Quasielastic Light Scattering and Cholesterol Thermodynamic Activity Studies of the Micelle-to-Vesicle Transition in Conjugated Ursodeoxycholate-Lecithin-Cholesterol Solutions

Liu, Chen L.; Jain, Uday K.; Higuchi, William I.; Mazer, Norman A.

doi:10.1006/jcis.1994.1059

Cited by 16 publications

(54 citation statements)

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“…According to eq 8, the proportionality constant decreased 50% when C 2 was 3.9 g/dL. Therefore, it appears that the estimated molecular weight of mixed TC-L micelles, based on the technique of an unchanged proportionality constant reported previously, 11,20,22 was within a 50% error. This finding indicates that the previous technique needs to be reevaluated when estimating the molecular weight of particles.…”

Section: Interaction and Molecular Weight Of Mixed Tc-l Micellesmentioning

confidence: 83%

“…3,11 According to the theory of Nagarajan and the fact of nearly constant size of simple TC micelles, a monomermicelle model is confirmed. 22 10 the best values of n and cmc were 4.8 and 10.1 mM from a nonlinear least-squares curvefitting program, SCIENTIST (MicroMath, Salt Lake City, UT). Interestingly, the resulting values are within an acceptable range of n (3-7) and cmc (4-14 mM) reported previously when different techniques were used.…”

Section: Resultsmentioning

confidence: 99%

“…Based on eq 10 and values from Table 2, a good curve fitting was obtained from SCIENTIST (MSC ) 4.48), as shown in Figure 2. The A 22 and M 2 values obtained from the curve fitting were 5.09 × 10 -6 dL mol g -2 and 35 840 Da, as shown in Table 3. Similar to the procedure for the estimation of molecular weight of simple TC micelles, when the hydrodynamic radius of mixed TC-L micelles was ∼24 Å, the molecular weight of mixed TC-L was estimated to be ∼35 000 Da.…”

Section: Interaction and Molecular Weight Of Mixed Tc-l Micellesmentioning

confidence: 99%

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Interactions and Molecular Weights of Simple Micelles and Mixed Micelles in Taurocholate and Taurocholate−Lecithin Solutions

Liu

1997

J. Phys. Chem. B

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A quasielastic light-scattering (QLS) technique was employed to investigate simple micelles and mixed micelles formed in taurocholate (TC) and TC-lecithin (L) solutions. When the size and polydispersity of simple TC micelles were nearly unchanged with total TC concentrations, the simple TC micelles were suggested to be all of a single size. According to the relationship between the total TC concentration and the TC monomer concentration, a monomer-micelle model predicted the aggregation number (n) and the critical micellar concentration (cmc) of simple TC micelles to be 5 and 10 mM. Based on Flory's theory, the scattered intensity, the TC monomer concentration, the molecular weights of simple TC micelles, and mixed TC-L micelles were estimated to be 2138 Da and 35 840 Da. Moreover, the interaction coefficients of simple TC micelles and mixed TC-L micelles were found to be 1.30 × 10 -5 dL mol g -2 and 5.09 × 10 -6 dL mol g -2 . According to Flory's equation, the estimated interaction coefficients and the concentrations of simple TC micelles and mixed TC-L micelles resulted in different values of the proportionality constant. When the proportionality constant values were assumed to be unchanged, significant errors in the determined molecular weights of simple TC micelles and mixed TC-L micelles occurred.

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Section: Interaction and Molecular Weight Of Mixed Tc-l Micellesmentioning

confidence: 83%

Section: Resultsmentioning

confidence: 99%

Section: Interaction and Molecular Weight Of Mixed Tc-l Micellesmentioning

confidence: 99%

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Interactions and Molecular Weights of Simple Micelles and Mixed Micelles in Taurocholate and Taurocholate−Lecithin Solutions

Liu

1997

J. Phys. Chem. B

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“…However, when the total bile salt present is 50% conjugated UDC (i.e., simulated bile of patients under the therapy of conjugated UDC), vesicle formation occurs at a Ch thermodynamic activity ( A T ) of 0.85–0.9, suggesting that the potential for mesophase formation in the therapeutic situation is only marginal 25, 26. Further, based on the total intensity of quasielastic light‐scattering measurements, the vesicles actually represented only a small fraction of the total lipid concentration in conjugated UDC:L:Ch solutions, although they dominated the scattered intensity 26. Eckhardt et al27 also reported that Ch was solubilized mainly in mixed micelles of Ch gallstone patient biles.…”

Section: Introductionmentioning

confidence: 99%

“…Due to overestimation of the contribution of vesicles in the solubilization of Ch, it is essential to investigate the roles of simple micelles and mixed micelles in model bile rich in conjugated UDC. Nevertheless, previous studies have shown that, from a thermodynamic standpoint, simple micelles solubilize only a small fraction of the total Ch solubilization in bile salt‐lecithin micellar solutions 10, 12, 25, 26…”

Section: Introductionmentioning

confidence: 99%

Estimation of Cholesterol Solubilization by a Mixed Micelle Binding Model in Aqueous Tauroursodeoxycholate:Lecithin:Cholesterol Solutions

Higuchi

Tzeng

Chang

et al. 2008

Journal of Pharmaceutical Sciences

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Estimation of the Taurocholate to Lecithin Molar Ratio of Mixed Micelles in Taurocholate‐Lecithin Solutions by an Interaction Model and Laser Light‐Scattering Measurements

Liu

Weng

2003

J Chinese Chemical Soc

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The taurocholate (TC) to lecithin (L) molar ratio (K) and molecular weight of mixed TC-L micelles coexisting with simple TC micelles were determined by employing laser light-scattering measurement and an interaction model of mixed and simple micelles. When the nonmixed micellar taurocholate (NMTC) concentration was greater than 25 mM, the K value and molecular weight of mixed TC-L micelles were~4.3 and2 0,000 daltons, respectively, at an L concentration of 6 mM, and~2.0 and~35,000 daltons at L concentrations of 12 mM and 21 mM. A quantitative model of the formation of simple TC micelles and mixed TC-L micelles indicated that, when the NMTC concentration was greater than 25 mM, mixed TC-L micelles were unstable at low L levels because of low concentrations of L monomers.

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Quasielastic Light Scattering and Cholesterol Thermodynamic Activity Studies of the Micelle-to-Vesicle Transition in Conjugated Ursodeoxycholate-Lecithin-Cholesterol Solutions

Cited by 16 publications

References 0 publications

Interactions and Molecular Weights of Simple Micelles and Mixed Micelles in Taurocholate and Taurocholate−Lecithin Solutions

Interactions and Molecular Weights of Simple Micelles and Mixed Micelles in Taurocholate and Taurocholate−Lecithin Solutions

Estimation of Cholesterol Solubilization by a Mixed Micelle Binding Model in Aqueous Tauroursodeoxycholate:Lecithin:Cholesterol Solutions

Estimation of the Taurocholate to Lecithin Molar Ratio of Mixed Micelles in Taurocholate‐Lecithin Solutions by an Interaction Model and Laser Light‐Scattering Measurements

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