Quercetin downregulates matrix metalloproteinases 2 and 9 proteins expression in prostate cancer cells (PC-3)

Vijayababu, M.R.; Arumugam, Arunkumar; Palaniyandi, Kanagaraj; Venkataraman, P.; Giribabu, K.; Arunakaran, J.

doi:10.1007/s11010-005-9085-3

Cited by 159 publications

(95 citation statements)

References 28 publications

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“…Thus, EGCG seemed to prevent metastasis and invasion, but it is worth mentioning that EGCG unexpectedly induced proMMP-7 induction and production in HT-29 human colorectal cancer cells through ROS formation and activation of JNK1/2, c-JUN, c-FOS and AP-1, but not p38 MAPK or ERK1/2 [106]. On the other hand, quercetin and luteolin were able to inactivate the EGFR tyrosine kinase activity, which has been proposed to stimulate cell migration and downstream signalling pathways and to reduce FAK phosphorylation and MMP secretion (MMP-2 and -9) by unclear mechanisms at present [124,161,162]. Down-regulation of MMP-1 and -2 has also been reported by curcumin due to the inhibition of NFjB/AP-1 mediated transcription which can explain the reduced invasion in vitro and metastasis in vivo [163].…”

Section: Inhibition Of Metastasismentioning

confidence: 92%

Cancer chemoprevention and chemotherapy: Dietary polyphenols and signalling pathways

Ramos¹

2008

Molecular Nutrition Food Res

627

499

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Prevention of cancer through dietary intervention recently has received an increasing interest, and dietary polyphenols have become not only important potential chemopreventive, but also therapeutic, natural agents. Polyphenols have been reported to interfere at the initiation, promotion and progression of cancer. They might lead to the modulation of proteins in diverse pathways and require the integration of different signals for the final chemopreventive or therapeutic effect. Polyphenols have been demonstrated to act on multiple key elements in signal transduction pathways related to cellular proliferation, differentiation, apoptosis, inflammation, angiogenesis and metastasis; however, these molecular mechanisms of action are not completely characterized and many features remain to be elucidated. The aim of this review is to provide insights into the molecular basis of potential chemopreventive and therapeutic activities of dietary polyphenols with emphasis in their ability to control intracellular signalling cascades considered as relevant targets in a cancer preventive approach.

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Section: Inhibition Of Metastasismentioning

confidence: 92%

Cancer chemoprevention and chemotherapy: Dietary polyphenols and signalling pathways

Ramos¹

2008

Molecular Nutrition Food Res

627

499

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“…Matrix metalloproteinases are expressed by many invasive cancer cells and are the key proteins involved in cell invasion and metastasis (Stetler-Stevenson, 2001; Deryugina and Quigley, 2006). PC-3 cells are among the cells that express high levels of matrix metalloproteinases including MMP-2 and MMP-9 (Vijayababu et al, 2006) and therefore, gelatin zymography is an appropriate assay to measure the invasion ability of these cells (see the Methods). PC-3 cells were treated with several concentrations of T persicum extract for 48 hours and then the media of these cells were examined for the presence of matrix metalloproteinases as described in the Methods.…”

Section: T Persicum Inhibits Invasion Of Pc-3 Cellsmentioning

confidence: 99%

Anticancer Properties of Teucrium persicum in PC-3 Prostate Cancer Cells

Tafrihi

Toosi²,

Minaei³

et al. 2014

Asian Pacific Journal of Cancer Prevention

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Crude extracts or phytochemicals obtained from some plants have potential anti-cancer properties. Teucrium persicum is an Iranian endemic plant belonging to the Lamiaceae family which has traditionally been used to relieve abdominal pains. However, the anti-cancer properties of this species of the Teucrium genus have not been investigated previously. In this study, we have used a highly invasive prostate cancer cell line, PC-3, which is an appropriate cell system to study anti-tumor properties of plants. A methanolic extract obtained from T persicum potently inhibited viability of PC-3 cells. The viability of SW480 colon and T47D breast cancer cells was also significantly decreased in the presence of the T persicum extract. Flow cytometry suggested that the reduction of cell viability was due to induction of apoptosis. In addition, the results of wound healing and gelatin zymography experiments supported anti-cell invasion activity of T persicum. Interestingly, sublethal concentrations of T persicum extract induced an epithelial-like morphology in a subpopulation of cells with an increase in E-Cadherin and β-Catenin protein levels at the cell membrane. These results strongly suggest that T persicum is a plant with very potent anti-tumor activity

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“…Quercetin, which is known to inhibit the activity of MMP-2 and MMP-9 in cells (Vijayababu et al, 2006) was also used as an additional control. The gelatin zymography studies (Fig.…”

Section: Regulation Of Mmp-2 Activity By Components Of Cnsementioning

confidence: 99%

Anacardic Acid Inhibits the Catalytic Activity of Matrix Metalloproteinase-2 and Matrix Metalloproteinase-9

Omanakuttan

Nambiar

Harris³

et al. 2012

Mol Pharmacol

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Cashew nut shell liquid (CNSL) has been used in traditional medicine for the treatment of a wide variety of pathophysiological conditions. To further define the mechanism of CNSL action, we investigated the effect of cashew nut shell extract (CNSE) on two matrix metalloproteinases, MMP-2/gelatinase A and MMP-9/gelatinase B, which are known to have critical roles in several disease states. We observed that the major constituent of CNSE, anacardic acid, markedly inhibited the gelatinase activity of 3T3-L1 cells. Our gelatin zymography studies on these two secreted gelatinases, present in the conditioned media from 3T3-L1 cells, established that anacardic acid directly inhibited the catalytic activities of both MMP-2 and MMP-9. Our docking studies suggested that anacardic acid binds into the MMP-2/9 active site, with the carboxylate group of anacardic acid chelating the catalytic zinc ion and forming a hydrogen bond to a key catalytic glutamate side chain and the C15 aliphatic group being accommodated within the relatively large S1Ј pocket of these gelatinases. In agreement with the docking results, our fluorescence-based studies on the recombinant MMP-2 catalytic core domain demonstrated that anacardic acid directly inhibits substrate peptide cleavage in a dosedependent manner, with an IC 50 of 11.11 M. In addition, our gelatinase zymography and fluorescence data confirmed that the cardol-cardanol mixture, salicylic acid, and aspirin, all of which lack key functional groups present in anacardic acid, are much weaker MMP-2/MMP-9 inhibitors. Our results provide the first evidence for inhibition of gelatinase catalytic activity by anacardic acid, providing a novel template for drug discovery and a molecular mechanism potentially involved in CNSL therapeutic action.

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Quercetin downregulates matrix metalloproteinases 2 and 9 proteins expression in prostate cancer cells (PC-3)

Abstract: Hence, we speculated that inhibition of metastasis-specific MMPs in cancer cells may be one of the targets for anticancer function of quercetin, and thus provides the molecular basis for the development of quercetin as a novel chemopreventive agent for metastatic prostate cancer.

Cited by 159 publications

References 28 publications

Cancer chemoprevention and chemotherapy: Dietary polyphenols and signalling pathways

Cancer chemoprevention and chemotherapy: Dietary polyphenols and signalling pathways

Anticancer Properties of Teucrium persicum in PC-3 Prostate Cancer Cells

Anacardic Acid Inhibits the Catalytic Activity of Matrix Metalloproteinase-2 and Matrix Metalloproteinase-9

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