Quercetin alleviates Mycoplasma gallisepticum-induced inflammatory damage and oxidative stress through inhibition of TLR2/MyD88/NF-κB pathway in vivo and in vitro

Wang, Shun; Jin, Xiaodi; Chen, Hao; Han, Mingdong; Bao, Jun; Dong, Na; Wang, Yikang; Li, Rui; Wu, Zhiyong; Li, Jichang

doi:10.1016/j.micpath.2023.106006

Cited by 8 publications

(2 citation statements)

References 47 publications

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“…From what has been discussed above, quercetin mediates the AMPK/SIRT1/NF-κB signaling pathway to inhibit the MG-induced inflammatory response (Figure 9). In particular, the findings of our partners also confirm quercetin's ability to resist MG-induced inflammation, an anti-inflammatory mechanism that works by inhibiting the activity of the TLR2/myD88/NF-kB pathway [46]. Quercetin is suggested to achieve multi-target anti-inflammatory pharmacological effects.…”

Section: Discussionsupporting

confidence: 72%

Quercetin and AMPK: A Dynamic Duo in Alleviating MG-Induced Inflammation via the AMPK/SIRT1/NF-κB Pathway

Lu,

Wang,

Ishfaq

et al. 2023

Molecules

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Mycoplasma gallisepticum (MG) is recognized as a principal causative agent of avian chronic respiratory disease, inflicting substantial economic losses upon the poultry industry. However, the extensive use of conventional antibiotics has resulted in the emergence of drug resistance and various challenges in their clinical application. Consequently, there is an urgent need to identify effective therapeutic agents for the prevention and treatment of mycoplasma-induced respiratory disease in avian species. AMP-activated protein kinase (AMPK) holds significant importance as a regulator of cellular energy metabolism and possesses the capacity to exert an anti-inflammatory effect by virtue of its downstream protein, SIRT1. This pathway has shown promise in counteracting the inflammatory responses triggered by pathogenic infections, thus providing a novel target for studying infectious inflammation. Quercetin possesses anti-inflammatory activity and has garnered attention as a potential alternative to antibiotics. However, there exists a gap in knowledge concerning the impact of this activation on MG-induced inflammatory damage. To address this knowledge gap, we employed AlphaFold2 prediction, molecular docking, and kinetic simulation methods to perform a systematic analysis. As expected, we found that both quercetin and the AMPK activator AICAR activate the chicken AMPKγ1 subunit in a similar manner, which was further validated at the cellular level. Our project aims to unravel the underlying mechanisms of quercetin’s action as an agonist of AMPK against the inflammatory damage induced by MG infection. Accordingly, we evaluated the effects of quercetin on the prevention and treatment of air sac injury, lung morphology, immunohistochemistry, AMPK/SIRT1/NF-κB pathway activity, and inflammatory factors in MG-infected chickens. The results confirmed that quercetin effectively inhibits the secretion of pro-inflammatory cytokines such as IL-1β, TNF-α, and IL-6, leading to improved respiratory inflammation injury. Furthermore, quercetin was shown to enhance the levels of phosphorylated AMPK and SIRT1 while reducing the levels of phosphorylated P65 and pro-inflammatory factors. In conclusion, our study identifies the AMPK cascade signaling pathway as a novel cellular mediator responsible for quercetin’s ability to counter MG-induced inflammatory damage. This finding highlights the potential significance of this pathway as an important target for anti-inflammatory drug research in the context of avian respiratory diseases.

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Section: Discussionsupporting

confidence: 72%

Quercetin and AMPK: A Dynamic Duo in Alleviating MG-Induced Inflammation via the AMPK/SIRT1/NF-κB Pathway

Lu,

Wang,

Ishfaq

et al. 2023

Molecules

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“…Chlorogenic acid, extracted from honeysuckle, has been shown to increase the live body weight and reduce pathological damage, making it an alternative therapeutic option for broilers infected with MG (Müştak et al, 2015). Quercetin, an active compound in the traditional herbal medicine Ephedra sinica, has been discovered to effectively inhibit inflammatory damage and oxidative stress caused by MG infection (Wang et al, 2023a). The use of puerarin has been shown to effectively inhibit the inflammatory response and apoptosis induced by MG, thereby protecting the lungs from damage caused by the infection.…”

Section: Drug Prevention and Treatment Planmentioning

confidence: 99%

Mycoplasma galliscepticum: An overview

Ruizhi,

Xi,

Huiqi

et al. 2024

Afr. J. Microbiol. Res.

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The prokaryotic microorganism Mycoplasma gallisepticum (MG) belongs to the phylum Firmicutes, the order Mycoplasmatales, the family Mycoplasmataceae, and the genus Mycoplasma. MG parasitizes the respiratory tract of various poultry species, causing a range of symptoms from indistinct features to tracheitis and air sacculitis. Common signs include respiratory tract infection indicators such as coughing, asthma, a runny nose and rales. Both domestic and international reports underscore MG's transmission and infection capacity, signifying its global importance as a source of poultry infection. Further research is imperative to grasp its impact on both farmed and wild animals. This article comprehensively reviews current research progress on MG, encompassing its biological characteristics, infection traits, pathogenic mechanisms, epidemiology, detection methods, treatment, and control measures. It aims to serve as a reference for comprehensive prevention and control strategies against MG in the future.

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