Quantum dots based in-vitro co-culture cancer model for identification of rare cancer cell heterogeneity

Abstract

Cancer cell heterogeneity (CCH) is crucial in understanding cancer progression and metastasis. The CCH is one of the stumbling blocks in modern medicine's therapeutics and diagnostics . An in-vitro model of co-culture systems of MCF-7, HeLa, HEK-293, with THP-1 cells showed the occurrence of EpCAM positive (EpCAM+) and EpCAM negative (EpCAM−) heterogenetic cancer cell types labeled with the Quantum Dot antibody conjugates (QDAb). This in-vitro model study could provide insights into the role of rare cancer cells manifestation and their heterogeneity in metastatic progression and risk for severe infections in these patients. We successfully report the presence of CCH based on the fluorescence ratios of the co-cultured cancer cells when treated with the QDAb. These short-term mimic co-cultures give a compelling and quite associated model for assessing early treatment responses in various cancers.

Deciphering breast cancer cell heterogeneity: a quantum dot-conjugate approach employing MCF-7 and THP-1 co-cultures

“…1b and c, and these results are consistent with earlier reports. 27,31,32 The results suggest that the core-shell QD l that were synthesized are highly crystalline and exhibit intense emission intensities (refer to ESI, † Table S1).…”

“…The detailed preparation of the quantum dot antibody conjugates (QD l/Ab ) is explained in the ESI † (Section S.1). All of the QD l/Ab are prepared in the sequential process, including the synthesis of quantum dots (QD l ) with fluorescence emissions at 450 nm (blue-QD 450 ), 525 nm (green-QD 525 ), and 615 nm (red-QD 615 ), as shown in the earlier research of Vyshnava et al 27,30,31,67 Later, the surfaces of the QD l were modified with MPA using the biphasic ligand exchange to form active -COO À (QD l/MPA ). The EDC/NHS chemical coupling chemical reaction was acquired for (PEG) on the surface of the QD l/MPA (QD l/MPA/PEG ) to form stable and low cytotoxicity.…”

“…The culture medium was replenished, and the prepared QD l/Ab was incubated in the wells according to the optimized protocol. 27,31 The cells were washed with 1X PBS and allowed to settle for 5 min before being treated with EDTA solution, which is less harmful to cells than trypsin, and then suspended in 1X PBS at 4 1C. Using a multilaser scan flow cytometry and sorting experiment, the fluorescence intensity of each QD l/Ab binding was then assessed with a flow cytometer (FACS Jazz, Becton Dickinson Co.).…”

Anti-cancer activity of crude Slrp protein conjugated mesoporous silica nanoparticles in HeLa Cell Lines: An in vitro approach

“…Further the MSN MPA were allowed to conjugate with the crude SlrP protein using EDC/NHS coupling chemistry MES buffer based on earlier established protocols (Vyshnava et al, 2022b;Lin et al, 2019). Initially, 10 mg of the MSN MPA was evenly distributed in 5 mL of MES buffer (pH 6.0) to create a uniform mixture.…”

Applications of Quantum Dots in Preventive Oncology

“…Quantum dots are also useful tools for developing and testing pharmaceuticals. They can be used to study drug behavior, efficacy, and safety in in vitro cell culture and in vivo animal models [ 97 , 98 ]. Despite the fact that targeted and controlled release are just two benefits that QDs have to offer in terms of drug delivery, toxicity, and biodegradability, body clearance issues still need to be addressed [ 99 , 100 ].…”