behavior in the neighborhood of defects and their dynamics. [4] Disclination lines provide a topological stability that can be instrumental for new switching modes, to realize bistable switching, etc. Studying the formation, interaction, and responsiveness of LC disclinations is therefore not only interesting from a theoretical point of view but also opens up new possibilities for technological applications.Disclination lines can connect defects defined at the surface or can form closed loops in the bulk of a NLC, for example when encircling colloidal inclusions, [5] or when rapid but continuous variations in the alignment direction occur independently at opposing substrates of a device. [1d,e,h,i,3c] To realize patterned surface anchoring, scribing, microrubbing, surface topography, pillars, and photoalignment have been used. [1,2] In the case of strong anchoring, a defect in the alignment pattern with charge s generates 2|s| disclination lines in the bulk of the LC. In the case of weak anchoring and gliding, defects can become mobile and disclinations lines may connect two defects of the same charge on opposing substrates. [6] Thanks to photoalignment, stable defects can now easily be realized experimentally, as has been demonstrated in a few papers. [1d,e,3] The flexibility of photoalignment patterning in combination with the stimuli-responsiveness (e.g., electrical tunability) of LCs allows to design disclination networks with preprogrammed functionality. [2b,3c] Enhanced understanding of the LC behavior in cells with 2D patterns of topological defects is however still necessary to successfully engineer functional LC devices such as multistable photonic components, programmable origami or devices with directed self-assembly of colloidal particles. To study the interaction of NLC disclination lines and their behavior under applied voltages, we defined a square grid pattern of ½ defects on the bottom substrate in combination with a uniformly planar aligned top substrate. Different types of interconnections occur between the +1/2 and −1/2 surface defects, and the three most common types of bulk disclinations are analyzed in detail by comparing experiments with numerical simulations (with and without applied voltage). In the discussion section also a theoretical analysis is provided, explaining the curvature of the different disclination lines and including the effect of unequal elastic constants for splay, twist, and bend. To reduce the splay energy, a tilt of the director is induced close to the +1/2 surface defect. We managed to make this visible in the microscope by increasing the tilt under influence of an electric field.Photoalignment for nematic liquid crystals makes it possible to design complex alignment patterns with point defects, that can act as anchoring points for disclination lines. This feature may be used to realize novel electro-optical devices with bistability or enhanced scattering and is promising for material applications such as stimuli-responsive actuators, active matter, and assemb...