Quercetin alleviates generalized hyperalgesia in mice with induced adenomyosis

Abstract

Adenomyosis is a common gynecologic disorder characterized by the presence of endometrial glands and stroma within the myometrium. The present study investigated the effect of quercetin in neonatal Imprinting Control Region mice with tamoxifen-induced adenomyosis. The body weight and hotplate response latency of all mice was examined at 4, 8, 12 and 16 weeks after birth. The mice dosed with tamoxifen were divided into four groups: high- or low-quercetin group, valproic acid (VPA) group and untreated group. The group of mice that were neonatally administrated with the solvent only (no tamoxifen), received no treatment and served as a blank control group. After 3 weeks of drug treatment, the potential ability of quercetin to improve the generalized hyperalgesia in mice with induced adenomyosis was evaluated by determining the body weight, pain modulation, examining the myometrial infiltration by histology examination of the uterus and detecting the expression of transient receptor potential cation channel subfamily V member 1 (Trpv-1), phospho (p)-p38 mitogen activated protein kinase p-extracellular signal-regulated kinase (p-ERK) in DRG neurons via immunohistochemistry. The results demonstrated that treatment with quercetin improved the generalized hyperalgesia by extending the hotplate response latency, reduced myometrial infiltration and decreased the expression levels Trpv-1, p-p38 and p-ERK in dorsal root ganglion neurons. The results indicated that quercetin decreases the incidence of hyperalgesia in mice with tamoxifen-induced adenomyosis, and the potential mechanism is through reduced central sensitization, which may be a promising treatment for adenomyosis.

<p>Quercetin Inhibits Adenomyosis by Attenuating Cell Proliferation, Migration and Invasion of Ectopic Endometrial Stromal Cells</p>

“…Moreover, the reduced levels of these proteins are more significant in EESCs compared with EuESCs. Notably, these results are consistent with a recent study 26 showing that oral treatment of quercetin significantly inhibited the infiltration of myometrium induced by tamoxifen in neonatal mice. Up to date, several Chinese herbs such as berberine and tanshinone IIA have been reported to inhibit the growth, invasion and migration through regulating NF-κB/p65, 39 LPS/TLR4, 40 and 14-3-3ζ signaling pathways.…”

“…Our findings are not in accordance with a previous study based on animal models. 26 Specifically, Nie et al 26 demonstrated that intragastric administration of quercetin caused no significant body weight loss in neonatal Imprinting Control Region (ICR) mice induced with adenomyosis compared with the untreated control group. However, this result found by Nie et al may be due to a pain-suppressed behavior and concomitant-reduced food intake.…”

“…However, this result found by Nie et al may be due to a pain-suppressed behavior and concomitant-reduced food intake. 26,27 Thus, whether quercetin could inhibit the EESCs proliferation in adenomyosis in vivo also needs to be investigated in the future.…”

Novel dietary supplement association reduces symptoms in endometriosis patients

“…Therefore, the ability of this | 5923 combination of dietary supplements to decrease PGE2 levels could justify the clinical effects seen in the patients at the end of the treatment. Nevertheless, it must be also considered the recent observation that treatment with quercetin improves the generalized hyperalgesia in an animal model of adenomyosis (Nie & Liu, 2017).…”

“…Quercetin, reduces local estrogen by reducing FSH and LH (animal model studies), and reduces VEGF (Massi et al, 2017). Moreover, it has recently been demonstrated that quercetin decreases the incidence of hyperalgesia in mice with experimental adenomyosis through the reduction of central sensitization and this could be a promising treatment for adenomyosis (Nie & Liu, 2017).…”