Quantitatively linking collagen alteration and epithelial tumor progression by second harmonic generation microscopy

Zhuo, Shuangmu; Chen, Jianxin; Wu, Guizhu; Xie, Shuguang; Zheng, Liqin; Jiang, Xingshan; Zhu, Xiaodong

doi:10.1063/1.3441337

Cited by 81 publications

(86 citation statements)

References 13 publications

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“…In IBD, the metabolic pathway is still not completely understood due to differences in disease states (acute, chronic, active, quiescent) and the wide variation of both metabolic and structural factors involved, such as cytokines secretion, epithelia destruction and regeneration, inflammatory cells infiltrates, autophagy, etc [64,65]. It is therefore difficult to predict the changes in intracellular AF.…”

Section: Discussionmentioning

confidence: 99%

Fluorescence lifetime spectroscopy of tissue autofluorescence in normal and diseased colon measured ex vivo using a fiber-optic probe

Coda¹,

Thompson²,

Kennedy³

et al. 2014

Biomed. Opt. Express

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Abstract:We present an ex vivo study of temporally and spectrally resolved autofluorescence in a total of 47 endoscopic excision biopsy/resection specimens from colon, using pulsed excitation laser sources operating at wavelengths of 375 nm and 435 nm. A paired analysis of normal and neoplastic (adenomatous polyp) tissue specimens obtained from the same patient yielded a significant difference in the mean spectrally averaged autofluorescence lifetime −570 ± 740 ps (p = 0.021, n = 12). We also investigated the fluorescence signature of non-neoplastic polyps (n = 6) and inflammatory bowel disease (n = 4) compared to normal tissue in a small number of specimens. References and links1. R. Lambert, H. Saito, and Y. Saito, "High-resolution endoscopy and early gastrointestinal cancer...dawn in the East," Endoscopy 39(3), 232-237 (2007

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Section: Discussionmentioning

confidence: 99%

Fluorescence lifetime spectroscopy of tissue autofluorescence in normal and diseased colon measured ex vivo using a fiber-optic probe

Coda¹,

Thompson²,

Kennedy³

et al. 2014

Biomed. Opt. Express

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“…MPM was achieved using a nonlinear optical system which has been described previously (16). In brief, multiphoton images were acquired using a commercial laser scanning microscopic imaging system (Zeiss LSM 510 META; Carl Zeiss, Jena, Germany) coupled to a femtosecond Ti: sapphire laser (Mira 900-F, Coherent Inc., Santa Clara, CA, USA) operating at 800 nm.…”

Section: Methodsmentioning

confidence: 99%

Visualization of basement membranes in normal breast and breast cancer tissues using multiphoton microscopy

Chen

Qiu

et al. 2016

Oncology Letters

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Abstract.Since basement membranes represent a critical barrier during breast cancer progression, timely imaging of these signposts is essential for early diagnosis of breast cancer. A label-free method using multiphoton microscopy (MPM) based on two-photon excited fluorescence signals and second harmonic generation signals for analyzing the morphology of basement membrane in normal and cancerous breast tissues is likely to enable a better understanding of the pathophysiology of breast cancer and facilitate improved clinical management and treatment of this disease. The aim of this study was to determine whether MPM has the potential for label-free assessment of the morphology of basement membrane in normal and cancerous breast tissues. A total of 60 tissue section samples (comprising 30 fresh breast cancer specimens and 30 normal breast tissues) were first imaged (fresh, unfixed and unstained) with MPM and are then processed for routine hematoxylin and eosin (H&E) histopathology. Comparisons were made between MPM imaging and gold standard sections for each specimen stained with H&E. Simply by visualizing morphological features appearing on multiphoton images, cancerous lesions may be readily identified by the loss of basement membrane and tumor cells characterized by irregular size and shape, enlarged nuclei and increased nuclear-cytoplasmic ratio. These results suggest that MPM has potential as a label-free method of imaging the morphology of basement membranes and cell features to effectively distinguish between normal and cancerous breast tissues.

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“…It might be caused by the dehydration of tissue constituents and/ or the structural modification of collagen. 20 In conclusion, confocal micro-Raman spectroscopy provides a highly sensitive way to characterize DMSO diffusion coefficient in porcine skin. Our results confirm the following: (1) The diffusion coefficients of DMSO in porcine skin are varying at different depths of skin (different layer in porcine skin).…”

mentioning

confidence: 99%

Discrimination of dimethyl sulphoxide diffusion coefficient in the process of optical clearing by confocal micro-Raman spectroscopy

et al. 2013

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Quantitatively linking collagen alteration and epithelial tumor progression by second harmonic generation microscopy

Cited by 81 publications

References 13 publications

Fluorescence lifetime spectroscopy of tissue autofluorescence in normal and diseased colon measured ex vivo using a fiber-optic probe

Fluorescence lifetime spectroscopy of tissue autofluorescence in normal and diseased colon measured ex vivo using a fiber-optic probe

Visualization of basement membranes in normal breast and breast cancer tissues using multiphoton microscopy

Discrimination of dimethyl sulphoxide diffusion coefficient in the process of optical clearing by confocal micro-Raman spectroscopy

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