Quantum dot amplified impedimetric aptasensor for interferon-gamma

Januarie, Kaylin Cleo; Oranzie, Marlon; Feleni, Usisipho; Iwuoha, Emmanuel I.

doi:10.1016/j.electacta.2023.142825

Cited by 5 publications

(4 citation statements)

References 67 publications

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“…This section describes nanomaterials that are somewhat less common but are also successfully used to create impedimetric biosensors. Such materials include colloidal quantum dots [152,153], which during the synthesis process are formed with a shell of ligand molecules, two-dimensional nanomaterials from other semiconductor compounds [154][155][156], and, finally, magnetic nanoparticles [157,158].…”

Section: Other Nanomaterialsmentioning

confidence: 99%

“…Chemical modifications can be used to improve the sensitivity of aptasensors and improve the immobilization of ligands on the electrode surface. Januarie et al [152] proposed an aptasensor for the detection of interferon-gamma, a biomarker of tuberculosis, based on SnTeSe metal dichalcogenide quantum dots (Figure 19). The surface of the quantum dots is modified with short chain L-cysteine peptides to improve stability and solubility.…”

Section: Aptamer-based Impedimetric Biosensorsmentioning

confidence: 99%

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Hybrid Impedimetric Biosensors for Express Protein Markers Detection

Sitkov,

Ryabko,

Moshnikov

et al. 2024

Micromachines

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Impedimetric biosensors represent a powerful and promising tool for studying and monitoring biological processes associated with proteins and can contribute to the development of new approaches in the diagnosis and treatment of diseases. The basic principles, analytical methods, and applications of hybrid impedimetric biosensors for express protein detection in biological fluids are described. The advantages of this type of biosensors, such as simplicity and speed of operation, sensitivity and selectivity of analysis, cost-effectiveness, and an ability to be integrated into hybrid microfluidic systems, are demonstrated. Current challenges and development prospects in this area are analyzed. They include (a) the selection of materials for electrodes and formation of nanostructures on their surface; (b) the development of efficient methods for biorecognition elements’ deposition on the electrodes’ surface, providing the specificity and sensitivity of biosensing; (c) the reducing of nonspecific binding and interference, which could affect specificity; (d) adapting biosensors to real samples and conditions of operation; (e) expanding the range of detected proteins; and, finally, (f) the development of biosensor integration into large microanalytical system technologies. This review could be useful for researchers working in the field of impedimetric biosensors for protein detection, as well as for those interested in the application of this type of biosensor in biomedical diagnostics.

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Section: Other Nanomaterialsmentioning

confidence: 99%

Section: Aptamer-based Impedimetric Biosensorsmentioning

confidence: 99%

Hybrid Impedimetric Biosensors for Express Protein Markers Detection

Sitkov,

Ryabko,

Moshnikov

et al. 2024

Micromachines

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“…The practical application of this aptasensor was realized in spiked pleural fluid samples, and a relatively low detection limit of 0.09 pg/mL and a sensitivity of 14.79 ± 0.396°•(pg/mL) were achieved. The aptasensor was constructed with a specific and highly selective thiolated aptamer which was shown in a previous work (Januarie et al, 2023) to not exhibit signals in the presence of other proteins (i.e., interfering species), such as Scheme 1. Illustration of the aptasensor fabrication strategy based on the gold-thiol interactions between the PProDOT, AuNPs, and the thiol-modified anti-IFN-γ aptamer.…”

Section: Conclusion and Future Recommendationsmentioning

confidence: 99%

“…The practical application of this aptasensor was realized in spiked pleural fluid samples, and a relatively low detection limit of 0.09 pg/mL and a sensitivity of 14.79 ± 0.396 • •(pg/mL) were achieved. The aptasensor was constructed with a specific and highly selective thiolated aptamer which was shown in a previous work (Januarie et al, 2023) to not exhibit signals in the presence of other proteins (i.e., interfering species), such as interleukin-2 (IL-2, 120 pg/mL), immunoglobulin G (IgG, 120 pg/mL), human serum albumin (HSA, 120 pg/mL), bovine serum albumin (BSA, 120 pg/mL), and interferon gamma (10 pg/mL) [35]. Due to the association of IFN-γ levels with diverse diseases and their progression stages, this aptasensor could be used for the early detection of diseases linked to IFN-γ release.…”

Section: Conclusion and Future Recommendationsmentioning

confidence: 99%

Bode Phase Angle Signaling of a TB Disease Biomarker

Sidwaba,

Januarie,

Mini

et al. 2023

Molecules

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Tuberculosis (TB) is a worldwide burden whose total control and eradication remains a challenge due to factors including false positive/negative diagnoses associated with the poor sensitivity of the current diagnostics in immune-compromised and post-vaccinated individuals. As these factors complicate both diagnosis and treatment, the early diagnosis of TB is of pivotal importance towards reaching the universal vision of a TB-free world. Here, an aptasensor for signaling an interferon gamma (IFN-γ) TB biomarker at low levels is reported. The aptasensor was assembled through gold–thiol interactions between poly(3,4-propylenedioxythiophene), gold nanoparticles, and a thiol-modified DNA aptamer specific to IFN-γ. The aptasensor sensitively detected IFN-γ in spiked pleural fluid samples with a detection limit of 0.09 pg/mL within a linear range from 0.2 pg/mL to 1.2 pg/mL. The good performance of the reported aptasensor indicates that it holds the potential for application in the early diagnosis of, in addition to TB, various diseases associated with IFN-γ release in clinical samples.

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IFN‐γ and IL‐10 Immunosensor with Vertically Aligned Carbon Nanotube Interdigitated Electrodes toward Pen‐Side Cattle Paratuberculosis Monitoring

Ding,

Brownlee,

Parate

et al. 2024

Global Challenges

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Highly sensitive vertically aligned carbon nanotube arrays (VANTAs) interdigitated electrode (IDE) arrays are developed for electrochemical biosensing of two cytokines (i.e., interleukin‐10 (IL‐10) and interferon‐gamma (IFN‐γ)) that are useful for early detection Johne's disease (Bovine Paratuberculosis) in cattle. The high aspect ratio VANTA‐IDEs (50–60 µm in height) are grown through a chemical vapor deposition process from an iron (Fe) catalyst that is lithographically patterned on a silicon wafer with equal finger width and inter‐finger spacing of 25 µm. After functionalization with distinct antibodies the VANTA‐IDEs are capable of selective detection of both IL‐10 and IFN‐γ within an actual biological matrix (i.e., diluted bovine implant supernatant) over concentration ranges of 0.1 to 30 pg mL−1 (limit of detection – LOD: 0.0911 pg mL−1) and 50–500 pg mL−1 (LOD: 24.17 pg mL−1), respectively with a response time of <35 min. Results demonstrate important initial steps for rapid, pen‐side identification of cattle with stage‐I Mycobacterium avium subspecies paratuberculosis infection before physical symptoms of Johne's disease are present. Such a rapid pen‐side diagnostic test can be used on cattle at an auction or before they are introduced to a herd to ensure the larger population does not become infected with Johne's disease.

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Quantum dot amplified impedimetric aptasensor for interferon-gamma

Cited by 5 publications

References 67 publications

Hybrid Impedimetric Biosensors for Express Protein Markers Detection

Hybrid Impedimetric Biosensors for Express Protein Markers Detection

Bode Phase Angle Signaling of a TB Disease Biomarker

IFN‐γ and IL‐10 Immunosensor with Vertically Aligned Carbon Nanotube Interdigitated Electrodes toward Pen‐Side Cattle Paratuberculosis Monitoring

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