Quantity Determination of Magnetic Particles Intravenously Administered to Mice Tissues Using Magnetization Measurements

Abstract

This paper presents a method that has been developed to determine the quantity of accumulated magnetic particles in mice tissues using magnetization measurements. Dispersions of platelet Fe 3 O 4 particles with sizes of 30-50nm and a saturation magnetization of approximately 80 Am 2 /kg were intravenously administered to mice. Primary tissues were dried to measure the magnetization. The amounts of Fe 3 O 4 particles accumulated in the tissues were obtained by dividing the magnetization of tissues by the magnetization of Fe 3 O 4 particles under a magnetic field of 39.8 kA/m. A remarkable accumulation of particles was observed in the liver and spleen, being supported by the observation of tissues using Prussian blue staining. Total Fe 3 O 4 particles accumulated in primary tissues was approximately 38-40 wt% and 40-44 wt% against the particles in administered dispersions with 3.0 wt% and 0.4 wt% contents,respectively. The method developed in the study is considered to be effective for verifying magnetic hyperthermia and thermoablation therapies, in which the quantity of accumulated particles directly reflects the heating power required for those therapies.

Effect of different molecular coatings on the heating properties of maghemite nanoparticles

“…34 In addition, the modification of the nanoparticles' surface is fundamental to ensure good colloidal stability and modulate the interparticle distance through the formation of the clusters. Therefore, several stabilizers have been proposed in the literature, among them: hydrophilic molecules bonded to the NPs' surface, such as dimercaptosuccinic acid (DMSA), [35][36][37][38][39] functionalized silanes, [40][41][42] carboxylic acids; 37,[43][44][45][46] polymers for NPs' encapsulations as dextran, [47][48][49] polyethylene glycol (PEG), [50][51][52] polyacrylic acid, [53][54][55][56] biopolymers, [57][58][59][60] , etc. (Table 1S).…”