Quantity of nuclear DNA in malignancies and benign lymphadenopathies associated with Epstein-Barr virus.

Lehtinen, Tuula; Lehtinen, Matti; Aine, Risto; Dammert, K; Kulomaa, P; Alavaikko, M; Leinikki, Pauli

doi:10.1136/jcp.42.7.699

Cited by 6 publications

(3 citation statements)

References 32 publications

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“…"~~'~~* Flow cytometric analysis has been used successfully to study the incidence of EBV receptors on B-lymphocytes in NPC29 and to assess the nuclear DNA content in a number of EBV-related tumors, including three tetraploid NPC. 30 We demonstrated that high-quality, reproducible flow cytometric analyses are readily possible from formalinfixed archival material of NPC specimens. To the best of our knowledge, our study is the first to systematically examine whether cellular DNA content in this disease has prognostic significance.…”

Section: Discussionmentioning

confidence: 99%

A clinical and flow cytometric analysis of patients with nasopharyngeal cancer

Costello

Mason

Collins

et al. 1990

Cancer

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Abnormal cellular DNA content, a hallmark of malignancy, is known to be an important prognostic factor in many human solid tumors; however, no data have been published on whether cellular DNA content carries prognostic significance for patients with nasopharyngeal cancer (NPC). Archival, formalin-fixed, paraffin-embedded pathology specimens representing pretreatment tissue biopsies from 55 patients (41 men and 14 women) with NPC were analyzed for cellular DNA content in a retrospective fashion from 1968 to 1988. Individual tumors were classified as either lymphoepithelioma, squamous cell, or anaplastic carcinoma, and were staged according to International Union Against Cancer (UICC) criteria. All patients were treated with curative intent using a 4 to 6 MeV linear accelerator to total doses ranging from 50 to 60 Gy in 4 to 6 weeks. The overall 5-year actuarial survival for all 55 patients was 44.4% (men, 41%; women, 52%). Survival by T stage was as follows: T1, 65%; T2, 51%; T3, 36%; and T4, 27%. Similarly, the 5-year survival rate declined as the bulk of nodal metastases increased: N0, 62%; N2, 50%; N3, 37%; and N1, 25%. Patients who had anaplastic carcinoma had a 5-year survival of 73%, those with lymphoepithelioma had a 60% survival, and those with squamous cell cancer (SCC) had a 30% survival. There was a statistically significant difference in 5-year survival between patients with SCC and those with nonkeratinizing histologies (P less than 0.05). In addition, there was a significant association between patients older than 40 years of age with SCC and patients younger than 40 years of age with nonkeratinizing malignancies (P less than 0.01). Of the 55 tumors successfully analyzed, 22 (40%) were diploid and 33 (60%) were aneuploid. The mean coefficient of variation (CV) of all 55 samples was 6.17%. There was no significant difference in 5-year survival between patients with diploid and those with aneuploid tumors (48% versus 42%). Furthermore, there was no statistically significant survival difference between aneuploid and diploid tumors within any one histologic subgroup. There was also no significant survival difference related to the DNA index. The results indicate that the extent of local tumor spread is still the most important prognostic factor for patients treated with radiotherapy for NPC. The data support the conclusion that patients with lymphoepithelioma and anaplastic carcinomas have a superior survival to patients with squamous cell carcinoma.(ABSTRACT TRUNCATED AT 400 WORDS)

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Section: Discussionmentioning

confidence: 99%

A clinical and flow cytometric analysis of patients with nasopharyngeal cancer

Costello

Mason

Collins

et al. 1990

Cancer

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“…a large number of retrospective studies have been reported. Although reports on the prognostic value of DNA aneuploidy are sometimes conflicting, for most of the solid tumors diploidy is associated with a better prognosis compared to aneuploid tumors (114,120,123). In non-Hodgkin's lymphoma, however, particularly cases with a higher frequency of DNA aneuploid cells have not shown this apparent relation between ploidy status and prognosis (124)(125)(126)(127).…”

Section: 23mentioning

confidence: 99%

“…Although several chromosomes or breakpoints were overrepresented no special patterns could be recognized. Studies of DNA content in Hodgkin's disease as detected by DNA flow cytometry have generally yielded negative or disappointing results (110)(111)(112)(113)(114). In one study, an anti-nucleolar polyclonal antibody was applied to select for nuclei, that presumably are of the RS/H type (115); this study contained only 15 patients who all showed DNA aneuploidy.…”

Section: Introductionmentioning

confidence: 99%

Hodgkin's disease : clinical and biological determinants of prognosis

Erdkamp¹

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Accumulation of p53 protein correlates with tumour proliferative activity in EBV positive Burkitt's lymphoma

Lehtinen

Isola²,

Aine

et al. 1992

Hematological Oncology

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p53 tumour suppressor gene is often found mutated in Burkitt's lymphoma (BL) cell lines and tumours. We analysed 35 BL tumours for the accumulation of p53 protein, and correlated the results with DNA flow cytometric data on the proliferative activity (SPF), and data on the presence or absence of Epstein-Barr virus (EBV) DNA. More than one-third (37 per cent) of the tumours showed accumulation of p53, which was considered to be consistent with mutation of the p53 gene. Tumours that were positive both for EBV DNA and p53 had significantly higher mean SPF than corresponding EBV DNA negative and/or p53 negative tumours. The proportions of tumour cells with accumulation of p53 appeared to correlate with tumour SPF only in EBV DNA positive BLs. However, there was no apparent association between accumulation of p53 and the presence or absence of EBV DNA. These findings are suggestive of multiple pathways in BL tumour progression.

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Quantity of nuclear DNA in malignancies and benign lymphadenopathies associated with Epstein-Barr virus.

Cited by 6 publications

References 32 publications

A clinical and flow cytometric analysis of patients with nasopharyngeal cancer

A clinical and flow cytometric analysis of patients with nasopharyngeal cancer

Hodgkin's disease : clinical and biological determinants of prognosis

Accumulation of p53 protein correlates with tumour proliferative activity in EBV positive Burkitt's lymphoma

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