Quercetin acts as an antioxidant and downregulates CYP1A1 and CYP1B1 against DMBA-induced oxidative stress in mice

Abstract

Abstract. We investigated the effects of quercetin on 7,12-dimethylbenz(a)anthracene (DMBA)-induced oxidative stress and the expression of CYP1A1 and CYP1B1 in mice. Quercetin was administered orally to mice at 100 or 250 mg/kg BW for 18 days, after which DMBA (34 mg/kg BW) was administered intragastrically twice. Quercetin showed side effects such as increased aspartate aminotransferase (AST) and alanine aminotransferase (ALT) in DMBA-untreated mice. Also, quercetin induced AST and ALT in DMBA-treated, although this was not significantly different from levels in DMBA-treated controls. The thiobarbituric acid reactive substances (TBARS) value showed a tendency to decrease following quercetin treatment; these decreases were significantly greater in the DMBA-treated compared to the untreated groups. Also, catalase and superoxide dismutase (SOD) activities as well as their mRNA expression were increased by quercetin; this increase was more pronounced in DMBA-treated compared to untreated mice. DMBA induced CYP1 activity as well as expression of CYP1A1 and CYP1B1. Each of these effects was significantly reduced by quercetin; however, this reduction was observed for CYP1A1 at only the higher dose and for CYP1B1 at both doses. These data suggest that quercetin shows antioxidant activity against DMBA-induced oxidative stress. Moreover, its regulation of CYP1A1 and CYP1B1 suggests the potential of quercetin as an anticancer supplement.

Kolaviron pre‐treatment suppresses 7, 12 dimethylbenzanthracene‐induced alterations in estrogen receptor‐α, CYP 1A1, oxidative stress and inflammation in female Wistar rats

“…The results from this study showed significantly higher CYP1A1 activity in DMBAinduced rats that were not pretreated with KV. This finding agrees with a study which showed higher CYP1A1 activity in rodents administered DMBA (Choi et al, 2012). CYP1A1 activity is comparatively higher in breast cancer cells in contrast with healthy breast epithelial cells (Spink et al, 1998).…”

“…CYP1A1 metabolizes various xenobiotics including DMBA and estrogen, resulting in oxidized compounds that contribute to oxidative stress and mammary carcinogenesis (Go et al, 2017). In rodents induced with mammary carcinogenesis using DMBA, increased levels of pro-inflammatory cytokines (Forcados et al, 2020) and elevated levels of oxidative stress markers (Choi et al, 2012) have been reported.…”

PIM1, CYP1B1, and HSPA2 Targeted by Quercetin Play Important Roles in Osteoarthritis Treatment by Achyranthes bidentata

“…We found that CYP1B1 was the target of quercetin, which was consistent with the previous reports. Choi et al reported that quercetin acted as an antioxidant and downregulated CYP1B1 against 7,12-dimethylbenz(a)anthracene-induced oxidative stress in mice [41]. Quercetin showed strong and selective inhibition against CYP1B1, which required 2-3 double bonds on the Cring [42].…”

Temporal Effects of Different Vehicles on Wound Healing Potentials of Quercetin: Biochemical, Molecular, and Histopathological Approaches

“…Corn oil is another vehicle used for the delivery of different drugs including quercetin. 26 In this study, we formulated quercetin in 3 different vehicles, namely, DMSO, ointment base, and corn oil. These 3 formulations of quercetin were comparatively evaluated for the vehicle effects on wound healing potentials of quercetin in rats.…”