Quantitative Ultrastructural Analysis of the Neurofilament 200–Positive Axons in the Rat Dental Pulp

Paik, Sang Kyoo; Lee, Dae Seop; Kim, Jae Young; Bae, Jin Young; Cho, Yi Sul; Ahn, Dong Kuk; Yoshida, Atsushi; Bae, Yong Chul

doi:10.1016/j.joen.2010.05.005

Cited by 17 publications

(14 citation statements)

References 32 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Consequently, large CGRP+ neurons may have unmyelinated axons that branch extensively. This is supported by the observation that many TG neurons innervating the dental pulp are large, whereas most axons in the peripheral pulp are unmyelinated, with an extensive branching pattern (Sugimoto et al, ; Hildebrand et al, ; Paik et al, ).…”

Section: Discussionmentioning

confidence: 89%

“…Some myelinated fibers branch, lose their myelin, and thin out during their peripheral course to their terminal receptive fields (Nicol and Walmsley, ; Peng et al, ). Thus, about one‐half of the myelinated fibers in the peripheral sensory root of TG become unmyelinated within the initial part of the dental pulp (root pulp), and most become unmyelinated within the peripheral pulp that they innervate (Paik et al, ). For this reason, we analyzed the fiber types that express SP, CGRP, IB4, NF200, and Peripherin at the initial part of the peripheral sensory root of the TG, before the fibers have undergone these morphological changes.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Quantitative analysis of afferents expressing substance P, calcitonin gene‐related peptide, isolectin B4, neurofilament 200, and Peripherin in the sensory root of the rat trigeminal ganglion

Bae

Kim

Cho

et al. 2014

J of Comparative Neurology

Self Cite

View full text Add to dashboard Cite

Substance P (SP), calcitonin gene-related peptide (CGRP), and isolectin B4 (IB4) are widely used as markers for peripheral neurons with unmyelinated fibers, whereas neurofilament 200 (NF200), and Peripherin are used as markers for neurons with myelinated fibers, and with unmyelinated or small-caliber fibers, respectively. To study the selectivity of these markers for specific neuronal types, we analyzed their expression in neurons in the rat trigeminal ganglion by light- and electron-microscopic immunocytochemistry. Most SP-immunopositive (+), CGRP+, and IB4+ fibers were unmyelinated, but a small fraction (∼5%) were small myelinated fibers (<20 µm(2) in cross-sectional area, equivalent to <5 µm in diameter, Aδ fiber). Similarly, whereas the majority of NF200+ fibers were myelinated, a large fraction (23.9%) were unmyelinated, and whereas the majority of Peripherin+ fibers were unmyelinated and small myelinated, a significant fraction (15.5%) were large myelinated (>20 µm(2) in cross-sectional area, equivalent to >5 µm in diameter, Aβ fiber). Our findings confirm that SP, CGRP, and IB4 can be used as reliable markers for neurons with unmyelinated fibers, and question the suitability of NF200 as a marker for neurons with myelinated fibers, and of Peripherin as a marker for neurons with unmyelinated, or fine-caliber fibers.

show abstract

Section: Discussionmentioning

confidence: 89%

Section: Discussionmentioning

confidence: 99%

Quantitative analysis of afferents expressing substance P, calcitonin gene‐related peptide, isolectin B4, neurofilament 200, and Peripherin in the sensory root of the rat trigeminal ganglion

Bae

Kim

Cho

et al. 2014

J of Comparative Neurology

Self Cite

View full text Add to dashboard Cite

show abstract

“…Other findings suggest a thinning of fibers and progressive loss of the myelin sheath as axons course toward the tooth since the proportion of myelinated axons relative to unmyelinated axons is reduced in nerves closer to teeth when compared to more distant sites [30,31]. A progressive loss of myelin is also seen within the tooth since the proportion of unmyelinated axons is greater at more coronal locations than seen near the root apex in rat molars [32]. Additionally, the faster conduction velocities of action potentials recorded in extrapulpal segments when compared to intrapulpal locations is also suggestive of alterations in myelination status [33,34].…”

Section: Discussionmentioning

confidence: 99%

Unmyelinated nerve fibers in the human dental pulp express markers for myelinated fibers and show sodium channel accumulations

2012

View full text Add to dashboard Cite

Background The dental pulp is a common source of pain and is used to study peripheral inflammatory pain mechanisms. Results show most fibers are unmyelinated, yet recent findings in experimental animals suggest many pulpal afferents originate from fibers that are myelinated at more proximal locations. Here we use the human dental pulp and confocal microscopy to examine the staining relationships of neurofilament heavy (NFH), a protein commonly expressed in myelinated afferents, with other markers to test the possibility that unmyelinated pulpal afferents originate from myelinated axons. Other staining relationships studied included myelin basic protein (MBP), protein gene product (PGP) 9.5 to identify all nerve fibers, tyrosine hydroxylase (TH) to identify sympathetic fibers, contactin-associated protein (caspr) to identify nodal sites, S-100 to identify Schwann cells and sodium channels (NaChs). Results Results show NFH expression in most PGP9.5 fibers except those with TH and include the broad expression of NFH in axons lacking MBP. Fibers with NFH and MBP show NaCh clusters at nodal sites as expected, but surprisingly, NaCh accumulations are also seen in unmyelinated fibers with NFH, and in fibers with NFH that lack Schwann cell associations. Conclusions The expression of NFH in most axons suggests a myelinated origin for many pulpal afferents, while the presence of NaCh clusters in unmyelinated fibers suggests an inherent capacity for the unmyelinated segments of myelinated fibers to form NaCh accumulations. These findings have broad implications on the use of dental pulp to study pain mechanisms and suggest possible novel mechanisms responsible for NaCh cluster formation and neuronal excitability.

show abstract

“…The dental pulp is densely innervated by nociceptive afferents (10)(11)(12)(13) and is thus used frequently as a model system to study pain mechanisms. Glutamate signaling plays a crucial role in pain transduction (14,15).…”

mentioning

confidence: 99%

Vesicular Glutamate Transporters in Axons That Innervate the Human Dental Pulp

Paik

Kim

Choi

et al. 2012

Journal of Endodontics

Self Cite

View full text Add to dashboard Cite

Quantitative Ultrastructural Analysis of the Neurofilament 200–Positive Axons in the Rat Dental Pulp

Cited by 17 publications

References 32 publications

Quantitative analysis of afferents expressing substance P, calcitonin gene‐related peptide, isolectin B4, neurofilament 200, and Peripherin in the sensory root of the rat trigeminal ganglion

Quantitative analysis of afferents expressing substance P, calcitonin gene‐related peptide, isolectin B4, neurofilament 200, and Peripherin in the sensory root of the rat trigeminal ganglion

Unmyelinated nerve fibers in the human dental pulp express markers for myelinated fibers and show sodium channel accumulations

Vesicular Glutamate Transporters in Axons That Innervate the Human Dental Pulp

Contact Info

Product

Resources

About