Quaternized chitosan particles as ion exchange supports for label-free DNA detection using PNA probe and MALDI-TOF mass spectrometry

Meebungpraw, Jittima; Wiarachai, Oraphan; Vilaivan, Tirayut; Kiatkamjornwong, Suda; Hoven, Voravee P.

doi:10.1016/j.carbpol.2015.05.043

Cited by 10 publications

(3 citation statements)

References 35 publications

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“…270 The differential electrostatic properties of PNA and DNA have been utilized for the detection of the DNA sequence. According to this principle, the negatively charged DNA was selectively captured via electrostatic interactions on various surfaces such as polymer beads, 271,272 magnetic beads, 273 cellulose paper, 274 or carbon electrode 275 that were coated with a positively charged polymer such as chitosan or poly(2-(dimethylamino)ethyl methacrylate) (PQDMAEMA). The neutral PNA molecule could not interact with such a surface unless it was hybridized with the captured DNA target, and the presence of the PNA probe hybridized to DNA could be detected by optical, electrochemical, or mass spectrometric detection.…”

Section: Modified Pna For Nucleic Acid Biosensing and Bioimagingmentioning

confidence: 99%

Perspectives on conformationally constrained peptide nucleic acid (PNA): insights into the structural design, properties and applications

Suparpprom

Vilaivan

2022

RSC Chem. Biol.

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Section: Modified Pna For Nucleic Acid Biosensing and Bioimagingmentioning

confidence: 99%

Perspectives on conformationally constrained peptide nucleic acid (PNA): insights into the structural design, properties and applications

Suparpprom

Vilaivan

2022

RSC Chem. Biol.

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“…Since PNA don't contain negative charge, there is no electrostatic repulsion between PNA and DNA or RNA, so the stability and sensitivity of hybridization are greatly improved, and it has good ability to recognize specific sequences. These properties make PNA one of the most effective tools for biomedical applications [20][21][22]. In this study, PNA probe was used to replace oligonucleotide probe for the preparation of the LFB.…”

Section: Introductionmentioning

confidence: 99%

Lateral Flow Biosensor for microRNA Detection Based on Peptide Nucleic Acid Probes

Liu¹,

Xia²,

Huang³

et al. 2023

Preprint

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Malignancy is a fatal disease with an increasing incidence with each passing year, and the early diagnosis of malignancy can significantly improve the cure rate of patients, which has profound implications for saving patients' lives. The changes of miRNA are closely related to the occurrence of malignant tumors. The expression of miRNA in many malignant tumors is obviously different and unique from that in normal tissues. In this study, a strategy for amplifying microRNA (miRNA) signal was designed using duplex specific nuclease (DSN), which was then combined with Peptide nucleic acid (PNA) probe hybridization technology to prepare a sensitive and efficient paper-based lateral flow biosensor (LFB) for the detection of pancreatic cancer (PC) associated microRNA-10b. The performance evaluation results of the LFB showed that the detection limit of miR-NA-10b is 5 nM/mL, and carries demonstrated well specificity, stability, reproducibility. The biosensor prepared in this paper can provide accurate and rapid detection of miRNA-10b. The detection results play a guiding role in the early diagnosis of PC. Meeting the clinical need for largescale screening of individuals at high risk of PC. Providing new avenues to achieve early mass screening in individuals at high risk of PC.

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“…Here, chitosan membranes were chosen as a solid support for attaching TMPyP for water disinfection because of their properties such as easy fabrication, presence of numerous reactive groups, rigid Dglucosamine structure, and lack of toxic reactions. Chitosan is also biodegradable, cheap and readily available (Crini and Badot, 2008, Amornchai et al, 2004, Krajewska et al, 1990, Martel et al, 2001, Meebungpraw et al, 2015.…”

Section: Introductionmentioning

confidence: 99%

TMPyP functionalised chitosan membrane for efficient sunlight driven water disinfection

Majiya

Chowdhury

Stonehouse

et al. 2019

Journal of Water Process Engineering

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ReuseThis article is distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs (CC BY-NC-ND) licence. This licence only allows you to download this work and share it with others as long as you credit the authors, but you can't change the article in any way or use it commercially. More information and the full terms of the licence here: https://creativecommons.org/licenses/ TakedownIf you consider content in White Rose Research Online to be in breach of UK law, please notify us by emailing eprints@whiterose.ac.uk including the URL of the record and the reason for the withdrawal request. Abstract word count: 237 wordsImportance word count: Text word counts: 5926 words 2 Abstract Sunlight-driven water disinfection system could help provide clean water to some of the I chitosan membranes were modified by pyromellitic dianhydride in order to introduce carboxyl groups and facilitate adsorption of the highly basic photosensitiser -5, 10, 15, 20-tetrakis (1-methyl-4-pyridinio) porphyrin tetra p-toluene sulfonate (TMPyP). The physico-chemical properties of these modified membranes were investigated by microscopy, absorption spectroscopy and Midland surface blotting approaches. The chitosan membrane-TMPyP composite showed photodynamic inactivation of bacteriophage MS2 and E. coli BL21. For photodynamic inactivation under stationary conditions, complete inactivation of MS2 was observed after 90 min illumination at a light intensity of 32 mW cm -2 (which equates to around 3% of bright mid-day time sunlight under clear sky conditions in sub-Saharan Africa). For a flowing system, complete inactivation of MS2 was observed for sample flowing at 0.33 ml/min and passed over the surface of the modified membranes while being illuminated. Reduction of approximately 3 log PFU/ml were observed for samples passed just once over the membranes under the same conditions. With E. coli, under the same stationary conditions, a reduction of 3 log CFU/ml was observed. EachTMPyP functionalised chitosan membrane was used at least three times for the photodynamic inactivation of both MS2 and E. coli BL21 without any detectable loss of inactivation capacity. The re-usability of the membranes will reduce cost and increase the advantage of using an environmental-friendly technology for water disinfection.

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Quaternized chitosan particles as ion exchange supports for label-free DNA detection using PNA probe and MALDI-TOF mass spectrometry

Cited by 10 publications

References 35 publications

Perspectives on conformationally constrained peptide nucleic acid (PNA): insights into the structural design, properties and applications

Perspectives on conformationally constrained peptide nucleic acid (PNA): insights into the structural design, properties and applications

Lateral Flow Biosensor for microRNA Detection Based on Peptide Nucleic Acid Probes

TMPyP functionalised chitosan membrane for efficient sunlight driven water disinfection

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