Quantitative variations of taxifolin and its glucoside in Pinus sylvestris needles consumed by Diprion pini larvae

Abstract

Summary — The relationships between quantitative variations of 2 flavanonols in Scots pine needles and Diprion pini larvae mortality were studied. Those 2 compounds were characterized as taxifolin (T) and its glucoside (TG) after hydrolysis and analysis by TLC, HPLC and spectrophotometry. Quantitative differences between 30 clones were more important for TG than for T, nevertheless clones which presented a content of taxifolin higher than 1.5 mg g -1 DW showed a T/TG ratio equal to or greater than 0.5 (fig 2). Quantitative changes were also observed throughout the year. The amount of taxifolin peaked in autumn as those of its glucoside decreased (fig 3). Darkness also induced a gradual increase of T but no significant effect on TG (fig 4). Storage of twigs during feeding tests and insect defoliation both induced a strong glucosilation of taxifolin in needles (table I). High rates of mortality of Diprion pini larvae were associated with the presence of T and TG both in needles and faeces ( (fig 3). De même, l'effet de l'obscurité sur les rameaux provoque une

Herbivores and variation in the composition of specific phenolics of boreal coniferous trees: a search for patterns

“…Chemical groups and PCs (in bold letters) are given at the bottom of the table (Auger et al 1994;Beninger and Abou-Zaid 1997) and deterrent properties against moose and hares feeding on willows and birch and voles feeding on pine (Bucyanayandi et al 1990;Laitinen et al 2004;Stolter et al 2005;Stolter 2008). Interestingly, P. sylvestris is a preferred food plant for mammalian herbivores, especially for moose, at least in comparison to other coniferous trees such as P. abies (e.g.…”

“…Cederlund et al 1980;Niemelä and Danell 1988;Faber 1998;Hörnberg 2001;Månsson et al 2007). The deterrent effects of flavonols might vary with dosage, composition or with synergetic effects between different substances (Larsson et al 1992;Auger et al 1994;Faccoli and Schlyter 2007;Witzell and Martin 2008). In contrast, the low acceptance of P. abies as food for mammalian herbivores might be due to other compounds than flavonoids.…”

“…Bryant et al 1983b;Auger et al 1994;Ikonen et al 2001;Berg 2003;Laitinen et al 2004;Stolter et al 2005;Faccoli and Schlyter 2007). Two main theories on the evolution of a variety of secondary plant metabolites exist.…”

Flavonoid Metabolites in the Hemolymph of European Pine Sawfly (Neodiprion sertifer) Larvae

“…These multiple roles make it difficult to interpret the precise function of flavonoids metabolites in sawfly larvae. Some flavonoids (e.g., taxifolin and its glycoside) have either negative or no effects on the performance of pine-feeding sawflies (Larsson et al, 1992;Auger et al, 1994). The high concentrations of flavonoid metabolites in the hemolymph of N. sertifer imply that these compounds may not be particularly harmful for the larvae.…”

Simultaneous identification of phenolic and flavonoid contents in bee pollen by HPLC-ESI-MS data

“…However, the aglycone could not be identified and it tentatively characterized as taxifolin diglycoside. This is a polyphenolic compound which commonly found in foods of plant origin and in pines of Pinus sylvestris [4] Compound 6 was characterized as flavonoid with monobenzoylated pyranose sugar due to the presence of molecular ion at m/z 582 (C 29 H 26 O 13 ) and fragment ions at m/z 462 (after loss of benzoyl group) ( Figure 7). This compound was identified as a new flavonoid based natural product and, to our knowledge, is here reported for the first time.…”