Quartz Crystal Microbalance-Dissipation Technique for Tracking Dynamic Biomacromolecular Interactions

Jackman, Joshua A.

doi:10.1007/5346_2023_34

Cited by 1 publication

(1 citation statement)

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“…Such measurement capabilities have led to its wide usage for characterizing lipid adsorption processes (see also Refs. [46,50] for a detailed discussion of QCM-D merits). Whereas high-quality HLBs behave like non-viscoelastic films with low energy dissipation (hence, negligible ∆D shifts), unruptured lipid vesicles contain high amounts of hydrodynamically coupled solvent and behave like viscoelastic films with high energy dissipation (hence, large ∆D shifts).…”

Section: Resultsmentioning

confidence: 99%

Cholesterol-Enriched Hybrid Lipid Bilayer Formation on Inverse Phosphocholine Lipid-Functionalized Titanium Oxide Surfaces

Sut,

Jackman,

Cho

2023

Biomimetics

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Hybrid lipid bilayers (HLBs) are rugged biomimetic cell membrane interfaces that can form on inorganic surfaces and be designed to contain biologically important components like cholesterol. In general, HLBs are formed by depositing phospholipids on top of a hydrophobic self-assembled monolayer (SAM) composed of one-tail amphiphiles, while recent findings have shown that two-tail amphiphiles such as inverse phosphocholine (CP) lipids can have advantageous properties to promote zwitterionic HLB formation. Herein, we explored the feasibility of fabricating cholesterol-enriched HLBs on CP SAM-functionalized TiO2 surfaces with the solvent exchange and vesicle fusion methods. All stages of the HLB fabrication process were tracked by quartz crystal microbalance-dissipation (QCM-D) measurements and revealed important differences in fabrication outcome depending on the chosen method. With the solvent exchange method, it was possible to fabricate HLBs with well-controlled cholesterol fractions up to ~65 mol% in the upper leaflet as confirmed by a methyl-β-cyclodextrin (MβCD) extraction assay. In marked contrast, the vesicle fusion method was only effective at forming HLBs from precursor vesicles containing up to ~35 mol% cholesterol, but this performance was still superior to past results on hydrophilic SiO2. We discuss the contributing factors to the different efficiencies of the two methods as well as the general utility of two-tail CP SAMs as favorable interfaces to incorporate cholesterol into HLBs. Accordingly, our findings support that the solvent exchange method is a versatile tool to fabricate cholesterol-enriched HLBs on CP SAM-functionalized TiO2 surfaces.

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Section: Resultsmentioning

confidence: 99%