The hydrogen isotopic composition (δD) of leaf wax long-chain n-alkanes (C 27 , C 29 , and C 31 ) from lacustrine sediments has been widely applied to reconstruct terrestrial paleoclimatic and paleohydrological changes. However, few studies have addressed whether the aquatic-derived n-alkanes can affect the δD values of lake sedimentary long-chain n-alkanes, which are usually regarded as a recorder of the terrestrial hydrological signals. Here we systematically investigated δD values of long-chain n-alkanes from modern aquatic plants, both near-shore and off-shore surface sediments, surrounding terrestrial plant litters, as well as river water and lake water in Lake Qinghai and its satellite lakes on the northeastern Qinghai-Tibet Plateau. Our data showed that (i) δD values of long-chain n-alkanes from aquatic plants varied from 184‰ to 132‰ for n-C 27 , from 183‰ to 138‰ for n-C 29 , and from 189‰ to 130‰ for n-C 31 , respectively, with no significant differences among the three n-alkanes homologues; (ii) δD values of long-chain n-alkanes from aquatic plants were generally more positive than those from surrounding terrestrial plants, possibly because that they recorded the D-enrichment of lake water in this semi-arid region; (iii) δD values of long-chain n-alkanes from surface sediments showed significant differences among the three n-alkanes homologues, due to the larger aquatic input of n-C 27 to the sedimentary lipid pool than that of n-C 31 , and (iv) n-C 27 δD values of near-shore aquatic plants and near-shore sediments are more negative than those from off-shore as a result of lower δD values of near-shore lake water. Our findings indicate that in this region (i) the offset between sedimentary n-C 27 and n-C 31 δD values (∆δD C27-C31 ) could potentially be used to evaluate if sedimentary long-chain n-alkanes are derived from a single source; (ii) while δD values of n-C 27 may be influenced by lake water hydrological changes, sedimentary n-C 31 is derived predominantly from terrestrial plants and thus its δD can serve as a relatively reliable indicator for terrestrial paleoclimatic and paleohydrological reconstructions.