Quercetin and epigallocatechin-3-gallate effect on the anisotropy of model membranes with cholesterol

Ionescu, Diana N.; Margină, Denisa; Ilie, Mihaela; Iftime, Adrian; Ganea, Constanţa

doi:10.1016/j.fct.2013.03.007

Cited by 20 publications

(15 citation statements)

References 44 publications

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“… 91 Of direct relevance to the mechanistic assessment of EGCG is that all polyphenols in growth media generate H 2 O 2 . Since most polyphenols (including EGCG) interact with cell membranes, 92 - 94 H 2 O 2 molecules typically leave the extracellular environment, partially diffusing into the cells. Based on these processes, Erlank et al proposed that low concentrations of polyphenols, including EGCG, generate H 2 O 2 at very low concentrations in vivo at the level of arteries and capillaries, inducing cellular adaptation to oxidative stress.…”

Section: Discussionmentioning

confidence: 99%

Does Green Tea Induce Hormesis?

et al. 2020

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Green tea, and its principal constituent (–)-epigallocatechin-3-gallate (EGCG), are commonly shown to induce biphasic concentration/dose responses in a broad range of cell types, including non-tumor cells, and tumor cell lines. The most active area of research dealt with an assessment of neural cells with application to neurodegenerative diseases such as Parkinson’s disease and Alzheimer’s disease cell models, often using preconditioning experimental protocols. The general findings demonstrate EGCG-induced hormetic effects resulting in an enhanced acquired resilience within an adaptive and temporally dependent homeodynamic framework. The biphasic dose responses displayed the typical quantitative features of the hormetic dose response with respect to the amplitude and width of the stimulatory response. These findings provide further evidence for the general occurrence of hormetic dose responses with such responses being independent of the biological model, end point, inducing agent, and mechanism. The biphasic nature of these responses has important implications since it suggests optimal dose ranges for end points of public health and therapeutic applications. These findings indicate the need to assess the entire dose-response continuum in order to better define the nature of the dose response, especially in the low-dose zone where such exposures are common in human populations.

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Section: Discussionmentioning

confidence: 99%

Does Green Tea Induce Hormesis?

et al. 2020

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“…At the same time, glucose-stimulated elevation of ROS and was attenuated by both preparations (Figure 10C). It was demonstrated that polyphenols protect cellular membranes against oxidation due to membrane stiffening induction, which hinders the spreading of newly generated radicals [4,48]. In that mechanism reactions of polyphenols with nonpolar compounds present in the hydrophobic inner membrane layer are implicated [4].…”

Section: Membrane Effect Of V Opulusmentioning

confidence: 99%

Evaluation of Viburnum opulus L. Fruit Phenolics Cytoprotective Potential on Insulinoma MIN6 Cells Relevant for Diabetes Mellitus and Obesity

et al. 2020

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In this study, the influence of guelder rose (Viburnum opulus) fruit fresh juice (FJ) and a phenolic-rich fraction (PRF) isolated from juice on mice insulinoma MIN6 cells activities was investigated. Extracts were able to decrease intracellular oxidative stress at the highest non-cytotoxic concentrations. They induced glucagon-like peptide-1 (GLP-1) secretion in the presence of an elevated glucose concentration, and they inhibited in vitro activity of the dipeptidyl peptidase-4 (DPP4) enzyme. Nonetheless, inhibition of glucose-stimulated insulin secretion was detected, which was accompanied by a decrease of cellular membrane fluidity and hyperpolarization effect. In addition, the increase of free fatty acid uptake and accumulation of lipid droplets in MIN6 cells were observed. Elevated extract concentrations induced cell apoptosis through the intrinsic mitochondrial pathway with activation of initiatory caspase-9 and downstream caspases-3/7. The fluorescence-quenching studies indicated that PRF extract has binding affinity to human serum albumin, which is one of the factors determining drug bioavailability. Taken together, despite the cytoprotective activity against generated intracellular oxidative stress, V. opulus revealed potential toxic effects as well as decreased insulin secretion from MIN6 cells. These findings are relevant in understanding V. opulus limitations in developing diet supplements designed for the prevention and treatment of postprandial glucose elevation.

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“…They also suggested that these flavonoids change membrane fluidity. Ionescu et al prepared SUVs with DMPC plus 20 or 33 mol% cholesterol to form the L o phase and examine the membrane effect of quercetin (87). FP measurements showed that quercetin increases the fluidity of raft model membranes at 2-16 μM.…”

Section: Flavonoidsmentioning

confidence: 99%

Interaction of drugs with lipid raft membrane domains as a possible target

Tsuchiya

Mizogami

2020

dti

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Introduction: Plasma membranes are not the homogeneous bilayers of uniformly distributed lipids but the lipid complex with laterally separated lipid raft membrane domains, which provide receptor, ion channel and enzyme proteins with a platform. The aim of this article is to review the mechanistic interaction of drugs with membrane lipid rafts and address the question whether drugs induce physicochemical changes in raft-constituting and raft-surrounding membranes. Methods: Literature searches of PubMed/MEDLINE and Google Scholar databases from 2000 to 2020 were conducted to include articles published in English in internationally recognized journals. Collected articles were independently reviewed by title, abstract and text for relevance. Results: The literature search indicated that pharmacologically diverse drugs interact with raft model membranes and cellular membrane lipid rafts. They could physicochemically modify functional protein-localizing membrane lipid rafts and the membranes surrounding such domains, affecting the raft organizational integrity with the resultant exhibition of pharmacological activity. Raft-acting drugs were characterized as ones to decrease membrane fluidity, induce liquid-ordered phase or order plasma membranes, leading to lipid raft formation; and ones to increase membrane fluidity, induce liquid-disordered phase or reduce phase transition temperature, leading to lipid raft disruption. Conclusion: Targeting lipid raft membrane domains would open a new way for drug design and development. Since angiotensin-converting enzyme 2 receptors which are a cell-specific target of and responsible for the cellular entry of novel coronavirus are localized in lipid rafts, agents that specifically disrupt the relevant rafts may be a drug against coronavirus disease 2019.

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Quercetin and epigallocatechin-3-gallate effect on the anisotropy of model membranes with cholesterol

Cited by 20 publications

References 44 publications

Does Green Tea Induce Hormesis?

Does Green Tea Induce Hormesis?

Evaluation of Viburnum opulus L. Fruit Phenolics Cytoprotective Potential on Insulinoma MIN6 Cells Relevant for Diabetes Mellitus and Obesity

Interaction of drugs with lipid raft membrane domains as a possible target

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