Quartz crystal microbalance based histidine sensor

Abstract

Herein, quartz crystal microbalance (QCM) biosensor is prepared for the detection of L-histidine by attachment of L-histidine imprinted poly(EGDMA-MAH/Cu(II)) nanoparticles on QCM electrode. The imprinted nanoparticles with the size of 86.43 nm were synthesized via miniemulsion polymerization reaction. Prepared QCM sensors were characterized with ellipsometer, contact angle measurements and FTIR. The thickness measurements demonstrated that the particle thin films were almost monolayer. L-histidine solutions with a concentration range between 6.44 lM and 225.6 lM were introduced to QCM system to determine the adsorption kinetics. Selectivity of the L-histidine imprinted nanoparticles were examined using D-histidine and L-tryptophan as competitor molecules. L-histidine imprinted QCM biosensors was also used for RNAase, lysozyme, cytochrome-C and BSA to investigate the competitive adsorption of surface histidine exposed proteins.

Recent advances of medical biosensors for clinical applications

“…They prepared sample solutions with a concentration range from 6.44 μM to 225.6 μM and applied to a piezoelectric medical biosensor to obtain the binding kinetics. They also performed the selectivity of the piezoelectric medical biosensor using lysozyme, RNAase, bovine serum albumin and cytochrome‐C to explore the competitive adsorption of surface histidine exposed proteins (Sönmezler et al, 2019). Bakhshpour et al developed a piezoelectric medical biosensor to detect human metastatic breast cancer cells (MDA MB 231).…”

Diclofenac Imprinted Surface Plasmon Resonance (SPR) Based Sensor

“…[35] In recent decades, SPR sensors coupled with molecular imprinted polymeric structures have attracted great attention as biorecognition elements. [36][37][38][39] Molecular imprinting technique enables to form cavities in the crosslinked polymer backbones bearing the ability to identify the shape and size of the analyte. Imprinted cavities can be formed by the appropriate polymerization and these imprinted cavities ensure the selective recognition of template molecule.…”

Development of an Electrochemical Sensor for Selective Determination of Dopamine Based on Molecularly Imprinted Poly(p‐aminothiophenol) Polymeric Film

“…Besides they have resistance to enviromental factors like temperature, very high or low pH, organic solvents and they can easily regenerate themselves [8,9]. Due to these advantages, the usage of molecularly imprinted polymers has become a milestone for detection of biological and chemical molecules like amino acids [10,11], protein molecule [12], drug molecule [13] and also for analysis of food [14]. Chen et al [15] employed a molecularly imprinted potentiometric sensor for chiral analysis of L-phenylalanine.…”