassica miRNAs, Bra-miR1885a and Bra-miR1885b, were revealed to target TIR-NBS genes. In non-infected plants, both miRNAs were expressed at low levels to maintain the balance between plant development and basal immunity. However, their expression levels increased in P. brassicae-infected plants. Both miRNAs down-regulated the expression of the TIR-NBS genes Bra019412 and Bra019410, which are located at a clubroot resistance-related quantitative trait locus. The Bra-miR1885-mediated down-regulation of both genes was detected for up to 15 days post-inoculation in the clubroot-resistant line CR Shinki and in the clubroot-susceptible line 94SK. A qRT-PCR analysis revealed Bra019412 expression was negatively regulated by miR1885. Both Bra019412 and Bra019410 were more highly expressed in CR Shinki than in 94SK; the same expression pattern was detected in multiple clubroot-resistant and clubroot-susceptible inbred lines. A 5′ rapid amplification of cDNA ends analysis confirmed the cleavage of Bra019412 by Bra-miR1885b. Thus, miR1885s potentially regulate TIR-NBS gene expression during P. brassicae infections of B. rapa.

show abstract

Section: Differences In the Responses Of Resistant (Cr Shinki) And Susceptible (94sk) B Rapa Inbred Lines To P Brassicae Pathotypesmentioning

confidence: 99%

Section: Differences In the Responses Of Resistant (Cr Shinki) And Susceptible (94sk) B Rapa Inbred Lines To P Brassicae Pathotypesmentioning

confidence: 99%

MiR1885 Regulates Disease Tolerance Genes in Brassica rapa during Early Infection with Plasmodiophora brassicae

Pavlidis

Chhapekar

Rameneni

et al. 2021

IJMS

Self Cite

View full text Add to dashboard Cite

show abstract

“…Three genes, Bra020951, Bra020974, and Bra020979, have been identified as candidates of the Rcr3 gene, and three genes related to immune-system-process (Bra020827, Bra020828, Bra020814) have been identified as candidates of the Rcr9 wa gene [53]. A clubroot resistance locus, covering PbBrA08 Banglim gene, was also detected on chromosome A08, where is near to Crr1, CRs, and Rcr9 [54]. These reports suggest that they are not allelic, thus chromosome A08 covering these genes has an R gene cluster.…”

Section: Isolation Of Resistance Genesmentioning

confidence: 99%

Breeding for Disease Resistance in Brassica Vegetables Using DNA Marker Selection

Akter¹,

Mehraj²,

Itabashi³

et al. 2021

Brassica Breeding and Biotechnology

View full text Add to dashboard Cite

The Brassica genus comprises of agro-economically important vegetables. Disease causes great yield loss of Brassica vegetables worldwide. Different traditional methods such as crop rotation and chemical control have limited effect on different diseases of Brassica vegetables and cannot completely eradicate the pathogens by these methods. Development of disease resistant cultivars is one of the most effective, ecofriendly, and cheapest measure to control Brassica diseases. With the development of genomics, molecular biology techniques, and biological methods, it is possible to discover and introduce resistance (R) genes to efficiently control the plant diseases caused by pathogens. Some R genes of major diseases such as Fusarium wilt and clubroot in Brassica vegetables have been already identified. Therefore, we will focus to review the Fusarium wilt and clubroot resistance in Brassica vegetables and the methodologies for identification, mapping, and pyramiding of R genes/quantitative trait loci (QTLs) to develop disease resistant cultivars. These techniques will be helpful for sustainable crop production and to maintain global food security and contribute to ensure protection of food supply in the Asian country as well as throughout the world.

show abstract

“…The key aspect of the breeding process is the identification of genetic features associated with plant response to pathogen exposure. Numerous clubroot resistance loci were described in Brassica crops (Landry et al, 1992;Figdore et al, 1993;Voorrips and Visser, 1993;Grandclément and Thomas, 1996;Voorrips et al, 1997;Matsumoto et al, 1998;Moriguchi et al, 1999;Suwabe et al, 2003Suwabe et al, , 2006Hirai et al, 2004;Laurens and Thomas, 2004;Piao et al, 2004;Rocherieux et al, 2004;Nomura et al, 2005;Saito et al, 2006;Sakamoto et al, 2008;Nagaoka et al, 2010;Kato et al, 2012;Chen et al, 2013;Chu et al, 2013Chu et al, , 2014Hatakeyama et al, 2013Hatakeyama et al, , 2017Pang et al, 2014Pang et al, , 2018Zhang et al, 2014;Fredua-Agyeman and Rahman, 2016;Lee et al, 2016;Huang et al, 2017;Yu et al, 2017;Dakouri et al, 2018;Hirani et al, 2018;Nguyen et al, 2018;Peng et al, 2018;Laila et al, 2019;Choi et al, 2020;Farid et al, 2020;Karim et al, 2020) and are reviewed in (Neik et al, 2017;…”

Section: Introductionmentioning

confidence: 99%

Local Duplication of TIR-NBS-LRR Gene Marks Clubroot Resistance in Brassica napus cv. Tosca

et al. 2021

View full text Add to dashboard Cite

Clubroot, caused by Plasmodiophora brassicae infection, is a disease of growing importance in cruciferous crops, including oilseed rape (Brassica napus). The affected plants exhibit prominent galling of the roots that impairs their capacity for water and nutrient uptake, which leads to growth retardation, wilting, premature ripening, or death. Due to the scarcity of effective means of protection against the pathogen, breeding of resistant varieties remains a crucial component of disease control measures. The key aspect of the breeding process is the identification of genetic factors associated with variable response to the pathogen exposure. Although numerous clubroot resistance loci have been described in Brassica crops, continuous updates on the sources of resistance are necessary. Many of the resistance genes are pathotype-specific, moreover, resistance breakdowns have been reported. In this study, we characterize the clubroot resistance locus in the winter oilseed rape cultivar “Tosca.” In a series of greenhouse experiments, we evaluate the disease severity of P. brassicae-challenged “Tosca”-derived population of doubled haploids, which we genotype with Brassica 60 K array and a selection of SSR/SCAR markers. We then construct a genetic map and narrow down the resistance locus to the 0.4 cM fragment on the A03 chromosome, corresponding to the region previously described as Crr3. Using Oxford Nanopore long-read genome resequencing and RNA-seq we review the composition of the locus and describe a duplication of TIR-NBS-LRR gene. Further, we explore the transcriptomic differences of the local genes between the clubroot resistant and susceptible, inoculated and control DH lines. We conclude that the duplicated TNL gene is a promising candidate for the resistance factor. This study provides valuable resources for clubroot resistance breeding programs and lays a foundation for further functional studies on clubroot resistance.

show abstract

Quantitative Trait Locus Mapping of Clubroot Resistance and Plasmodiophora brassicae Pathotype Banglim-Specific Marker Development in Brassica rapa

Cited by 10 publications

References 68 publications

MiR1885 Regulates Disease Tolerance Genes in Brassica rapa during Early Infection with Plasmodiophora brassicae

MiR1885 Regulates Disease Tolerance Genes in Brassica rapa during Early Infection with Plasmodiophora brassicae

Breeding for Disease Resistance in Brassica Vegetables Using DNA Marker Selection

Local Duplication of TIR-NBS-LRR Gene Marks Clubroot Resistance in Brassica napus cv. Tosca

Contact Info

Product

Resources

About