Quantum Dots–Ligand Complex as Ratiometric Fluorescent Nanoprobe for Visual and Specific Detection of G-Quadruplex

Jin, Haojun; Liu, Yuqian; Xu, Tao; Qu, Xiaojun; Bian, Feika; Sun, Qingjiang

doi:10.1021/acs.analchem.6b01967

Cited by 13 publications

(5 citation statements)

References 50 publications

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“…Herein, the ratio of the RLS intensities at two wavelengths was used as signal output. Due to the self-referencing capability, such a ratiometric measurement modality can give high detection accuracy by reducing the effects of the fluctuation of some factors, including light source intensity, instrument sensitivity and probe concentration (22,48,49).…”

Section: Resultsmentioning

confidence: 99%

Cationic porphyrins with large side arm substituents as resonance light scattering ratiometric probes for specific recognition of nucleic acid G-quadruplexes

Zhang

Cui

Zhu

et al. 2019

Nucleic Acids Research

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Specific G-quadruplex-probing is crucial for both biological sciences and biosensing applications. Most reported probes are focused on fluorescent or colorimetric recognition of G-quadruplexes. Herein, for the first time, we reported a new specific G-quadruplex-probing technique—resonance light scattering (RLS)-based ratiometric recognition. To achieve the RLS probing of G-quadruplexes in the important physiological pH range of 7.4-6.0, four water soluble cationic porphyrin derivatives, including an unreported octa-cationic porphyrin, with large side arm substituents were synthesized and developed as RLS probes. These RLS probes were demonstrated to work well for ratiometric recognition of G-quadruplexes with high specificity against single- and double-stranded DNAs, including long double-stranded ones. The working mechanism was speculated to be based on the RLS signal changes caused by porphyrin protonation that was promoted by the end-stacking of porphyrins on G-quadruplexes. This work adds an important member in G-quadruplex probe family, thus providing a useful tool for studies on G-quadruplex-related events concerning G-quadruplex formation, destruction and changes in size, shape and aggregation. As a proof-of-concept example of applications, the RLS probes were demonstrated to work well for label-free and sequence-specific sensing of microRNA. This work also provides a simple and useful way for the preparation of cationic porphyrins with high charges.

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Section: Resultsmentioning

confidence: 99%

Cationic porphyrins with large side arm substituents as resonance light scattering ratiometric probes for specific recognition of nucleic acid G-quadruplexes

Zhang

Cui

Zhu

et al. 2019

Nucleic Acids Research

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“…The ratiometric sensing of molecules that are usually responsible for causing human diseases has always been preferred for a more quantitative and accurate analysis in comparison to the change in intensity of luminescence at a particular wavelength. ,− In this regard, luminscent color- and chromaticity-based sensing, with changes in the blinking characteristics of a single WLE QDC (composed of an inorganic complex and Qdot) following the selective interaction of an analyte with any of the species present in the QDC opens up a new opportunity for the ultrasenstive detection of important molecules . For example, the interaction of the neurotransmitter dopamine with the ZnO Qdot, present in WLE QDC, consisting of a blue-emitting surface Zn(MSA) 2 complex (MSA = N -methylsalicylaldimine), led to changes in the visual emission color from white to blue and chromaticity color coordinates from (0.31, 0.33) to (0.24, 0.23) .…”

Section: Introductionmentioning

confidence: 99%

Chemical Reactions Involving the Surface of Metal Chalcogenide Quantum Dots

et al. 2019

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This invited feature article focuses on the chemical reactions involving the surface ions of colloidal quantum dots (Qdots). Emphasis is placed on ion-exchange, redox, and complexation reactions. The pursuit of reactions involving primarily the cations on the surface results in changes in the optical properties of the Qdots and also may confer new properties owing to the newly formed surface species. For example, the cation-exchange reaction, leading to systematic removal of the cations present on the as-synthesized Qdots, enhances the photoluminescence quantum yield. On the other hand, redox reactions, involving the dopant cations in the Qdots, could not only modulate the photoluminescence quantum yield but also give rise to new emission not present in the as-synthesized Qdots. Importantly, the cations present on the surface could be made to react with external organic ligands to form inorganic complexes, thus providing a new species defined as the quantum dot complex (QDC). In the QDC, the properties of Qdots and the inorganic complex are not only present but also enhanced. Furthermore, by varying reaction conditions such as the concentrations of the species and using a mixture of ligands, the properties could be further tuned and multifunctionalization of the Qdot could be achieved. Thus, chemical, magnetic, and optical properties could be simultaneously conferred on the same Qdot. This has helped in externally controlled bioimaging, white light generation involving individual quantum dots, and highly sensitive molecular sensing. Understanding the species (i.e., the newly formed inorganic complex) on the surface of the Qdot and its chemical reactivity provide unique options for futuristic technological applications involving a combination of an inorganic complex and a Qdot.

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“…Recently, fluorescence-based methods have displayed an obvious advantage in tracing substances because of their high sensitivity, simplicity, cost-effective instrumentation, and direct monitoring in vivo. ,− Most fluorescent probes use the change of intensity at a single emission peak, but it could be influenced by a variety of factors, such as the fluctuation of excitation light intensity, independent of the probe concentration and environmental interference. , Conversely, the ratiometric fluorescent probes based on the ratio of the intensities of two different emission peaks are less prone to suffer the above problems, thus an improved accuracy and sensitivity of the quantification was obtained . In addition, the ratiometric fluorescent method can achieve visual detection of the analyte on account of the various ratios of two fluorescence intensities for simpler and more precise measurements by the naked eye .…”

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confidence: 99%

One-Step Synthesis of Rox-DNA Functionalized CdZnTeS Quantum Dots for the Visual Detection of Hydrogen Peroxide and Blood Glucose

et al. 2017

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As the blood glucose concentration is an important clinical parameter of diabetes, the rapid and effective detection of blood glucose is very significant for monitoring and managing diabetes. Here, a facile method to prepare Rox-DNA functionalized CdZnTeS quantum dots (QDs) was developed. The Rox-DNA functionalized CdZnTeS QDs were prepared by a one-pot hydrothermal method through phosphorothioate DNA bound to QDs, which were employed as a ratiometric fluorescent probe for the rapid and sensitive detection of HO and glucose. Compared with the traditional multistep construction of ratiometric fluorescent probes, this presented approach is simpler and more effective without chemical modification and complicated separation. The CdZnTeS QDs with green fluorescence is specifically sensitive to HO, while the red fluorescence of Rox is invariable. HO is the product from the oxidation of glucose catalyzed by glucose oxidase (GOx). Therefore, a facile method to detect HO and glucose with a detection limit of 0.075 μM for HO and 0.042 μM for glucose was developed. In addition, this proposed probe has been employed for the detection of glucose in human serum with a satisfactory result. Moreover, this probe has been used for visual detection, and the health and diabetics can be distinguished by the naked eye. Meanwhile, this nanoprobe is also generalizable and can be extended to the detection of many other HO-mediated analytes.

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Quantum Dots–Ligand Complex as Ratiometric Fluorescent Nanoprobe for Visual and Specific Detection of G-Quadruplex

Cited by 13 publications

References 50 publications

Cationic porphyrins with large side arm substituents as resonance light scattering ratiometric probes for specific recognition of nucleic acid G-quadruplexes

Cationic porphyrins with large side arm substituents as resonance light scattering ratiometric probes for specific recognition of nucleic acid G-quadruplexes

Chemical Reactions Involving the Surface of Metal Chalcogenide Quantum Dots

One-Step Synthesis of Rox-DNA Functionalized CdZnTeS Quantum Dots for the Visual Detection of Hydrogen Peroxide and Blood Glucose

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