Quercetin-3-O-β-d-glucuronide isolated from Polygonum aviculare inhibits cellular senescence in human primary cells

Abstract

Cellular senescence is known to contribute to tissue aging, a variety of age-related diseases, tissue regeneration, and cancer. Therefore, aging intervention might be useful for prevention of aging as well as age-related disease. In this study, we investigated compounds from Polygonum aviculare to determine if they inhibited cellular senescence in human primary cells, human dermal fibroblasts (HDFs) and human umbilical vein endothelial cells (HUVECs). Ten compounds from P. aviculare were purified and their inhibitory effects on adriamycin-induced cellular senescence were measured by observing senescence-associated β-galactosidase (SA-β-gal) activity and reactive oxygen species. Among them, compound 9 (quercetin-3-O-β-D-glucuronide) showed inhibitory effects against cellular senescence in HDFs and HUVECs treated with adriamycin. Additionally, compound 9 rescued replicative senescence in HDFs and HUVECs. These data imply that compound 9 represses cellular senescence in human primary cells and might be useful for the development of dietary supplements or cosmetics that ameliorate tissue aging or aging-associated diseases.

Effect of quercetin glucosides from Allium extracts on HepG2, PC‑3 and HT‑29 cancer cell lines

“…contribute to the variations in the anticancer properties of the extracts. Previous studies revealed the anticancer effects of quercetin glucosides in in vitro studies (15,20,21). The present results indicate that quercetin glucosides are potent in HepG2, HT-29 and PC-3 cancer cell lines.…”

“…The major extractable quercetin glucosides in the Allium cepa L. were 3,4'-Qdg and 4'-Qmg, and 3'-Qmg was also detected in this species. The composition analysis of Allium cepa L is presented in Table I, which indicates that 3,4'-Qdg is the major phenolic compound present in onions (20,21). Certain quercetin glucosides may be cross-linked to cell-wall polysaccharides in the form of dimers that may be attributed to the quercetin glucosides level in the fraction obtained from different genus Allium (20,21).…”

“…Quercetin glucosides are considered to act as antioxidants in the genus Allium (22,23). The combination of quercetin glucosides may contribute to the anti-proliferative activity of onion extracts on cancer cells (20)(21)(22)(23). Among the quercetin glucosides present in onions, 4'-Qmg has been demonstrated to exhibit the most antioxidant activity in cancer cell lines (23).…”

“…Previous studies have revealed that onions contain an abundant amount of flavonol glycosides as quercetin derivatives (9,17). Flavonoids have been used as a diuretic agent and anti-inflammatory in folk medicine and certain flavonoid-rich types of onion exhibit anticancer properties (18)(19)(20). However, detailed research into the anticancer properties of quercetin has not yet been performed.…”

Quercetin-3-O-β-D-Glucuronide Suppresses Lipopolysaccharide-Induced JNK and ERK Phosphorylation in LPS-Challenged RAW264.7 Cells

“…Recently, it has been reported that avicularin, quercetin-3-α-L-arabinofuranoside, exerts antioxidant and anti-inflammatory activity (Vo et al ., 2012). Quercetin-3- O - β -D-glucuronide (QG) has been reported to anti-cancer (Yamazaki et al ., 2014), anti-aging (Yang et al ., 2014), and anti-oxidant properties (Messer et al ., 2015). However, the underlying mechanism by which QG exerts its pharmacological activity has not been clearly demonstrated.…”