Quantum yield of near-infrared bioluminescence with firefly luciferin analog: AkaLumine

Ono, Ryohei; Osawa, Keita; Takahashi, Yutaka; Noguchi, Yuichi; Kitada, Nobuo; Saito-Moriya, Ryohei; Hirano, Takashi; Maki, Shôjirô; Shibata, Keisei; Akiyama, Hidefumi; Kanno, Ken-ichiro; Itabashi, Hideyuki; Hiyama, Miyabi

doi:10.1016/j.jphotochem.2022.114270

Cited by 3 publications

(3 citation statements)

References 20 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Bioluminescence imaging probes have been mainly used for in vivo visualization of ACT immune cells in preclinical research because of their limited imaging depth and complicated preparation. The development of bioluminescence imaging probes with high sensitivity, long emission wavelengths, and low biotoxicity is an important direction for future research [40,41].…”

Section: Bioluminescence Imaging Probesmentioning

confidence: 99%

Optical imaging of in vivo adoptive T‐cell therapy: State of the art and challenges

Gao

et al. 2023

iRADIOLOGY

View full text Add to dashboard Cite

Adoptive T‐cell therapy (ACT), which is an important type of live cell therapy, has achieved unprecedented success in treating hematological malignancies. Recent studies have shown that ACT is also a promising treatment for solid tumors. Visualizing the in vivo fates (distribution, homing, infiltration, proliferation, and exhaustion) of the immune cells used for ACT (ACT immune cells) is of great importance to promote basic research and clinical translation of ACT. Optical imaging techniques, including bioluminescence, fluorescence, and photoacoustic imaging, have the advantages of high sensitivity, high spatiotemporal resolution, minimal exposure to harmful radiation, and simple instrumentation. Recently, various types of optical imaging probes, including bioluminescence, fluorescence, and photoacoustic imaging probes, have been used to visualize ACT immune cells in vivo and evaluate the molecular mechanism, efficacy, and side effects of ACT. In this review, the optical imaging probes and labeling methods that have been used for in vivo visualization of ACT immune cells are summarized, and the opportunities and challenges of using optical imaging to visualize ACT immune cells in vivo are discussed.

show abstract

Section: Bioluminescence Imaging Probesmentioning

confidence: 99%

Optical imaging of in vivo adoptive T‐cell therapy: State of the art and challenges

Gao

et al. 2023

iRADIOLOGY

View full text Add to dashboard Cite

show abstract

“…Ando et al used a multichannel spectrometer whose spectral responsivity was calibrated in conjunction with a geometric treatment to precisely determine light collection efficiency [46]. QY values of firefly luciferin analogues were also reported using the same instrumentation [47].…”

Section: Determination Of Quantum Yield On Luminescence Reactionmentioning

confidence: 99%

Quantitative Analysis of Bioluminescence Optical Signal

et al. 2023

View full text Add to dashboard Cite

Bioluminescence is light emission based on the luciferin–luciferase enzymatic reaction in living organisms. Optical signals from bioluminescence (BL) reactions are available for bioanalysis and bioreporters for gene expression, in vitro, in vivo, and ex vivo bioimaging, immunoassay, and other applications. Although there are numerous bioanalysis methods based on BL signal measurements, the BL signal is measured as a relative value, and not as an absolute value. Recently, some approaches have been established to completely quantify the BL signal, resulting in, for instance, the redetermination of the quantum yield of the BL reaction and counting the photon number of the BL signal at the single-cell level. Reliable and reproducible understanding of biological events in the bioanalysis and bioreporter fields can be achieved by means of standardized absolute optical signal measurements, which is described in an International Organization for Standardization (ISO) document.

show abstract

“…[12][13][14][15][16][17][18][19] The next issue with firefly bioluminescence is elucidating the mechanism behind the changes in firefly bioluminescence intensity (amount of bioluminescence). In recent years, bioluminescence quantum yields have been reported, [8][9][10]20,21 making it possible to discuss the characteristics of bioluminescence in terms of absolute bioluminescence. The factor determining the quantum yield is the reaction probability.…”

Section: Introductionmentioning

confidence: 99%

Experimental and Theoretical Study for Core Excitation of Firefly Luciferin in Carbon K-Edge Spectra

Kudo,

Kumaki,

Nagasaka

et al. 2024

J. Phys. Chem. A

Self Cite

View full text Add to dashboard Cite

Carbon (C) K-edge X-ray absorption spectra for firefly luciferin were measured and assigned using time-dependent density functional theoretical calculations for luciferin anion and dianion to elucidate the effect of hydroxy-group deprotonation. It was found that the C K-edge spectra for luciferin had four characteristic peaks. The effect of deprotonation of the hydroxy group appears in the energy difference of the first and second peaks of these spectra. This energy difference is 1.0 eV at pH 7 and 2.3 eV at pH 10. The deprotonation of the hydroxy group can be distinguished based on the soft X-ray absorption spectra.

show abstract

Quantum yield of near-infrared bioluminescence with firefly luciferin analog: AkaLumine

Cited by 3 publications

References 20 publications

Optical imaging of in vivo adoptive T‐cell therapy: State of the art and challenges

Optical imaging of in vivo adoptive T‐cell therapy: State of the art and challenges

Quantitative Analysis of Bioluminescence Optical Signal

Experimental and Theoretical Study for Core Excitation of Firefly Luciferin in Carbon K-Edge Spectra

Contact Info

Product

Resources

About