Quantum dot encapsulation in virus-like particles with tuneable structural properties and low toxicity

Tagit, Oya; Ruiter, Mark V. de; Brasch, Melanie; Ma, Yujie; Cornelissen, Jeroen J. L. M.

doi:10.1039/c7ra06684h

Cited by 26 publications

(14 citation statements)

References 53 publications

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“…Encapsulation and functionalizing cubic iron oxide NPs in BMV (Huang et al, ) and fluorescent polyanionic semiconducting polymer in CCMV (Ng, Chan, Lin, & Tolbert, ) generated active PVNPs for imaging. Chemical and genetic manipulation with nanoassembly of CCMV capsids can be adapted to encapsulation of inorganic nanoparticles such as quantum dots (Tagit et al, ), or Prussian blue nanoparticles (Wu, Yang, & Shin, ), to obtain functional nanohybrid materials with tunable sizes and different functions. CCMV carrying gold nanoparticles have been used to reduce nitrogen (the reduction sequence is nitrogen → hydroxylamine → amine) (A. Liu, Traulsen, & Cornelissen, ).…”

Section: Cargos For Loading In Pvnps and Applicationsmentioning

confidence: 99%

Plant viral nanoparticles for packaging and in vivo delivery of bioactive cargos

Shahgolzari

Pazhouhandeh

Milani

et al. 2020

WIREs Nanomed Nanobiotechnol

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Nanoparticles have unique capabilities and considerable promise for many different biological uses. One capability is delivering bioactive cargos to specific cells, tissues, or organisms. Depending on the task, there are multiple variables to consider including nanoparticle selection, targeting strategies, and incorporating cargo so it can be delivered in a biologically active form. One nanoparticle option, genetically controlled plant viral nanoparticles (PVNPs), is highly uniform within a given virus but quite variable between viruses with a broad range of useful properties. PVNPs are flexible and versatile tools for incorporating and delivering a wide range of small or large molecule cargos. Furthermore, PVNPs can be modified to create nanostructures that can solve problems in medical, environmental, and basic research. This review discusses the currently available techniques for delivering bioactive cargos with PVNPs and potential cargos that can be delivered with these strategies. This article is categorized under: Implantable Materials and Surgical Technologies > Nanomaterials and Implants Biology‐Inspired Nanomaterials > Protein and Virus‐Based Structures

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Section: Cargos For Loading In Pvnps and Applicationsmentioning

confidence: 99%

Plant viral nanoparticles for packaging and in vivo delivery of bioactive cargos

Shahgolzari

Pazhouhandeh

Milani

et al. 2020

WIREs Nanomed Nanobiotechnol

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“…Thereby, these VNPs are noninfectious and act only as nanocarriers as they are replication-incompetent . VNPs have been studied previously to encapsulate inorganic nanomaterials like quantum dots (QDs), gold, and magnetic NPs for bioimaging. , In addition, VNPs with fusogenic envelope protein are of great interest as they enter target cells by receptor-mediated endocytosis and fuse with the endosomal membrane by low-pH-induced conformation change to release cargo within their cytoplasm. , In this context, the fusogenic envelope protein-based VNPs have been reported earlier to deliver iron oxide NPs and radionuclides for in vivo high-resolution positron emission tomography (PET) and magnetic resonance imaging (MRI). − …”

Section: Introductionmentioning

confidence: 99%

Fusogenic Viral Protein-Based Near-Infrared Active Nanocarriers for Biomedical Imaging

Bishnoi

Kumari

Rehman

et al. 2021

ACS Biomater. Sci. Eng.

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An effective drug delivery system (DDS) relies on an efficient cellular uptake and faster intracellular delivery of theranostic agents, bypassing the endosomal mediated degradation of the payload. The use of viral nanoparticles (VNPs) permits such advancement, as the viruses are naturally evolved to infiltrate the host cells to deliver their genetic material. As a proof of concept, we bioengineered the vesicular stomatitis virus glycoprotein (VSV-G)-based near-infrared (NIR) active viral nanoconstructs (NAVNs) encapsulating indocyanine green dye (ICG) for NIR bioimaging. NAVNs are spherical in size and have the intrinsic cellular-fusogenic properties of VSV-G. Further, the NIR imaging displaying higher fluorescence intensity in NAVNs treated cells suggests enhanced cellular uptake and delivery of ICG by NAVNs compared to the free form of ICG. The overall study highlights the effectiveness of VSV-G-based VNPs as an efficient delivery system for NIR fluorescence imaging.

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“…Self-assembled viral protein cages have attracted great interest because of their controlled assembly and disassembly properties as well as their potential as biomolecular scaffolds with a highly organized structure . They are widely used as drug carriers and nanoreactors. − CCMV is a member of the Bromoviridae family, a plant virus with an icosahedral shell of 28 nm outer diameter and 18 nm inner diameter that surrounds a central core of single-stranded viral RNA .…”

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confidence: 99%

Self-Assembly of Viral Capsid Proteins Driven by Compressible Nanobubbles

Zhang

Cao

Liu

et al. 2020

J. Phys. Chem. Lett.

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Colloidal nanobubbles occur in gas-saturated aqueous solutions following high power water electrolysis. Here the influence of nanobubble solutions on the self-assembly properties of viral capsid proteins (CP) was investigated. Interestingly, we found that gas solutions were able to trigger the self-assembly of CP of cowpea chlorotic mottle virus (CCMV) in the absence of the viral genome, most likely by acting as a negatively charged template. The process was demonstrated by three distinct techniques, namely, dynamic light scattering (DLS), atomic force microscopy (AFM), and transmission electron microscopy (TEM). Furthermore, nanobubble-induced self-assembly of viral CP was found to depend on protein concentration. Low CP concentrations led to assembly of 18 nm virus-like particles (VLPs), comparable to T = 1 (Casper and Klug triangulation number) virus capsids, whereas high CP concentrations led to 28 nm VLPs (similar to T = 3 capsids). This paves a new route for self-assembly of VLPs.

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Quantum dot encapsulation in virus-like particles with tuneable structural properties and low toxicity

Abstract: Quantum dot encapsulation within cowpea chlorotic mottle virus-based capsid proteins to obtain size-tuneable, non-toxic, luminescent imaging probes is presented.

Cited by 26 publications

References 53 publications

Plant viral nanoparticles for packaging and in vivo delivery of bioactive cargos

Plant viral nanoparticles for packaging and in vivo delivery of bioactive cargos

Fusogenic Viral Protein-Based Near-Infrared Active Nanocarriers for Biomedical Imaging

Self-Assembly of Viral Capsid Proteins Driven by Compressible Nanobubbles

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