Quantitiative determination of glycosylated hemoglobin A1 by agar gel electrophoresis.

Ménard, Luc; Dempsey, Mary E.; Blankstein, Larry; Aleyassine, H.; Wacks, Morton E.; Soeldner, J S

doi:10.1093/clinchem/26.11.1598

Cited by 191 publications

(19 citation statements)

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“…The blood samples during the study of ambient blood-glucose concentration were collected onto boric acid-impregnated filter paper [23], and the spots were eluted into 2.5% trichloroacetic acid and estimated using a glucose oxidase method on a Cobas Bio Centrifugal Analyser (Roche Ltd., Welwyn Garden City, Hertfordshire, UK). In phase I of the study, glycated haemoglobin was measured by agar gel electrophoresis (Coming Medical Ltd, Halstead, Essex, UK) without prior removal of the labile Schiff base (total GHb-screening) [24]. In phase II, glycated haemoglobin was assayed by four methods -agar gel electrophoresis without removal of S chiff base (total GHb-recall) [24], agar gel electrophoresis with prior removal of Schiff base by incubation with dipotassium phthalate (stable GHb) [25], isolectric focusing (LKB, Bromma, Sweden) [26] and affinity chromatography on boronate gel (Glycogel B, Pierce and Warriner, Chester, Cheshire, UK) [27].…”

Section: Assay Methodsmentioning

confidence: 99%

Unexplained variability of glycated haemoglobin in non-diabetic subjects not related to glycaemia

Forrest²,

et al. 1990

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We have studied levels of glycated haemoglobin in a sample of 223 people aged over 40 years without known diabetes mellitus screened in a community study. Each had a glucose tolerance test and glycated haemoglobin measured by four methods - agar gel electrophoresis with and without removal of Schiff base, affinity chromatography and isoelectric focusing. The correlation coefficients between 2 h blood glucose and levels of glycated haemoglobin were between 0.43 and 0.64. This poor correlation was not explained on the basis of assay or biological variability of either 2 h blood glucose or glycated haemoglobin. Multiple regression analysis showed that other assays of glycated haemoglobin contributed to the variance of any single glycated haemoglobin value by 0.1%-52.9% (median 12.8%) compared to the variance of 18.6%-41.4% (median 30.8%) explained by 2 h blood glucose alone, suggesting that in a non-diabetic population, the degree of glucose intolerance may explain only one third of the variance of glycated haemoglobin levels, but other factors operate to produce consistent changes in levels of glycated haemoglobin. Investigation of 42 subjects with consistently high (20 subjects) or low (22 subjects) levels of glycated haemoglobin relative to their 2 h blood glucose level showed no difference in age, gender, body mass index, haemoglobin levels or smoking, although 50% of low glycators had impaired glucose tolerance. Neither ambient blood-glucose levels, as estimated on two five-point blood-glucose profiles, nor dietary intake of carbohydrate, starch, sugars, fibre or alcohol, explained the difference between high and low glycators. The determinants of the consistent interindividual differences in levels of glycated haemoglobin in non-diabetic subjects remain to be determined.

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Section: Assay Methodsmentioning

confidence: 99%

Unexplained variability of glycated haemoglobin in non-diabetic subjects not related to glycaemia

Forrest²,

et al. 1990

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“…The proportion of Asian, Mediterranean and other European, and Afrocaribbean subjects respectively was 1.8%, 10.0% and 9.6% in the practice population studied and 2.8%, 9.0% and 8.5% in the catchment population. A random sample of 1084 subjects older than 40 years of age was screened by a single capillary blood glucose level 2h after a 75 g glucose load and an estimation of glycohaemoglobin using an agar gel electrophoresis technique (Coming Medical Ltd., Halstead, Essex, UK) [15]. Subjects were ranked according to their levels of both glycohaemoglobin and 2 h blood glucose into separate strata.…”

Section: Methodsmentioning

confidence: 99%

The relationship of hospital admission and fatality from myocardial infarction to glycohaemoglobin levels

et al. 1988

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We have performed a study to assess the relative contributions of increased hospital admission rates with acute myocardial infarction and increased hospital case fatality to the excess mortality of subjects with elevated levels of glycohaemoglobin from myocardial infarction. Glycohaemoglobin levels were estimated by isoelectric focussing in 397 subjects without known diabetes mellitus admitted with myocardial infarction and compared with a control population reconstructed from a community sample of 1084 subjects without known diabetes mellitus screened in general practice. In the case-control comparison, glycohaemoglobin levels above the 90th centile were associated with relative risks of 3.1 (95% confidence interval 1.4-6.8) for admission with myocardial infarction and 5.3 (95% confidence interval 2.1-13.4) for death in hospital. Elevated glycohaemoglobin on admission was a predictor of both death and cardiac pump failure among those admitted with myocardial infarction, as was the presence of known diabetes. In those over 40 years of age, the top 1% of the glycohaemoglobin distribution contribute 4.3% of admissions and 9.6% of hospital deaths with myocardial infarction.

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“…The grafts were placed either under the left renal capsule (systemic drainage) or on to the hilar surface of the spleen (portal drainage) (Mandel, Hoffman and Carter, 1981). The effect of the grafts was followed by fortnightly measurement of body weight and RBG (glucose oxidase method, Autoanalyser 11), and GHb (Corning Glyctrac method (Menard et al, 1980)). Four months after grafting, when all animals had been euglycaemic for at least 2 months, an intraperitoneal arginine challenge was performed by giving 40 mg L-arginine in 0-2 ml of mouse tonicity phosphate-buffered saline intraperitoneally at time zero, following a 3 h fast (Fajans et at., 1967).…”

Section: Methodsmentioning

confidence: 99%

A Comparison of Portal Versus Systemic Venous Drainage in Murine Foetal Pancreatic Islet Transplantation

Mandel

1986

Aust J Exp Biol Med

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Summary.We have compared the efficiency of foetal islet graft growth and function in renal subcapsular (systemic drainage) and splenic (portal drainage) sites. Age-matched female BALB/c streptozotocin (STZ)-diabetic mice were grafted either under the left renal capsule or on to the hilar surface of the spleen with one half of a syngeneic 17-day gestation foetal -pancreas which had been in organ culture for 2 weeks. Gain in body weight, return to jiormoglycaemia and to normal glycosylated haemoglobin levels were not significantly different between the two groups. However, when an intraperitoneal arginine challenge was performed the portally grafted mice showed more normal responses than the systemically grafted mice. Although still not normal after the arginine challenge, mean blood glucose values were lower and serum insulin values higher in the spleen grafted animals than in the renal subcapsular graft group. The total amount of insulin was assayed by extraction of both the graft and the pancreas of each animal. The amount of insulin in the grafts from the spleen was not significantly different from those in the renal subcapsular site. We conclude that the efficiency of a graft draining into the portal circulation is greater than an equivalent sized graft draining systemically when a maxima! challenge is applied.

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Quantitiative determination of glycosylated hemoglobin A1 by agar gel electrophoresis.

Cited by 191 publications

References 0 publications

Unexplained variability of glycated haemoglobin in non-diabetic subjects not related to glycaemia

Unexplained variability of glycated haemoglobin in non-diabetic subjects not related to glycaemia

The relationship of hospital admission and fatality from myocardial infarction to glycohaemoglobin levels

A Comparison of Portal Versus Systemic Venous Drainage in Murine Foetal Pancreatic Islet Transplantation

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