Quercetin Attenuates Vascular Calcification through Suppressed Oxidative Stress in Adenine-Induced Chronic Renal Failure Rats

Abstract

Background This study investigated whether quercetin could alleviate vascular calcification in experimental chronic renal failure rats induced by adenine. Methods 32 adult male Wistar rats were randomly divided into 4 groups fed normal diet, normal diet with quercetin supplementation (25 mg/kg·BW/d), 0.75% adenine diet, or adenine diet with quercetin supplementation. All rats were sacrificed after 6 weeks of intervention. Serum renal functions biomarkers and oxidative stress biomarkers were measured and status of vascular calcification in aorta was assessed. Furthermore, the induced nitric oxide synthase (iNOS)/p38 mitogen activated protein kinase (p38MAPK) pathway was determined to explore the potential mechanism. Results Adenine successfully induced renal failure and vascular calcification in rat model. Quercetin supplementation reversed unfavorable changes of phosphorous, uric acid (UA) and creatinine levels, malonaldehyde (MDA) content, and superoxide dismutase (SOD) activity in serum and the increases of calcium and alkaline phosphatase (ALP) activity in the aorta (P < 0.05) and attenuated calcification and calcium accumulation in the medial layer of vasculature in histopathology. Western blot analysis showed that iNOS/p38MAPK pathway was normalized by the quercetin supplementation. Conclusions Quercetin exerted a protective effect on vascular calcification in adenine-induced chronic renal failure rats, possibly through the modulation of oxidative stress and iNOs/p38MAPK pathway.

Perindopril Improves Cardiac Function by Enhancing the Expression of SIRT3 and PGC-1α in a Rat Model of Isoproterenol-Induced Cardiomyopathy

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Novel treatment strategies for chronic kidney disease: insights from the animal kingdom

“…Since a causal role for senescent cells in ageing-related bone loss has been demonstrated in mice (269) nutritional compounds with senolytic effects , such as quercetin and fisetin (270) -found in fruits, capers, vegetables and berries -may also contribute to the capacity of bears to maintain their bone mass (242). Quercetin also blocks phosphate-induced apoptosis and VSMC calcification via inhibition of mitochondrial fission and oxidative stress (271). As polyphenols that stimulate sirtuins and PGC1α prevent muscle wasting induced by mechanical unloading (272) or in streptozotocin-induced diabetes in rats (273), their long-term effects should be tested in patients with CKD who experience muscle wasting.…”

Tongmai Yangxin Pills Improves Cardiac Function by Enhancing the Expression of SIRT3 and PGC-1α in a Rat Model of Isoproterenol-Induced Cardiomyopathy

“…Our data also show that administration of TMYXP 1.0 g/mg, TMYXP 2.0 g/mg and bisoprolol to ISO-induced rats can signi cantly reduce ROS content, increase the activity of Mn-SOD and GSH-Px, and MDA content in myocardial tissue. SODs is an antioxidant enzyme that can eliminate superoxide anion free radicals and protect cells from oxidative damage (19) while Mn-SOD located in mitochondria mainly acts to remove ROS in mitochondria (20). In addition, GSH-Px has the function of eliminating lipid peroxides and forms an antioxidant defense system in the body with other oxidases (21).…”