Quantitative Volumetric Perfusion Mapping of the Microvasculature Using Contrast Ultrasound

Feingold, Steven; Gessner, Ryan C.; Guracar, Ismayil M.; Dayton, Paul A.

doi:10.1097/rli.0b013e3181ef0a78

Cited by 90 publications

(85 citation statements)

References 18 publications

(23 reference statements)

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“…Subsequent studies have been performed in rat (13,31,41), rabbit (59), dog (63,64,68), swine (24,69), mouse (55,57), and isolated organs (54). Contrast ultrasound imaging of the kidney is not in widespread clinical use, although studies have demonstrated potential utility for evaluation of focal abnormalities (46) and in identifying rejection following renal transplant (14,27,28).…”

Section: Discussionmentioning

confidence: 99%

“…Much of the work in validating perfusion ultrasound imaging in the kidney has been performed using surgically (19,58,68) or pharmacologically (13,31,41,57) induced changes in renal blood flow but not in models of common clinically relevant diseases. To our knowledge, the data presented here are the first evidence that this technique can detect chronic kidney disease and its response to treatment.…”

Section: Discussionmentioning

confidence: 99%

“…Kidneys were evaluated in only one imaging plane, which may introduce variability due to undersampling (13). Volumetric imaging may therefore be required to achieve the greatest sensitivity, although at the expense of increased procedure time and complexity.…”

Section: Discussionmentioning

confidence: 99%

See 2 more Smart Citations

Effects of high-fat diet and losartan on renal cortical blood flow using contrast ultrasound imaging

Declèves

Rychak

Smith

et al. 2013

American Journal of Physiology-Renal Physiology

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Declèves AE, Rychak JJ, Smith DJ, Sharma K. Effects of high-fat diet and losartan on renal cortical blood flow using contrast ultrasound imaging. Am J Physiol Renal Physiol 305: F1343-F1351, 2013. First published September 18, 2013 doi:10.1152/ajprenal.00326.2013.-Obesity-related kidney disease occurs as a result of complex interactions between metabolic and hemodynamic effects. Changes in microvascular perfusion may play a major role in kidney disease; however, these changes are difficult to assess in vivo. Here, we used perfusion ultrasound imaging to evaluate cortical blood flow in a mouse model of high-fat diet-induced kidney disease. C57BL/6J mice were randomized to a standard diet (STD) or a high-fat diet (HFD) for 30 wk and then treated either with losartan or a placebo for an additional 6 wk. Noninvasive ultrasound perfusion imaging of the kidney was performed during infusion of a microbubble contrast agent. Blood flow within the microvasculature of the renal cortex and medulla was derived from imaging data. An increase in the time required to achieve full cortical perfusion was observed for HFD mice relative to STD. This was reversed following treatment with losartan. These data were concurrent with an increased glomerular filtration rate in HFD mice compared with STD-or HFD-losartan-treated mice. Losartan treatment also abrogated fibro-inflammatory disease, assessed by markers at the protein and messenger level. Finally, a reduction in capillary density was found in HFD mice, and this was reversed upon losartan treatment. This suggests that alterations in vascular density may be responsible for the elevated perfusion time observed by imaging. These data demonstrate that ultrasound contrast imaging is a robust and sensitive method for evaluating changes in renal microvascular perfusion and that cortical perfusion time may be a useful parameter for evaluating obesity-related renal disease. ultrasound contrast imaging; obesity-related kidney disease; renal perfusion THE GROWING EPIDEMIC OF OBESITY is a serious health and economic burden throughout the world. The consequence of obesity over time is characterized by insulin resistance, hyperglycemia, atherosclerosis, dyslipidemia, and hypertension. This cluster of risk factors referred to as metabolic syndrome occurs with 50% prevalence in patients 50-to 60-yr-old (4) and contributes to cardiovascular disease. Obesity is a major risk factor for diabetes and hypertension, which together account for ϳ70% of all cases of end-stage renal disease (6). However, there is growing evidence suggesting that obesity by itself may be an independent risk factor for the development of vascular dysfunction and renal disease (21,44,66). Obesity-related kidney disease occurs as a result of complex interactions between metabolic and hemodynamic factors and is characterized by changes of renal perfusion, vascular dysfunction, as well as albuminuria, glomerulosclerosis, and tubulointerstitial fibrosis (20,37,48,50). Alterations in blood flow within the cortex or the medulla ma...

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Effects of high-fat diet and losartan on renal cortical blood flow using contrast ultrasound imaging

Declèves

Rychak

Smith

et al. 2013

American Journal of Physiology-Renal Physiology

View full text Add to dashboard Cite

show abstract

“…Practitioners need to be competent in the intravenous administration of contrast agents, be familiar with contraindications and be able to manage any possible adverse effects within the medical and legal framework of their country [4,38]. We refer to the educational activities of the collaborating societies of this paper, the World Federation of Ultrasound in Medicine and Biology (WFUMB) [9,23,[93][94][95][96][97][98][99][100][101][102], the European Federation of Societies for Ultrasound in Medicine and Biology (EFSUMB) [1,3,4,11,37,38,49,50,74,79,[103][104][105][106][107][108][109][110][111][112][113][114][115][116][117][118][119] and the CEUS LI-RADS Working Group for Liver Imaging Reporting and Data System (LI-RADS®) [34][35][36][120]…”

Section: Education Qualificationmentioning

confidence: 99%

Untitled

2018

Ultrasound Int Open

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“…By using a linear motion the exact same 2D plane to accurately monitor longitudinal perfusion changes, which is not possible in clinical practice (32). They also highlight that 3D DCE US imaging techniques are needed to with an absolute variance of perfusion up to 22% in healthy rat kidneys (31). These results illustrate the dependence of 2D DCE US imaging on operator accuracy because the operator has to find Box-and-whisker plots show spatial heterogeneity of tumor perfusion for all nine human colon cancer xenografts made visible with DCE US imaging before (black box plots) and 24 hours after (red box plots) antiangiogenic therapy.…”

Section: Experimental Studiesmentioning

confidence: 99%

Three-dimensional Dynamic Contrast-enhanced US Imaging for Early Antiangiogenic Treatment Assessment in a Mouse Colon Cancer Model

Hristov¹,

Qin²,

Tian³

et al. 2015

Radiology

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q RSNA, 2015 Purpose:To evaluate feasibility and reproducibility of three-dimensional (3D) dynamic contrast material-enhanced (DCE) ultrasonographic (US) imaging by using a clinical matrix array transducer to assess early antiangiogenic treatment effects in human colon cancer xenografts in mice. Materials and Methods:Animal studies were approved by the Institutional Administrative Panel on Laboratory Animal Care at Stanford University. Three-dimensional DCE US imaging with two techniques (bolus and destruction-replenishment) was performed in human colon cancer xenografts (n = 38) by using a clinical US system and transducer. Twenty-one mice were imaged twice to assess reproducibility. Seventeen mice were scanned before and 24 hours after either antiangiogenic (n = 9) or saline-only (n = 8) treatment. Results:Reproducibility of both 3D DCE US imaging techniques was good to excellent (intraclass correlation coefficient, 0.73-0.86). PE, AUC, rBV, and rBF significantly decreased (P  .04) in antiangiogenic versus saline-treated tumors. rBV (r = 0.74; P = .06) and rBF (r = 0.85; P = .02) correlated with ex vivo percent area of blood vessels, although the statistical significance of rBV was not reached, likely because of small sample size. Overall, 2D DCE-US overestimated and underestimated treatment effects from up to 125-fold to170-fold compared with 3D DCE US imaging. If the central tumor plane was assessed, treatment response was underestimated up to threefold or overestimated up to 57-fold on 2D versus 3D DCE US images. Conclusion:Three-dimensional DCE US imaging with a clinical matrix array transducer is feasible and reproducible to assess tumor perfusion in human colon cancer xenografts in mice and allows for assessment of early treatment response after antiangiogenic therapy.q RSNA, 2015

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Quantitative Volumetric Perfusion Mapping of the Microvasculature Using Contrast Ultrasound

Cited by 90 publications

References 18 publications

Effects of high-fat diet and losartan on renal cortical blood flow using contrast ultrasound imaging

Effects of high-fat diet and losartan on renal cortical blood flow using contrast ultrasound imaging

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Three-dimensional Dynamic Contrast-enhanced US Imaging for Early Antiangiogenic Treatment Assessment in a Mouse Colon Cancer Model

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