Quasichemical description of the cell death kinetics of cellular biosensor Spirostomum ambigua for testing the biological activity of aqueous solutions

Гончарук, В. В.; Сыроешкин, А. В.; Zlatskiy, I. A.; Uspenskaya, E. V.; Orekhova, Anastasia; Levitskaya, O. V.; Dobrovolskiy, Vladimir; Pleteneva, T. V.

doi:10.3103/s1063455x17020072

Cited by 21 publications

(21 citation statements)

References 13 publications

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“…Optimal lifespan values correlated with the natural D/H ratio in water. It is interesting that in this case Ea does not change, as we noted it earlier [ 19 ].…”

Section: Resultssupporting

confidence: 77%

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Dependence of Biocatalysis on D/H Ratio: Possible Fundamental Differences for High-Level Biological Taxons

et al. 2020

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The kinetics of biological reactions depends on the deuterium/protium (D/H) ratio in water. In this work, we describe the kinetic model of biocatalytic reactions in living organisms depending on the D/H ratio. We show that a change in the lifetime or other characteristics of the vital activity of some organisms in response to a decrease or increase in the content of deuterium in the environment can be a sign of a difference in taxons. For animals—this is a curve with saturation according to the Gauss’s principle, for plants—it is the Poisson dependence, for bacteria a weakly saturated curve with a slight reaction to the deuterium/protium ratio toward increasing deuterium. The biological activity of the aquatic environment with reduced, elevated, and natural concentrations of deuterium is considered. The results of the study are presented in different vital indicators of some taxons: the bacteria kingdom—the colony forming units (CFU) index (Escherichia coli); animals—the activation energy of the death of ciliates (Spirostomum ambiguum), embryogenesis of fish (Brachydanio rerio); plants—germination and accumulation of trace elements Callisia fragrans L., sprouting of gametophores and peptidomics of moss Physcomitrella patens. It was found that many organisms change their metabolism and activity, responding to both high and low concentrations of deuterium in water.

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“…Optimal lifespan values correlated with the natural D/H ratio in water. It is interesting that in this case Ea does not change, as we noted it earlier [ 19 ].…”

Section: Resultssupporting

confidence: 77%

“…The survival values of the unicellular biosensor S.ambiguum show the dependence on the D/H isotopic ratio in water according to the principle of the distribution of the Gaussian curve, taking into account the previously presented data [ 17 , 19 ]. Optimal lifespan values correlated with the natural D/H ratio in water.…”

Section: Resultsmentioning

confidence: 99%

Dependence of Biocatalysis on D/H Ratio: Possible Fundamental Differences for High-Level Biological Taxons

et al. 2020

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“…4). The D/H ratio decrease in water leads to an increase in the lifetime of the ciliate due to the manifestation of the kinetic isotope effect [20][21][22]. But any complication of the system due to the addition of excipients to the aqueous solution leads to a decrease in lifetime leads of the biological object.…”

Section: Aa and Dhaamentioning

confidence: 99%

The the Effect of Thermal Sterilization and Excipients on the Stability of Ascorbic Acid in Aqueous Solutions

Сыроешкин¹,

Pleteneva²,

Uspenskaya³

et al. 2019

Int J App Pharm

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Objective: To investigate the thermal stability of aqueous solutions of L-ascorbic acid (AA) and its reactions with excipients for the improvement of the injection forms technology. Methods: Solutions of L-ascorbic acid were prepared using deuterium depleted water (DDW-«light» water, D/H=4 ppm) and natural deionized high-ohmic water (BD, D/H=140 ppm). The optical rotation was observed using an automatic polarimeter Atago POL-1/2. Electrospray tandem mass spectra were recorded by Sciex X500R QTOF. Electronic spectra were recorded by UV-spectrometer Cary 60 (Agilent). Unicellular biosensor (Spirotox-test) was used for investigation of excipients influence on the AA biological activity. The statistical analysis was carried out using the OriginPro®9 packages. Results: The results demonstrate the thermal instability of AA. The optical activity of injection forms of AA differs from model solutions with the same concentration and pH value but without heat treatment. Monitoring of solutions by the LC-ESI-MS/MS method made it possible to characterize the nature of some thermal decomposition products. Thermodynamic calculations and evaluation of biological activity (Spirotox-test) indicate that AA interacts with sulfite-ion by redox mechanisms. Excipients in AA aqueous solutions decrease, but DDW increases the biological object lifetime. Conclusion: The use of the set of physicochemical and biological methods to study the effect of heat treatment of L-ascorbic acid solutions in the presence of sulfur (IV) compounds as excipients made it possible to identify decomposition products of the active pharmaceutical ingredient. The results indicate the need to exclude sterilization of the AA injection form by the thermal method and replace it with an alternative one, for example, with gamma radiation treatment.

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“…Lowpower daylight lamps (~10 W) were used for lighting. In general, the experimental procedure was consistent with the generally accepted one [14] with some modifications [15].…”

Section: The Determination Of the Biological Activity Of Ps Using Thementioning

confidence: 54%

Mechanochemical Activation of Pharmaceutical Substances as a Factor for Modification of Their Physical, Chemical and Biological Properties

et al. 2019

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Objective: Study the influence of the mechanical preparation methods (grinding, fluidization) of solid pharmaceutical substances (PS) and herbal raw material on their physicochemical properties and biological activities. Methods: Test substances and solvents-Lactose monohydrate (DFE Pharma, Germany). Sodium chloride, bendazol hydrochloride (all Sigma-Aldrich, USA) and herbal raw material (Callisia fragrans). The dispersity and native structure of pharmaceutical substances were analyzed by several methods: optical microscopy–Altami BIO 2 microscope (Russia); low angle laser light scattering (LALLS) method (Malvern Instruments, UK); Spirotox method–Quasichemical kinetic of cell transition of cellular biosensor Spirostomum ambiguum; Fourier-transform infrared spectroscopy–the analysis in the middle IR region was carried out using an IR Cary 630 Fourier spectrometer (Agilent Technologies, USA). The analysis of dried leaves of C. fragrans before and after mechanical activation was performed using Shimadzu EDX-7000 X-ray fluorescence spectrophotometer without mineralization (Shimadzu, Japan). Results: It was established that the mechanical change, such as dispersion and drying, alters the biological activity of PS and herbal raw materials. The observed increase in the influence of the dispersed substance on the biosensor S. ambiguum is quantitatively estimated from the values of the activation energy (obsEa), which turns to be valued 1,5 (P≤0,05) times more than for the native form substance. In the study of the dependence of the availability of chemical elements K, Ca, Zn on the degree of dispersion of herbal raw materials was established a quantitative 4-fold (P≤0,05) increase in the concentration of elements in mechano-activated raw materials. Conclusion: By the example of the biological model of Spirotox (single-celled biosensor S. ambiguum) and herbal raw materials obtained from C. fragrans, the increase of biological activity of PS at the dispersion of initial preparations was proved.

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Quasichemical description of the cell death kinetics of cellular biosensor Spirostomum ambigua for testing the biological activity of aqueous solutions

Cited by 21 publications

References 13 publications

Dependence of Biocatalysis on D/H Ratio: Possible Fundamental Differences for High-Level Biological Taxons

Dependence of Biocatalysis on D/H Ratio: Possible Fundamental Differences for High-Level Biological Taxons

The the Effect of Thermal Sterilization and Excipients on the Stability of Ascorbic Acid in Aqueous Solutions

Mechanochemical Activation of Pharmaceutical Substances as a Factor for Modification of Their Physical, Chemical and Biological Properties

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