Quantum Dots in Flow Cytometry

Abrams, Barnaby; Dubrovsky, Tim

doi:10.1385/1-59745-369-2:185

Cited by 6 publications

(5 citation statements)

References 20 publications

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“…However, photostability, cytotoxicity and membrane permeability are limiting fluorophore-specific attributes and thus have to be considered carefully. In the last years, several studies focussed on quantum dot (QD) usage for FACS analysis instead of traditional fluorophores due to their narrower emission peaks (Abrams and Dubrovsky 2007) but their application is also limited in cause of often enhanced cytotoxicity (Derfus et al 2004). In this context, nanoparticles recently got in the focus of interest as revolutionary and alternative marker compared to conventional fluorophore dyes.…”

Section: Nano-revolution Of Genetics and Reproductive Biologymentioning

confidence: 99%

Golden Perspective: Application of Laser‐Generated Gold Nanoparticle Conjugates in Reproductive Biology

Barchanski

Taylor

Klein

et al. 2011

Reprod Domestic Animals

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Contents The current demand for female calves has grown rapidly and controlling the sex of offspring provides an economically flexible management for the livestock producer. The only functioning method of efficiently producing separate populations of X and Y sperm in mammals is based on relative DNA differentiation by high‐speed flow cytometry. In this context, gold nanoparticles conjugated to sex chromosome‐specific moieties display promising application as novel fluorophor‐alternative for the high‐throughput screening, since they feature no photo bleaching, high quantum yield, good biocompatibility and the possibility of non‐destructive membrane penetration. Especially, gold nanoparticles fabricated by pulsed laser ablation are in the recent focus of interest, due to excellent biocompatibility, fabrication‐dependent, tuneable particle size as well as surface charge and ease of (bio)‐functionalization with a remarkably strong ligand binding. For the purpose of our studies functionalized gold nanoparticles may be used as novel markers for sex‐sorting of mammalian sperm and, depending on the selected probe, also for the selection of sperm with heritable DNA‐sequences interesting for animal breeding.

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Section: Nano-revolution Of Genetics and Reproductive Biologymentioning

confidence: 99%

Golden Perspective: Application of Laser‐Generated Gold Nanoparticle Conjugates in Reproductive Biology

Barchanski

Taylor

Klein

et al. 2011

Reprod Domestic Animals

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“…Staining index is a useful metric detector for normalized fluorescent signal over background. 21 In this study, quantification of staining index for fluorophores clearly showed that QD525 exhibited very low background and hence considerably higher staining index compared to FITC.…”

Section: Discussionmentioning

confidence: 56%

Quantum Dot-labeled Tags Improve Minimal Detection Limit of CA125 in Ovarian Cancer Cells and Tissues

Shojaeian¹,

Allameh²,

Jeddi‐Tehrani³

et al. 2018

IJAAI

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In recent years, a lot of attention has been paid to quantum dot (QD) nanoparticles as fluorescent sensors for sensitive and accurate detection of cancer biomarkers. Here, using a homemade specific monoclonal antibody against CA125 and QD525- or FITC-labeled probes, expression of this marker in an ovarian cancer cell line and cancer tissues were traced and optical properties of fluorophores were compared qualitatively and quantitatively. Our results clearly showed that besides lower background and exceptionally higher photobleaching resistance, QD525 exhibited higher fluorescent intensity for both ovarian cancer cell and tissues at different exposure times (p<0.0001) and excitation filter sets (p<0.0001) exemplified by significantly higher staining index (p<0.016). More importantly, the FITC-labeled probe detected antigen-antibody complex at minimum concentration of 0.3 mg/mL of anti-CA125, while reactivity limit decreased to 0.078 mg/mL of anti-CA125 when QD525-labeled probe was applied showing four times higher reactivity level of QD525 probe compared to the same probe labeled with FITC. Based on our results, it seems that QDs are inimitable tags for sensitive detection and localization of ovarian cancer micrometastasis and molecular demarcation of cancer tissues in surgical practice, which subsequently figure out accurate therapeutic approaches.

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“…state that some DC failed to perform as expected regarding signal intensity and binding specificity in FC and MC . Practically, semiconductor quantum dot nanoparticles (Qdots) also function as DCs, as they are detectable by FC according to their fluorescence properties, and likewise by MC, according to their cadmium content .…”

Section: Discussionmentioning

confidence: 94%

Dual‐labelled antibodies for flow and mass cytometry: A new tool for cross‐platform comparison and enrichment of target cells for mass cytometry

et al. 2017

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Antibody conjugates applicable in both conventional flow and mass cytometry would offer interesting options for cross-platform comparison, as well as the enrichment of rare target cells by conventional flow cytometry (FC) sorting prior to deep phenotyping by mass cytometry (MC). Here, we introduce a simple method to generate dual fluorochrome/metal-labelled antibodies by consecutive orthogonal labelling. First, we compared different fluorochrome-conjugated antibodies specific for CD4, such as FITC, Vio667, VioGreen or VioBlue for their compatibility with the conventional secondary MAXPAR labelling protocol. After labelling with Pr, the fluorescence emission spectra of all fluorochromes investigated retained their characteristics, and CD4 dual conjugates (DCs) provided consistent results in immune phenotyping assays performed by FC and MC. The phenotypical composition of CD4 T-cells was maintained after enrichment by FC sorting using different CD4 DCs. Finally, magnetic cell depletion was combined with FC sorting using CD19-VioBlue- Nd, CD20-VioGreen- Sm, CD27-Cy5- Er and CD38-Alexa488- Nd DC to enrich rare human plasmablasts to purities >80%, which allowed a subsequent deep phenotyping by MC. In conclusion, DCs have been successfully established for direct assay comparison between FC and MC, and help to minimise MC data acquisition time for deep phenotyping of rare cell subsets.

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Quantum Dots in Flow Cytometry

Cited by 6 publications

References 20 publications

Golden Perspective: Application of Laser‐Generated Gold Nanoparticle Conjugates in Reproductive Biology

Golden Perspective: Application of Laser‐Generated Gold Nanoparticle Conjugates in Reproductive Biology

Quantum Dot-labeled Tags Improve Minimal Detection Limit of CA125 in Ovarian Cancer Cells and Tissues

Dual‐labelled antibodies for flow and mass cytometry: A new tool for cross‐platform comparison and enrichment of target cells for mass cytometry

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