Quercetin ameliorates lipopolysaccharide-induced neuroinflammation and oxidative stress in adult zebrafish

Singh, Sukhdev; Sahu, Kuleshwar; Kapil, Lakshay; Singh, Charan; Singh, Arti

doi:10.1007/s11033-022-07161-2

Cited by 24 publications

(7 citation statements)

References 54 publications

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“…Guan et al (2021a) found that the serum levels of iron, copper, and calcium increased, while magnesium, zinc, selenium, and cobalt levels significantly reduced in CUMS rats, and quercetin restored the levels of these elements, demonstrating that it could regulate trace elements in the brain. Moreover, the beneficial effects of quercetin could also be observed in other animal models, such as guinea pigs and zebrafish (El-Haroun et al, 2021;Singh et al, 2022). These studies confirmed that quercetin exerted antidepressant effects by regulating the activity of enzymatic and non-enzymatic antioxidant systems.…”

Section: Anti-oxidationsupporting

confidence: 57%

Antidepressant Potential of Quercetin and its Glycoside Derivatives: A Comprehensive Review and Update

et al. 2022

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Depression is a global health problem with growing prevalence rates and serious impacts on the daily life of patients. However, the side effects of currently used antidepressants greatly reduce the compliance of patients. Quercetin is a flavonol present in fruits, vegetables, and Traditional Chinese medicine (TCM) that has been proved to have various pharmacological effects such as anti-depressant, anti-cancer, antibacterial, antioxidant, anti-inflammatory, and neuroprotective. This review summarizes the evidence for the pharmacological application of quercetin to treat depression. We clarified the mechanisms of quercetin regulating the levels of neurotransmitters, promoting the regeneration of hippocampal neurons, improving hypothalamic-pituitary-adrenal (HPA) axis dysfunction, and reducing inflammatory states and anti-oxidative stress. We also summarized the antidepressant effects of some quercetin glycoside derivatives to provide a reference for further research and clinical application.

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Section: Anti-oxidationsupporting

confidence: 57%

Antidepressant Potential of Quercetin and its Glycoside Derivatives: A Comprehensive Review and Update

et al. 2022

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“…The outcomes were presented in the format of time spent in the light zone (TSLZ), time spent in the dark zone (TSDZ), and total count of entries in the light zone (TELZ). The videos were monitored and analyzed using video tracking software, specifically ANY-maze …”

Section: Behavioral Parametersmentioning

confidence: 99%

In Vitro and In Vivo Investigations of Chromone Derivatives as Potential Multitarget-Directed Ligands: Cognitive Amelioration Utilizing a Scopolamine-Induced Zebrafish Model

Kumar,

Jangid,

Kumar

et al. 2024

ACS Chem. Neurosci.

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Alzheimer’s disease is a complex neurological disorder linked with multiple pathological hallmarks. The interrelation of therapeutic targets assists in the enhancement of cognitive decline through interference with overall neuronal transmission. We have synthesized and screened various chromone derivatives as potential multitarget-directed ligands for the effective treatment of Alzheimer’s disease. The synthesized compounds exhibited multipotent activity against AChE, BuChE, MAO-B, and amyloid β aggregation. Three potent compounds, i.e., VN-3, VN-14, and VN-19 were identified that displayed remarkable activities against different targets. These compounds displayed IC50 values of 80 nM, 2.52 μM, and 140 nM against the AChE enzyme, respectively, and IC50 values of 2.07 μM, 70 nM, and 450 nM against the MAO-B isoform, respectively. VN-3 displayed potent activity against self-induced Aβ1–42 aggregation with inhibition of 58.3%. In the ROS inhibition studies, the most potent compounds reduced the intracellular ROS levels up to 80% in SH-SY5Y cells at 25 μM concentration. The compounds were found to be neuroprotective and noncytotoxic even at a concentration of 25 μM against SH-SY5Y cells. In silico studies showed that the compounds were nicely accommodated in the active sites of the receptors along with thermodynamically stable orientations. Compound VN-19 exhibited a balanced multitargeting profile against AChE, BuChE, MAO-B, and Aβ1–42 enzymes and was further evaluated for in vivo activities on the scopolamine-induced zebrafish model. VN-19 was found to ameliorate the cognitive decline in zebrafish brains by protecting them against scopolamine-induced neurodegeneration. Thus, VN-3, VN-14, and VN-19 were identified as potent multitarget-directed ligands with a balanced activity profile against different targets and can be developed as therapeutics for AD.

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“…With the use of a microtome, the brain tissues were xed in para n blocks and sectioned into 3 mm thicknesses. Brain segments (5-10 m) were de-waxed and stained with H&E. Under a uorescent microscope, the stained slices were inspected at 40X (27). (Model: 102 M, Motic microscope, China).…”

Section: Histopathological Examinationmentioning

confidence: 99%

“…Following incubation, secondary antibody (polyclonal), enzyme, substrate, and stop reagent were added, followed by washing with wash buffer to remove unbound at various time intervals as speci ed in the procedure. In an ELISA reader, the absorbance was measured at 450nm, and the ndings were expressed in pg/mg protein (27).…”

Section: Tnf-α Il-10 and Il-1βmentioning

confidence: 99%

Ursolic acid along with Caprylic acid Ameliorates Pentylenetetrazole Induced Seizures Like Behavior in Adult Zebrafish

Sharma

Kaur

Kapil

et al. 2022

Preprint

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Background: Epilepsy is a neurological disorder characterized by recurrent unprovoked seizures. Despite the existence of more than 20 anti-epileptic drugs there is still a need for new treatments which could not only treat symptoms but also cure epileptogenesis. PTZ is a Chemoconvulsant that impair GABAergic and glutamatergic neurotransmission, promoting excitotoxicity and seizures. Therefore PTZ exposure has been considered a suitable protocol to assess seizure-like behaviours. UA is a triterpenoid compound having anti-inflammatory, anti-oxidant and neuro protective action. CA is one of the component of ketogenic diet and it supresses the inflammation. Objective: The objective of the present study was to investigate the effect of combination therapy of UA and CA on seizures, neuronal damage and inflammation induced by PTZ in adult zebrafish.Materials and method: Zebrafishes were pretreated with Diazepam (1.25 mg/kg), UA(50 and 150 mg/kg), CA (60 mg/kg) and combination of UA (50 mg/kg) and CA (60 mg/kg) followed by PTZ (170 mg/kg) i.p administration. Various neurobehavioral, biochemical parameters like lipid peroxidase (LPO), catalase, superoxide dismutase, glutathione-s-transferase (GSH) and acetylcholinesterase (AChEs), molecular parameters such as TNF-α, IL-10, Nrf-2 and IL-1β and mitochondrial parameters like complex I, II, IV and MTT assay, histopathological study were performed to determine the effect of therapy.Result: UA of both doses and CA decreased mean seizure score, mean seizure time . Importantly, combination of UA 50 mg/kg and CA 60 mg/kg attenuated seizure-like behavioral scores, decreased mean seizure time, mean seizure score and reduced the frequency of clonic-like seizures (score 4). Combination of UA 50 mg/kg and CA 60 mg/kg also prevented oxidative stress in PTZ-challenged fish by decreasing lipid peroxidation, acetyl cholinesterase activity and increasing catalase, glutathione-s-transferase and superoxide dismutase levels. Additionally, the combination therapy prevented inflammatory response by declining TNF-α and IL-1β levels and raising IL-10 and Nrf-2 levels. Moreover combination of UA 50 mg/kg and CA 60 mg/kg significantly improved mitochondrial complex I, II and IV activity as well as increase MTT assay. Furthermore, morphology of neuronal cell was prevented in combination of UA 50 mg/kg and CA 60 mg/kg when seen in histopathology. Similarly as observed in DZP group, combination of UA 50 mg/kg and CA 60 mg/kg affect the overall swimming activity of fish, suggesting different mechanisms of action. Collectively, we show that combination of UA 50 mg/kg and CA 60 mg/kg attenuates PTZ-induced seizure-like behaviours, brain oxidative stress, mitochondrial and morphological damage of neuronal cell in zebrafish, suggesting the involvement of antioxidant mechanisms in neuroprotection.Conclusion: The present study shows that combination of UA 50 mg/kg and CA 60 mg/kg ameliorates the seizures completely and have neuroprotective action via their anti-inflammatory, anti-oxidant properties.

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Quercetin ameliorates lipopolysaccharide-induced neuroinflammation and oxidative stress in adult zebrafish

Cited by 24 publications

References 54 publications

Antidepressant Potential of Quercetin and its Glycoside Derivatives: A Comprehensive Review and Update

Antidepressant Potential of Quercetin and its Glycoside Derivatives: A Comprehensive Review and Update

In Vitro and In Vivo Investigations of Chromone Derivatives as Potential Multitarget-Directed Ligands: Cognitive Amelioration Utilizing a Scopolamine-Induced Zebrafish Model

Ursolic acid along with Caprylic acid Ameliorates Pentylenetetrazole Induced Seizures Like Behavior in Adult Zebrafish

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