Quercetin decrease somatic cells count in mastitis of dairy cows

Burmańczuk, Artur; Hola, Piotr; Milczak, A.; Piech, Tomasz; Kowalski, C.; Wojciechowska, Beata; Grabowski, Tomasz

doi:10.1016/j.rvsc.2018.01.006

Cited by 20 publications

(15 citation statements)

References 23 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Lower SCC in milk indicates a better health condition of dairy cows and better milk quality [37]. The result that PM could lower the milk SCC is consistent with prior research [16], which could be the effect of flavonoids in the PM [4], since flavonoids can reduce the milk SCC [38] by enhancing the immunity of dairy cows [39].…”

Section: Dmi Milk Production and Apparent Digestibilitysupporting

confidence: 84%

Effects of Paper Mulberry Silage on the Milk Production, Apparent Digestibility, Antioxidant Capacity, and Fecal Bacteria Composition in Holstein Dairy Cows

Hao¹,

Huang²,

Si³

et al. 2020

Animals

View full text Add to dashboard Cite

Paper mulberry (Broussonetia papyrifera; PM) is an excellent and extensive type of roughage in Asia. This study aimed to evaluate the effects of PM silage on the milk production, apparent digestibility, antioxidant capacity, and fecal bacteria composition in Holstein dairy cows. Forty-five lactating Holstein dairy cows with a similar milk yield and parity were selected and randomly assigned to three groups. The control group was fed a non-PM silage diet, and the PM-treated groups were fed 4.5 and 9.0% PM silage supplementary diets for 28 days. Then, treatment groups were fed diets containing 13.5 and 18.0% PM silage for the next 28 days, respectively. PM silage increased the milk urea nitrogen and decreased the somatic cell count (p < 0.05), but did not affect the dry matter intake, milk yield, apparent digestibility, and energy balance of dairy cows. PM silage can enhance the blood total antioxidant capacity, superoxide dismutase, and immune globulin content (p < 0.05). The PM silage significantly decreased the relative abundance of the genera Ruminococcaceae UCG-013 and Tyzzerella-4 (p < 0.05). In conclusion, PM silage enhanced the antioxidant capacity and immunity of dairy cows, but did not influence the milk yield, dry matter digestibility, and fecal bacteria composition.

show abstract

Section: Dmi Milk Production and Apparent Digestibilitysupporting

confidence: 84%

Effects of Paper Mulberry Silage on the Milk Production, Apparent Digestibility, Antioxidant Capacity, and Fecal Bacteria Composition in Holstein Dairy Cows

Hao¹,

Huang²,

Si³

et al. 2020

Animals

View full text Add to dashboard Cite

show abstract

“…As we know, bacterial biofilm is closely related to its virulence Wroe et al (2020) , and 0.8 mg/ml rutin can interfere with the formation of biofilm, thus effectively reducing the damage of breast tissue caused by S. xylosus infection and protecting mice from mastitis. At the same time, the production of inflammation will cause immune response, release a variety of inflammatory factors including IL-6 and TNF - α, and cause damage to the body ( Kanangat et al, 2001 ; Burmanczuk et al, 2018 ). Therefore, inhibiting the production of IL-6 and TNF - α will prevent inflammation at the same time.…”

Section: Resultsmentioning

confidence: 99%

Rutin, A Natural Inhibitor of IGPD Protein, Partially Inhibits Biofilm Formation in Staphylococcus xylosus ATCC700404 in vitro and in vivo

Cui

Xing

et al. 2021

Front. Pharmacol.

View full text Add to dashboard Cite

Staphylococcus xylosus (S. xylosus) has become an emerging opportunistic pathogen due to its strong biofilm formation ability. Simultaneously, the biofilm of bacteria plays an important role in antibiotic resistance and chronic infection. Here, we confirmed that rutin can effectively inhibit biofilm formation in S. xylosus, of which the inhibition mechanism involves its ability to interact with imidazole glycerol phosphate dehydratase (IGPD), a key enzyme in the process of biofilm formation. We designed experiments to target IGPD and inhibited its activities against S. xylosus. Our results indicated that the activity of IGPD and the amount of histidine decreased significantly under the condition of 0.8 mg/ml rutin. Moreover, the expression of IGPD mRNA (hisB) and IGPD protein was significantly down-regulated. Meanwhile, the results from molecular dynamic simulation and Bio-layer interferometry (BLI) technique showed that rutin could bind to IGPD strongly. Additionally, in vivo studies demonstrated that rutin treatment reduced inflammation and protect mice from acute mastitis caused by S. xylosus. In summary, our findings provide new insights into the treatment of biofilm mediated persistent infections and chronic bacterial infections. It could be helpful to design next generation antibiotics to against resistant bacteria.

show abstract

“…In agreement with this data, we showed that SCC was significantly decreased by AOB along with the number of pathogens such as Staphylococcus. Previous studies have suggested that flavonoids decreased SCCs in milk from dairy cows (4,53). However, investigations of the use of flavonoids extracted from the bamboo leaf in dairy cows are limited.…”

Section: Discussionmentioning

confidence: 99%

“…Quercetin is a flavonoid that affects angiogenesis and vascular inflammation in ruminants ( 37 ). Its anti-inflammatory mechanism was studied by administering multiple intramammary treatments to dairy cows with clinical mastitis resulting in a decrease in SCC ( 53 ). In the present study, quercetin was decreased by AOB supplementation, yet a significant positive correlation was found between quercetin and the presence of Corynebacterium_1, Aerococcus , and Staphylococcus .…”

Section: Discussionmentioning

confidence: 99%

Microbiome and Metabolic Changes of Milk in Response to Dietary Supplementation With Bamboo Leaf Extract in Dairy Cows

Jing-Wei

Yi-Yuan

et al. 2021

Front. Nutr.

View full text Add to dashboard Cite

Bamboo leaf extracts, with high content of flavonoids and diverse biological activities, are used in animal husbandry. Increasing evidence has suggested an association between the bovine physiology and the udder microbiome, yet whether the microbiota and the metabolites of milk affect the mammary gland health or the milk quality remains unknown. In this study, we provide a potential mechanism for the effects of bamboo leaf extracts on milk microbiota and metabolites of dairy cows. Twelve multiparous lactating Chinese Holstein dairy cows were randomly separated into two groups: basal diet as the control group (CON, n = 6) and a diet supplemented with 30 g/d bamboo leaf extract per head as antioxidants of bamboo leaf (AOB) group (AOB, n = 6) for 7 weeks (2-week adaptation, 5-week treatment). Milk samples were collected at the end of the trial (week 7) for microbiome and associated metabolic analysis by 16S ribosomal RNA (rRNA) gene sequencing and liquid chromatography-mass spectrometry (LC-MS). The results showed that the milk protein was increased (p < 0.0001) and somatic cell count (SCC) showed a tendency to decrease (p = 0.09) with AOB supplementation. The relative abundance of Firmicutes was significantly decreased (p = 0.04) while a higher relative abundance of Probacteria (p = 0.01) was seen in the group receiving AOB compared to the CON group. The AOB group had a significantly lower relative abundance of Corynebacterium_1 (p = 0.01), Aerococcus (p = 0.01), and Staphylococcus (p = 0.02). There were 64 different types of metabolites significantly upregulated, namely, glycerophospholipids and fatty acyls, and 15 significantly downregulated metabolites, such as moracetin, sphinganine, and lactulose in the AOB group. Metabolic pathway analysis of the different metabolites revealed that the sphingolipid signaling pathway was significantly enriched, together with glycerophospholipid metabolism, sphingolipid metabolism, and necroptosis in response to AOB supplementation. Several typical metabolites were highly correlated with specific ruminal bacteria, demonstrating a functional correlation between the milk microbiome and the associated metabolites. These insights into the complex mechanism and corresponding biological responses highlight the potential function of AOB, warranting further investigation into the regulatory role of specific pathways in the metabolism.

show abstract

Quercetin decrease somatic cells count in mastitis of dairy cows

Cited by 20 publications

References 23 publications

Effects of Paper Mulberry Silage on the Milk Production, Apparent Digestibility, Antioxidant Capacity, and Fecal Bacteria Composition in Holstein Dairy Cows

Effects of Paper Mulberry Silage on the Milk Production, Apparent Digestibility, Antioxidant Capacity, and Fecal Bacteria Composition in Holstein Dairy Cows

Rutin, A Natural Inhibitor of IGPD Protein, Partially Inhibits Biofilm Formation in Staphylococcus xylosus ATCC700404 in vitro and in vivo

Microbiome and Metabolic Changes of Milk in Response to Dietary Supplementation With Bamboo Leaf Extract in Dairy Cows

Contact Info

Product

Resources

About