Quantum dots based molecular beacons for in vitro and in vivo detection of MMP-2 on tumor

Li, Xin; Deng, Dawei; Xue, Jianpeng; Qu, Lingzhi; Achilefu, Samuel; Gu, Yueqing

doi:10.1016/j.bios.2014.05.035

Cited by 78 publications

(51 citation statements)

References 21 publications

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“…They developed a 535 nm emitting QD donor-based FRET probes (using Rhodamine B as acceptor) for in vitro and a 720 nm emitting QD donor-based FRET sensor (using an NIR emitting dye as acceptor) for in vivo use. 298 Hu et al developed a mercury biosensor based on FRET from N-acetyl-L-cysteine stabilized QDs to a Rhodamine 6G derivative-mercury conjugate. They could show intracellular imaging of mercury.…”

Section: Cell Environmentmentioning

confidence: 99%

Quantum dots: bright and versatile in vitro and in vivo fluorescence imaging biosensors

2015

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Semiconductor quantum dots (QDs) have become important fluorescent probes for in vitro and in vivo bioimaging research. Their nanoparticle surfaces for versatile bioconjugation, their adaptable photophysical properties for multiplexed detection, and their superior stability for longer investigation times are the main advantages of QDs compared to other fluorescence imaging agents. Here, we review the recent literature dealing with the design and application of QD-bioconjugates for advanced in vitro and in vivo imaging. After a short summary of QD preparation and their most important properties, different QD-based imaging applications will be discussed from the technological and the biological point of view, ranging from super-resolution microscopy and single-particle tracking over in vitro cell and tissue imaging to in vivo investigations. A substantial part of the review will focus on multifunctional applications, in which the QD fluorescence is combined with drug or gene delivery towards theranostic approaches or with complementary technologies for multimodal imaging. We also briefly discuss QD toxicity issues and give a short outlook on future directions of QD-based bioimaging.

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Section: Cell Environmentmentioning

confidence: 99%

Quantum dots: bright and versatile in vitro and in vivo fluorescence imaging biosensors

2015

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“…Compared to other reported methods for detection of protein‐based biomarkers by utilizing proteolytic activity, the platform proposed here offers a faster response of a lower LOD. [ 31,47 ] Chen et al [ 31 ] proposed a localized surface plasmon resonance (LSPR) based sensor using peptide‐functionalized AuNPs with a LOD similar to this work. However, the detection time of our sensing platform was <1 min as compared to 4 min in the LSPR‐based sensors.…”

Section: Protein Sensing Characterization Of Nanoprobesmentioning

confidence: 90%

“…However, the detection time of our sensing platform was <1 min as compared to 4 min in the LSPR‐based sensors. [ 31 ] In another study, Li et al [ 47 ] used cadmium–tellurium–sulfur quantum dot molecular beacons based on fluorescence resonance energy transfer for in vitro and in vivo studies where the reported detection time was in the order of 1 h.…”

Section: Protein Sensing Characterization Of Nanoprobesmentioning

confidence: 99%

Nanoassembled Peptide Biosensors for Rapid Detection of Matrilysin Cancer Biomarker

Behi

Naficy

Chandrawati

et al. 2020

Small

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high translational values. [7][8][9] Commonly, organic fluorophore dyes and quantum dots (QDs) have been employed in order to modify peptides such as Escherichia coli for fluorescent imaging and biosensing. [10,11] Still, fluorescent dyes have several issues such as susceptibility to photobleaching and slender specificity for bioconjugation to particular reactive sites. [12,13] Most QDs, on the other hand, suffer from cytotoxicity for biomedical applications. [14][15][16] Carbon quantum dots (CDs), [17] a class of 0D carbon nanomaterials (1-10 nm) with unique optical properties, high photo stability, excellent biocompatibility, and facile preparation are promising substitutes to fluorescent dyes and QDs. [18,19] In addition, gold nanoparticles (AuNPs) have shown remarkable physical and optical properties in nanomedicine applications, specifically in the field of nanobiosensors. [20][21][22] The chemical and physical interactions between AuNPs and proteins or peptides have already been characterized with biophysical, [23] biochemical, [24] and computational methods, [25] which indicate the diversity of AuNPs as an ideal model material for developing new peptide/protein optical biosensors. So far, several studies have been conducted aiming at manipulating peptide self-nanoassembly into desired nanoarchitectures for sensing applications. [26][27][28][29][30][31] For instance, peptide-functionalized AuNPs with tunable, reversible, [32] and irreversible [33,34] aggregations were synthesized as nanobiosensors to detect phospholipases and proteolytic activities, respectively. Yet, it remains a challenge to fully engineer the nanoarchitecture of peptide and hybrid nanoparticles to respond to minuscule concentrations of target analytes (e.g., cancer biomarker) in a short amount of time.In the light of the necessity for rapid detection of cancerrelated protein biomarkers, we have engineered a sensitive and selective fluorescent, label-free protein detection platform hereafter referred to as the PACD (peptide-Au-carbon dots) nanoprobe (Sections S1-S7, Supporting Information). We based this class of hybrid nanobiosensing platform on a synthetic polypeptide aptamer sensitive to the target biomarker which is anchored to AuNPs from one end and semiconducting zero dimensional CDs from the other end with niche electro-optical properties (Figure 1a). The polypeptide, referred to as the bioreceptor interchangeably, belongs to a family of helix-loophelix polypeptides de novo (JR2EC). [35] The bioreceptors and the surface-functionalized CDs were assembled step-wise on the surface of AuNPs, forming a stable nanoprobe (Figure 1b). As the active component of the nanobiosensors, JR2ECEarly detection of cancer is likely to be one of the most effective means of reducing the cancer mortality rate. Hence, simple and ultra-quick methods for noninvasive detection of early-stage tumors are highly sought-after. In this study, a nanobiosensing platform with a rapid response time of nearly 30 s is introduced for the detection of matrilysin-the sal...

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“…Fluorogenic substrates have been used to determine the activity of coronavirus proteases and screen inhibitors (Kuo et al, 2004;Lee et al, 2014;Park et al, 2017;Song et al, 2014;Tomar et al, 2015;Wang et al, 2016;Yang et al, 2005;Zhao et al, 2008). Some recently described arrangements employ nanoparticles (Feltrup and Singh, 2012;Khalilzadeh et al, 2016;Udukala et al, 2016;Wang et al, 2014;Zeng et al, 2015) or quantum dot bioconjugates (Lee and Kim, 2015;Li et al, 2014;Medintz et al, 2006) with immobilized fluorescently or luminescently labeled peptide substrates. Alternatively, cleavage products may be monitored by analysis of proteolytic products by mass spectrometric methods (Hu et al, 2015;Joshi et al, 2017;Lathia et al, 2011;Rumlová et al, 2003), analytical HPLC (Teruya et al, 2016), or electrochemical methods based on the difference in penetration of substrate and cleavage products through the membrane of a polyionselective sensor (Gemene and Meyerhoff, 2011;Han et al, 1996).…”

Section: Assay and Methods For Screening And Evaluating Viral Maturatmentioning

confidence: 99%

In vitro methods for testing antiviral drugs

Rumlová

Ruml

2018

Biotechnology Advances

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Despite successful vaccination programs and effective treatments for some viral infections, humans are still losing the battle with viruses. Persisting human pandemics, emerging and re-emerging viruses, and evolution of drug-resistant strains impose continuous search for new antiviral drugs. A combination of detailed information about the molecular organization of viruses and progress in molecular biology and computer technologies has enabled rational antivirals design. Initial step in establishing efficacy of new antivirals is based on simple methods assessing inhibition of the intended target. We provide here an overview of biochemical and cell-based assays evaluating the activity of inhibitors of clinically important viruses.

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Quantum dots based molecular beacons for in vitro and in vivo detection of MMP-2 on tumor

Cited by 78 publications

References 21 publications

Quantum dots: bright and versatile in vitro and in vivo fluorescence imaging biosensors

Quantum dots: bright and versatile in vitro and in vivo fluorescence imaging biosensors

Nanoassembled Peptide Biosensors for Rapid Detection of Matrilysin Cancer Biomarker

In vitro methods for testing antiviral drugs

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