Quantitative Untersuchungen an den Enzymen Lysozym und Phosphohexoseisomerase im Mischspeichel beim oralen Plattenepithelkarzinom

Meyer, P; Zechel, T.

doi:10.1007/s001060170058

Cited by 5 publications

(4 citation statements)

References 13 publications

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“…In der Literatur sind die Laktoferrin− und Lysozymkonzentration in bronchioalveolärer Lavage und im Sputum [7,24,30] beschrie− ben worden. Ein direkter Vergleich der Konzentrationen zwi− schen Geweben, bronchioalveoläre Lavage und Sputum ist je− doch nur bedingt sinnvoll, da die Proteinkonzentration im Spu− tum aus der Proteinabgabe der Speicheldrüsen und dem umlie− genden Gewebe resultiert.…”

Section: Diskussionunclassified

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Lysozym und Laktoferrin in Adenoiden, hyperplastischen und chronisch entzündeten Tonsillen - eine quantitative Analyse

Schwaab¹,

Euteneuer²,

Lautermann³

et al. 2005

Laryngo-Rhino-Otol

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Section: Diskussionunclassified

“…Lysozym wer− den Aufgaben wie antimikrobielle Abwehr, Bakteriolyse, Opso− nierung, Immunpotenzierung und Anti−Tumoraktivität zuge− schrieben [6]. Im Sputum gilt Lysozym als die Hauptkomponente der bakteriziden Wirkung [7].…”

unclassified

Lysozym und Laktoferrin in Adenoiden, hyperplastischen und chronisch entzündeten Tonsillen - eine quantitative Analyse

Schwaab¹,

Euteneuer²,

Lautermann³

et al. 2005

Laryngo-Rhino-Otol

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“…Low salivary amylase levels in preoperative patients with ruptured aortic aneurysm are associated with increased mortality. , Cigarette smokers have significantly reduced salivary amylase activity . Salivary amylase concentration is also measured to evaluate the damage to salivary glands during radiotherapy in the treatment of head and neck cancer. , In the mixed saliva of the oral squamous cell carcinoma patients, the lysozyme concentration was significantly increased ( p = 0.011) . Salivary lysozyme was also reported may associate with coronary artery disease (CAD). , …”

Section: Introductionmentioning

confidence: 99%

“…19,25 In the mixed saliva of the oral squamous cell carcinoma patients, the lysozyme concentration was significantly increased (p = 0.011). 26 Salivary lysozyme was also reported may associate with coronary artery disease (CAD). 27,28 In this work, salivary proteins with digestive functions (such as amylase, lipase, and lysozyme) were imprinted using a biocompatible polymer, poly(ethylene-co-vinyl alcohol).…”

Section: ' Introductionmentioning

confidence: 99%

Sensing of Digestive Proteins in Saliva with a Molecularly Imprinted Poly(ethylene-co-vinyl alcohol) Thin Film Coated Quartz Crystal Microbalance Sensor

Lee

Thomas

Tseng

et al. 2011

ACS Appl. Mater. Interfaces

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The quartz crystal microbalance (QCM) has a sensitivity comparable to that of the surface plasmon resonance (SPR) transducer. Molecularly imprinted polymers (MIPs) have a much lower cost than natural antibodies, they are easier to fabricate and more stable, and they exhibit satisfactory recognition ability when integrated onto sensing transducers. Hence, MIP-based QCM sensors have been used to recognize small molecules and, recently, microorganisms, but only a few have been adopted in protein sensing. In this work, a mixed salivary protein and poly(ethylene-co-vinyl alcohol), EVAL, solution is coated onto a QCM chip and a molecularly imprinted EVAL thin film formed by thermally induced phase separation (TIPS). The optimal ethylene mole ratios of the commercially available EVALs for the imprinting of amylase, lipase and lysozyme were found to be 32, 38, and 44 mol %, respectively. Finally, the salivary protein-imprinted EVAL-based QCM sensors were used to detect amylase, lipase and lysozyme in real samples (saliva) and their effectiveness was compared with that of a commercial ARCHITECT ci 8200 chemical analysis system. The limits of detection (LOD) for those salivary proteins were as low as ∼pM.

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Comparison of N-linked Glycoproteins in Human Whole Saliva, Parotid, Submandibular, and Sublingual Glandular Secretions Identified using Hydrazide Chemistry and Mass Spectrometry

et al. 2008

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Introduction Saliva is a body fluid that holds promise for use as a diagnostic fluid for detecting diseases. Salivary proteins are known to be heavily glycosylated and are known to play functional roles in the oral cavity. We identified N-linked glycoproteins in human whole saliva, as well as the N-glycoproteins in parotid, submandibular, and sublingual glandular fluids. Materials and Methods We employed hydrazide chemistry to affinity enrich for N-linked glycoproteins and glycopeptides. PNGase F releases the N-peptides/proteins from the agarose-hydrazide resin, and liquid chromatography–tandem mass spectrometry was used to identify the salivary N-glycoproteins. Results A total of 156 formerly N-glycosylated peptides representing 77 unique N-glycoproteins were identified in salivary fluids. The total number of N-glycoproteins identified in the individual fluids was: 62, 34, 44, and 53 in whole saliva, parotid fluid, submandibular fluid, and sublingual fluid, respectively. The majority of the N-glycoproteins were annotated as extracellular proteins (40%), and several of the N-glycoproteins were annotated as membrane proteins (14%). A number of glycoproteins were differentially found in submandibular and sublingual glandular secretions. Conclusions Mapping the N-glycoproteome of parotid, submandibular, and sublingual saliva is important for a thorough understanding of biological processes occurring in the oral cavity and to realize the role of saliva in the overall health of human individuals. Moreover, identifying glycoproteins in saliva may also be valuable for future disease biomarker studies.

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Quantitative Untersuchungen an den Enzymen Lysozym und Phosphohexoseisomerase im Mischspeichel beim oralen Plattenepithelkarzinom

Cited by 5 publications

References 13 publications

Lysozym und Laktoferrin in Adenoiden, hyperplastischen und chronisch entzündeten Tonsillen - eine quantitative Analyse

Lysozym und Laktoferrin in Adenoiden, hyperplastischen und chronisch entzündeten Tonsillen - eine quantitative Analyse

Sensing of Digestive Proteins in Saliva with a Molecularly Imprinted Poly(ethylene-co-vinyl alcohol) Thin Film Coated Quartz Crystal Microbalance Sensor

Comparison of N-linked Glycoproteins in Human Whole Saliva, Parotid, Submandibular, and Sublingual Glandular Secretions Identified using Hydrazide Chemistry and Mass Spectrometry

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