Quercetin ameliorates renal tubulointerstitial transformation and renal fibrosis by regulating NLRP3 in obstructive nephropathy

Ma, Zhi; Wang, Fuqiang; Wang, Huizhi; Sun, Tao; Sun, Wenlu; Xu, Quan

doi:10.23736/s0026-4806.22.08104-6

Cited by 2 publications

(2 citation statements)

References 7 publications

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“… 68 Ma discovered that quercetin inhibited the activation of NLRP3 inflammatory vesicles to improve tubulointerstitial transformation and RF. 69 Therefore, quercetin may effectively treat kidney diseases. Loss or gain in TGF-β expression is thought to stimulate fibroblast activation and proliferation, playing a key role in the fibrosis associated with various CKDs.…”

Section: Discussionmentioning

confidence: 99%

Network Pharmacology Analysis and Machine-Learning Models Confirmed the Ability of YiShen HuoXue Decoction to Alleviate Renal Fibrosis by Inhibiting Pyroptosis

Feng,

Luo,

et al. 2023

DDDT

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Purpose YiShen HuoXue decoction (YSHXD) is a formulation that has been used clinically for the treatment of renal fibrosis (RF) for many years. We aimed to clarify therapeutic effects of YSHXD against RF and potential pharmacological mechanisms. Materials and Methods We used network pharmacology analysis and machine-learning to screen the core components and core targets of YSHXD against RF, followed by molecular docking and molecular dynamics simulations to confirm the reliability of the results. Finally, we validated the network pharmacology analysis experimentally in HK-2 cells and a rat model of RF established by unilateral ureteral ligation (UUO). Results Quercetin, kaempferol, luteolin, beta-sitosterol, wogonin, stigmasterol, isorhamnetin, baicalein, and dihydrotanshinlactone progesterone were identified as the main active components of YSHXD in the treatment of unilateral ureteral ligation-induced RF, with IL-6, IL1β, TNF, AR, and PTGS2 as core target proteins. Molecular docking and molecular dynamics simulations further confirmed the relationship between compounds and target proteins. The potential molecular mechanism of YSHXD predicted by network pharmacology analysis was confirmed in HK-2 cells and UUO rats. YSHXD downregulated NLRP3, ASC, NF-κBp65, Caspase-1, GSDMD, PTGS2, IL-1β, IL-6, IL-18, TNF-α, α-SMA and upregulated HGF, effectively alleviating the RF process. Conclusion YSHXD exerts important anti-inflammatory and anti-cellular inflammatory necrosis effects by inhibiting the NLRP3/caspase-1/GSDMD-mediated pyroptosis pathway, indicating that YSHXD represents a new strategy and complementary approach to RF therapy.

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Section: Discussionmentioning

confidence: 99%

Network Pharmacology Analysis and Machine-Learning Models Confirmed the Ability of YiShen HuoXue Decoction to Alleviate Renal Fibrosis by Inhibiting Pyroptosis

Feng,

Luo,

et al. 2023

DDDT

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“…TLRs/NF-κB activate the downstream inflammasome NOD-like receptor family pyrin domain containing 3 (NLRP3) (Xue et al, 2019), which promotes the maturation of proinflammatory factors IL-1β and IL-18 by activating Caspase-1 (Liston and Masters, 2017). Multiple studies have shown that the NLRP3 inflammasome and its downstream pyroptosis and inflammation play an important role in the development of RF (Ma et al, 2022) (Ram et al, 2022;Wang et al, 2022). It has been shown that Ophiocordyceps sinensis causes inhibition of mRNA and protein expression of NLRP3 inflammasome and downstream effectors IL-1β and IL-18 in a rat model of DN (Wang et al, 2018).…”

Section: Mechanisms Of Cordyceps and Related Products Against Renal F...mentioning

confidence: 99%

Potential Therapeutic Strategies for Renal Fibrosis: Cordyceps and Related Products

Wang

Dai³

et al. 2022

Front. Pharmacol.

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At present, there is no effective drug for the treatment of renal fibrosis; in particular, a safe and effective treatment for renal fibrosis should be established. Cordyceps has several medical effects, including immunoregulatory, antitumor, anti-inflammatory, and antioxidant effects, and may prevent kidney, liver, and heart diseases. Cordyceps has also been reported to be effective in the treatment of renal fibrosis. In this paper, we review the potential mechanisms of Cordyceps against renal fibrosis, focusing on the effects of Cordyceps on inflammation, oxidative stress, apoptosis, regulation of autophagy, reduction of extracellular matrix deposition, and fibroblast activation. We also discuss relevant published clinical trials and meta-analyses. Available clinical studies support the possibility that Cordyceps and related products provide benefits to patients with chronic kidney diseases as adjuvants to conventional drugs. However, the existing clinical studies are limited by low quality and significant heterogeneity. The use of Cordyceps and related products may be a potential strategy for the treatment of renal fibrosis. Randomized controlled trial studies with good methodological quality, favorable experimental design, and large sample size are needed to evaluate the efficacy and safety of Cordyceps.

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Quercetin ameliorates renal tubulointerstitial transformation and renal fibrosis by regulating NLRP3 in obstructive nephropathy

Cited by 2 publications

References 7 publications

Network Pharmacology Analysis and Machine-Learning Models Confirmed the Ability of YiShen HuoXue Decoction to Alleviate Renal Fibrosis by Inhibiting Pyroptosis

Network Pharmacology Analysis and Machine-Learning Models Confirmed the Ability of YiShen HuoXue Decoction to Alleviate Renal Fibrosis by Inhibiting Pyroptosis

Potential Therapeutic Strategies for Renal Fibrosis: Cordyceps and Related Products

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