Quantum dots-labeled polymeric scaffolds for in vivo tracking of degradation and tissue formation

Sim, Kun Hee; Mir, S.M.; Jelke, Sophia; Tarafder, Solaiman; Kim, Jinho; Lee, Chang H.

doi:10.1016/j.bioactmat.2022.03.003

Cited by 4 publications

(3 citation statements)

References 36 publications

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“…We recently developed an imaging-based technology to track in vivo degradation of 3D-printed polycaprolactone scaffolds. 44 Quantum dot labeling enabled quantitative visualization of the remaining portion of the scaffold in vivo without sacrificing animals. We plan to apply this in vivo tracking method to determine the optimal degradation rate of our bioactive scaffold in the next step of translational studies.…”

Section: Discussionmentioning

confidence: 99%

Bioactive Scaffold With Spatially Embedded Growth Factors Promotes Bone-to-Tendon Interface Healing of Chronic Rotator Cuff Tear in Rabbit Model

Han

Kim

et al. 2023

Am J Sports Med

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Background: Functional restoration of the bone-to-tendon interface (BTI) after rotator cuff repair is a challenge. Therefore, numerous biocompatible biomaterials for promoting BTI healing have been investigated. Purpose: To determine the efficacy of scaffolds with spatiotemporal delivery of growth factors (GFs) to accelerate BTI healing after rotator cuff repair. Study Design: Controlled laboratory study. Methods: An advanced 3-dimensional printing technique was used to fabricate bioactive scaffolds with spatiotemporal delivery of multiple GFs targeting the tendon, fibrocartilage, and bone regions. In total, 50 rabbits were used: 2 nonoperated controls and 48 rabbits with induced chronic rotator cuff tears (RCTs). The animals with RCTs were divided into 3 groups: (A) saline injection, (B) scaffold without GF, and (C) scaffold with GF. To induce chronic models, RCTs were left unrepaired for 6 weeks; then, surgical repairs with or without bioactive scaffolds were performed. For groups B and C, each scaffold was implanted between the bony footprint and the supraspinatus tendon. Four weeks after repair, quantitative real-time polymerase chain reaction and immunofluorescence analyses were performed to evaluate early signs of regenerative healing. Histological, biomechanical, and micro–computed tomography analyses were performed 12 weeks after repair. Results: Group C had the highest mRNA expression of collagen type I alpha 1, collagen type III alpha 1, and aggrecan. Immunofluorescence analysis showed the formation of an aggrecan+/collagen II+ fibrocartilaginous matrix at the BTI when repaired with scaffold with GFs. Histologic analysis revealed greater collagen fiber continuity, denser collagen fibers, and a more mature tendon-to-bone junction in GF-embedded scaffolds than those in the other groups. Group C demonstrated the highest load-to-failure ratio, and modulus mapping showed that the distribution of the micromechanical properties of the BTI repaired with GF-embedded scaffolds was comparable with that of the native BTI. Micro–computed tomography analysis identified the highest bone mineral density and bone volume/total volume ratio in group C. Conclusion: Bioactive scaffolds with spatially embedded GFs have significant potential to promote the BTI healing of chronic RCTs in a rabbit model. Clinical Relevance: The scaffolds with spatiotemporal delivery of GF may serve as an off-the-shelf biomaterial graft to promote the healing of RCTs.

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Section: Discussionmentioning

confidence: 99%

Bioactive Scaffold With Spatially Embedded Growth Factors Promotes Bone-to-Tendon Interface Healing of Chronic Rotator Cuff Tear in Rabbit Model

Han

Kim

et al. 2023

Am J Sports Med

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“…Noninvasive monitoring of changes in scaffold composition over time is an investigative area full of developmental potential that could overcome the limitations mentioned above. With the progress of technology, a range of inorganic and organic materials has been developed for monitoring the degradation of bone grafts, which will provide further evidence for investigating the fate of implants in vivo (Dong et al, 2017; Jalani et al, 2015; Sim et al, 2022; Zhang et al, 2016).…”

Section: Bone Regeneration Monitoringmentioning

confidence: 99%

NIR light‐facilitated bone tissue engineering

Feng,

Zhou,

2023

WIREs Nanomed Nanobiotechnol

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In the last decades, near‐infrared (NIR) light has attracted considerable attention due to its unique properties and numerous potential applications in bioimaging and disease treatment. Bone tissue engineering for bone regeneration with the help of biomaterials is currently an effective means of treating bone defects. As a controlled light source with deeper tissue penetration, NIR light can provide real‐time feedback of key information on bone regeneration in vivo utilizing fluorescence imaging and be used for bone disease treatment. This review provides a comprehensive overview of NIR light‐facilitated bone tissue engineering, from the introduction of NIR probes as well as NIR light‐responsive materials, and the visualization of bone regeneration to the treatment of bone‐related diseases. Furthermore, the existing challenges and future development directions of NIR light‐based bone tissue engineering are also discussed.This article is categorized under: Diagnostic Tools > In Vivo Nanodiagnostics and Imaging Implantable Materials and Surgical Technologies > Nanotechnology in Tissue Repair and Replacement

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“…Among the several types of nanostructural labeling agents including magnetic nanopartciles, 11,12 carbon nanotubes, 13 quantum dots (QDs), 14,15 and silicon nanoparticles, 16 some well-designed nanoparticles have primarily been tried for biomedical application due to their excellent physico-chemical properties such as specific optical and electronic properties. 17 This finding has led to the evaluation of inorganic nanoprobes for use in labeling and tracking of stem cells bioimaging.…”

Section: Introductionmentioning

confidence: 99%

Effect of size and morphology of functionalized upconversion nanoparticle labeled probe on stem cell absorption

2023

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In order to improve the application of stem cell therapy, it is important to monitor the proliferation and differentiation of transplanted stem cells in real time, high sensitivity and high precision. Upconversion nanoparticles (UCNPs) have been employed as bioimaging agents and delivery vehicles for gene therapeutics in several types of cells. Herein, we fabricated multiple functional upconversion nanoparticles (FUC-NPs) as exogenous contrast agents to investigate the effect of FUC-NPs size on the efficiency of tracking human umbilical cord mesenchymal stem cells (HUCMSCs). We used TEM, DLS, MTT, etc. systematically detected the effects of nanoparticle dose and exposure time on HUCMSCs cytotoxicity. Our data showed that incubation of FUC-NPs, HUCMSCs are able to maintain their viability and differentiation ability. Interestingly, the small size FUC-NPs examined, S-FUCs, had a much higher uptake capability than the other FUC-NPs (B-FUCs, H-FUCs and R-FUCs).The study will aid in UCNPs for the engineering of stem cell application in the future.

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Quantum dots-labeled polymeric scaffolds for in vivo tracking of degradation and tissue formation

Cited by 4 publications

References 36 publications

Bioactive Scaffold With Spatially Embedded Growth Factors Promotes Bone-to-Tendon Interface Healing of Chronic Rotator Cuff Tear in Rabbit Model

Bioactive Scaffold With Spatially Embedded Growth Factors Promotes Bone-to-Tendon Interface Healing of Chronic Rotator Cuff Tear in Rabbit Model

NIR light‐facilitated bone tissue engineering

Effect of size and morphology of functionalized upconversion nanoparticle labeled probe on stem cell absorption

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