Quercetin exerts a neuroprotective effect through inhibition of the iNOS/NO system and pro-inflammation gene expression in PC12 cells and in zebrafish

Lee, Myeong Soo

doi:10.3892/ijmm.2010.571

Cited by 76 publications

(42 citation statements)

References 46 publications

(50 reference statements)

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“…QUE can attenuate liver injury [56] and renal impairment [57], [58], and provide neuroprotective protection against excitotoxic insults [59], [60]. KAE can also provide cardio- and neuro-protections [61].…”

Section: Discussionmentioning

confidence: 99%

Alleviation of Podophyllotoxin Toxicity Using Coexisting Flavonoids from Dysosma versipellis

Sun

Jin

et al. 2013

PLoS ONE

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Podophyllotoxin (POD) is a lignan-type toxin existing in many herbs used in folk medicine. Until now, no effective strategy is available for the management of POD intoxication. This study aims to determine the protective effects of ﬂavonoids (quercetin and kaempferol) on POD-induced toxicity. In Vero cells, both ﬂavonoids protected POD-induced cytotoxicity by recovering alleviating G2/M arrest, decreasing ROS generation and changes of membrane potential, and recovering microtubule structure. In Swiss mice, the group given both POD and ﬂavonoids group had significantly lower mortality rate and showed less damages in the liver and kidney than the group given POD alone. As compared to the POD group, the POD plus ﬂavonoids group exhibited decreases in plasma transaminases, alkaline phosphatase, lactate dehydrogenase, plasma urea, creatinine and malondialdehyde levels, and increases in superoxide dismutase and glutathione levels. Histological examination of the liver and kidney showed less pathological changes in the treatment of POD plus ﬂavonoids group. The protective mechanisms were due to the antioxidant activity of ﬂavonoids against the oxidative stress induced by POD and the competitive binding of ﬂavonoids against POD for the same colchicines-binding sites. The latter binding was confirmed by the tubulin assembly assay in combination with molecular docking analyses. In conclusion, this study for the first time demonstrated that the coexisting flavonoids have great protective effects against the POD toxicity, and results of this study highlighted the great potential of searching for effective antidotes against toxins based on the pharmacological clues.

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Section: Discussionmentioning

confidence: 99%

Alleviation of Podophyllotoxin Toxicity Using Coexisting Flavonoids from Dysosma versipellis

Sun

Jin

et al. 2013

PLoS ONE

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“…Wild-type zebrafish embryos (1 days post fertilization (dpf)) were treated with pure compounds or fractionsin zebrafish model for 2 days. After treatment, anti-tyrosine hydroxylase (TH) whole-mount immunostaining was performed using literature reported method5758. Zebrafish were fixed in 4% paraformaldehyde in PBS for 5 h, rinsed, and stored at −20 °C in 100% ethanol.…”

Section: Methodsmentioning

confidence: 99%

Chemical profiling analysis of Maca using UHPLC-ESI-Orbitrap MS coupled with UHPLC-ESI-QqQ MS and the neuroprotective study on its active ingredients

Zhou

Li²,

Brantner

et al. 2017

Sci Rep

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Lepidium meyenii (Maca), originated from Peru, has been cultivated widely in China as a popular health care food. However, the chemical and effective studies of Maca were less in-depth, which restricted its application seriously. To ensure the quality of Maca, a feasible and accurate strategy was established. One hundred and sixty compounds including 30 reference standards were identified in 6 fractions of methanol extract of Maca by UHPLC-ESI-Orbitrap MS. Among them, 15 representative active compounds were simultaneously determined in 17 samples by UHPLC-ESI-QqQ MS. The results suggested that Maca from Yunnan province was the potential substitute for the one from Peru. Meanwhile, the neuroprotective effects of Maca were investigated. Three fractions and two pure compounds showed strong activities in the 1-methyl-4-phenyl-1, 2, 3, 6-tetrahydropyridine (MPTP)-induced zebrafish model. Among them, 80% methanol elution fraction (Fr5) showed significant neuroprotective activity, followed by 100% part (Fr6). The inhibition of acetylcholinesterase (AChE) and butyrylcholinesterase (BuChE) was a possible mechanism of its neuroprotective effect.

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“…In vitro studies have shown that quercetin antagonizes cell toxicity induced by various oxidants and other neurotoxic molecules believed to act by inducing oxidative stress [11–15]. In addition, several studies show that quercetin can exert neuroprotection and antagonize oxidative stress when administered in vivo .…”

Section: Introductionmentioning

confidence: 99%

Modulation of Paraoxonase 2 (PON2) in Mouse Brain by the Polyphenol Quercetin: A Mechanism of Neuroprotection?

et al. 2013

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Quercetin is a common flavonoid polyphenol which has been shown to exert neuroprotective actions in vitro and in vivo. Though quercetin has antioxidant properties, it has been suggested that neuroprotection may be ascribed to its ability of inducing the cell’s own defense mechanisms. The present study investigated whether quercetin could increase the levels of paraoxonase 2 (PON2), a mitochondrial enzyme expressed in brain cells, which has been shown to have potent antioxidant properties. PON2 protein, mRNA, and lactonase activity were highest in mouse striatal astrocytes. Quercetin increased PON2 levels, possibly by activating the JNK/AP-1 pathway. The increased PON2 levels induced by quercetin resulted in decreased oxidative stress and ensuing toxicity induced by two oxidants. The neuroprotective effect of quercetin was significantly diminished in cells from PON2 knockout mice. These findings suggest that induction of PON2 by quercetin represents an important mechanism by which this polyphenol may exert its neuroprotective action.

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Quercetin exerts a neuroprotective effect through inhibition of the iNOS/NO system and pro-inflammation gene expression in PC12 cells and in zebrafish

Cited by 76 publications

References 46 publications

Alleviation of Podophyllotoxin Toxicity Using Coexisting Flavonoids from Dysosma versipellis

Alleviation of Podophyllotoxin Toxicity Using Coexisting Flavonoids from Dysosma versipellis

Chemical profiling analysis of Maca using UHPLC-ESI-Orbitrap MS coupled with UHPLC-ESI-QqQ MS and the neuroprotective study on its active ingredients

Modulation of Paraoxonase 2 (PON2) in Mouse Brain by the Polyphenol Quercetin: A Mechanism of Neuroprotection?

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