Quenching-free chemiluminescence method for ranking the antioxidants and inhibition of AAPH-induced membrane peroxidation of red blood cells using new aminophenol derivative

Kinetic Potentiometry as a Method for Studying the Interactions of Antioxidants with Peroxyl Radicals

“…The effectiveness of an inhibitor using thermodynamic parameters, such as antiradical capacity and inhibition coefficient, can be estimated based on the induction period [ 15 , 16 ]. Determining the thermodynamic parameters of the interaction of antioxidants with free radicals is the most common method for studying antioxidant properties [ 9 , 10 , 11 , 12 , 14 , 15 ], both for stable radicals and for radical generating systems. However, traditionally, to study the action of biologically active compounds, both methods for studying pharmacodynamics and pharmacokinetics are used [ 18 , 24 ].…”

“…The AAPH (2,2′-azobis-(2-amidinopropane) hydrochloride) is the most common radical generating system. Peroxyl radicals are formed during the thermal decomposition of AAPH [ 7 , 8 , 9 , 10 , 11 ]. The use of radical generating models is quite common in the study of compounds with antioxidant/inhibitory properties.…”

“…Secondly, the generation of radicals occurs in time at a certain rate, which is also similar to the radicals of the body, in contrast to model systems with stable radicals [ 7 , 8 ]. The fluorescent, the chemiluminescent, and the spectrophotometric methods are used to assess the inhibitory effect of antioxidants in radical generating systems [ 9 , 10 , 11 , 12 , 13 , 14 ]. A multi-stage chemical process is implemented in the chemiluminescent and the fluorescent assays, which, in addition to the main reaction of inhibition of free radical processes, includes activation–deactivation reactions of the compounds used [ 11 , 12 , 13 , 14 ].…”

“…The reaction between generated peroxyl radicals and inhibitors/antioxidants is based on the mechanism of hydrogen atom transfer (HAT-based assays) [ 8 , 9 , 10 ], which in aqueous media can be considered as proton transfer accompanied by electron transfer. In this regard, it is promising to use simple reliable electrochemical methods to study such systems, although their use for radical generating systems is practically not described in the literature.…”

Determinação Da Capacidade Antioxidante Total Baseada No Consumo De Radicais Livres Gerados Por Aaph

“…: linalol) presentes em alimentos e cosméticos (odorizantes). Os parâmetros para a inibição da peroxidação de membranas dos glóbulos vermelhos induzidas por AAPH foram estudadas em 2017 (ZARGOOSH et al, 2017). A atividade antioxidante e a afinidade por água foram avaliadas utilizando como fluoróforo o composto dietil-2-(ciclo-hexilamino)-5-[(E)-2-fenil-1etenil]-3,4-furandicarboxilato.…”