Quercetin induces apoptosis and cell cycle arrest in triple-negative breast cancer cells through modulation of Foxo3a activity

Nguyen, Loan T. T.; Lee, Yeon-Hee; Sharma, Ashish Ranjan; Park, Jong-Bong; Jagga, Supriya; Sharma, Garima; Lee, Sang-Soo; Nam, Ju-Suk

doi:10.4196/kjpp.2017.21.2.205

Cited by 139 publications

(100 citation statements)

References 49 publications

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“…The antitumor properties of flavonoids are discussed in the literature [21][22][23]. The data obtained by us are consistent with the results of other authors demonstrating that flavonoids activate apoptosis in tumor cells [24,25], while not having toxicity. Thus, it is known that the flavonoid Vogonin as part of the popular Chinese herbal remedy Huang-Qin (based on grass skullcap Baikal) induces apoptosis in a wide range of human tumor cells in vitro and inhibits tumor growth in vivo in various models of mouse tumors [3,[29][30][31][32][33][34].…”

Section: Discussionsupporting

confidence: 90%

The apoptotic activity of flavonoid-containing Gratiola officinalis extract in cell cultures of human kidney cancer

Polukonova¹,

Navolokin²,

Bucharskaya³

et al. 2018

Russ Open Med J

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Objective -The discovery of the apoptosis-inducing effects of flavonoid vagonin allowed to make an assumption of existence of similar effect in others flavonoids. This study is devoted to the effects of Gratīola officinālis extract on cell culture of the human kidney cancer.Methods -Cell cultures of human kidney carcinoma -Caki-1 and SN12c were used in the study. The cells were stained with Hoechat 33258 dye. The number of living cells, cells in a state of apoptosis and mitosis were counted. The Cramer-Welch criterion (T) was used to compare the obtained data.Results -The activation of apoptosis was noted at all concentrations of the Gratiola officinalis L. extract during the first day of exposure. The apoptotic activity increased with increasing of extract concentration. After 48 hours, this activity was maintained only at a Gratiola officinalis L. extract concentration of 0.9 mg/ml. After 24 hours, the apoptotic activity of the extract was more expressed in the culture of CaKi-1. However, after 48 hours the extract induces more pronounced apoptosis in the culture of Sn12c cells. The cytotoxic activity of the extract was not differ after 24 hours in both cultures, after 48 hours it was more pronounced in the culture of CaKi-1. Conclusion -We revealed a pronounced antitumor and apoptotic activity of the Gratiola officinalis L. extract against the cultures of the kidney cancer Caki-1 and Sn12c. Apoptosis of tumor cells can be manifested in the form of pycnosis of the nucleus, the formation of apoptotic bodies and the emergence of cellular debris resulting from complete degradation of tumor cells. The apoptotic activity of the Gratiola officinalis extract depends on their concentration.

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Section: Discussionsupporting

confidence: 90%

The apoptotic activity of flavonoid-containing Gratiola officinalis extract in cell cultures of human kidney cancer

Polukonova¹,

Navolokin²,

Bucharskaya³

et al. 2018

Russ Open Med J

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“…Contradictory results on the cell population percentage altered with quercetin were also reported in these studies, where higher G2/M cell population after quercetin administration was reported [22,30], while two different investigations found a decrease in this phase of the cell cycle [39,49]. Similarly, one study determined that quercetin-derived cell cycle detention was in S and G2/M phases in MDA-MB-231 breast cancer line [66] in contrast to the G0/G1 phase arrest observed in MCF-7 breast cancer cell line [65]. This variable role of quercetin on cell cycle regulation has also been shown in cells models of non-small cell lung cancer [64] and breast cancer [65,66], and enhanced through its combination [68], encapsulation [69], as well as with quercetin derivatives [71].…”

Section: Discussionmentioning

confidence: 56%

“…Results presented in this article collect a great variety of antitumor actions of either quercetin or the mentioned strategies of combination, encapsulation and derived compounds. Cell growth inhibition, in addition to apoptosis stimulation, were the main processes described as quercetin mechanisms of action against HCC, properties that have also been observed in different tumors, such as non-small cell lung cancer [64] and breast cancer [65,66]. Although almost studies associated antiproliferative activity of quercetin with alteration of several pathways, Akt/mTOR and MEK1/ERK1/2 signaling routes were mostly found to be regulated by this flavonoid, either as free drug as well as encapsulated [23,24,27,38,42].…”

Section: Discussionmentioning

confidence: 96%

“…Similarly, one study determined that quercetin-derived cell cycle detention was in S and G2/M phases in MDA-MB-231 breast cancer line [66] in contrast to the G0/G1 phase arrest observed in MCF-7 breast cancer cell line [65]. This variable role of quercetin on cell cycle regulation has also been shown in cells models of non-small cell lung cancer [64] and breast cancer [65,66], and enhanced through its combination [68], encapsulation [69], as well as with quercetin derivatives [71]. Even though quercetin was found to arrest cell cycle progression in several studies included in this systematic review, results were contradictory as opposite effects in cell population percentage were reported.…”

Section: Discussionmentioning

confidence: 97%

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Antitumor Effects of Quercetin in Hepatocarcinoma In Vitro and In Vivo Models: A Systematic Review

et al. 2019

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Quercetin is a flavonoid present in fruits, vegetables and plants with antioxidant, anti-inflammatory and anticancer properties. Its beneficial activities have been demonstrated in different human pathologies, including hepatoprotective effects against liver disorders. High mortality and late diagnosis of the primary liver tumor hepatocarcinoma (HCC) makes this cancer an interesting target for the study of quercetin effects. Our aim was to systematically review antitumor activities of quercetin in HCC preclinical studies employing single, encapsulated, combined or derived quercetin forms. Literature search was conducted in PubMed, Scopus and Web of Science (WOS), and 39 studies were finally included. We found that 17 articles evaluated quercetin effects alone, six used encapsulated strategy, 10 combined this flavonoid, two decided to co-encapsulate it and only four studied effects of quercetin derivatives, highlighting that only nine included in vivo models. Results evidence the quercetin antiproliferative and proapoptotic properties against HCC either alone and with the mentioned strategies; nevertheless, few investigations assessed specific activities on different processes related with cancer progression. Overall, further studies including animal models are needed to deeper investigate the precise mechanisms of action of quercetin as antitumor agent, as well as the potential of novel strategies aimed to improve quercetin effects in HCC.

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“…Although Triple negative breast cancer accounts for only 15% of other breast cancer subtypes, it shows high rate of recurrence and develops resistance to chemotherapy as previously reported by (Rakha et al, 2008). In addition, patients have shown poor prognosis due to the lack of specific targeted therapy (Nguyen et al, 2017). Therefore, it is vital to look for new therapeutic agents that are effective, less toxic and can prevent relapse emergence.…”

Section: Introductionmentioning

confidence: 94%

Synthesis, Ant Proliferative Activity and Docking Study of New Quercetin Derivatives against MDA-MB231 Breast Cancer Cell Lines

Al-Anssari

Elias

Al-Jadaan

2019

American Journal of Applied Sciences

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MDA-MB231 is a very aggressive and invasive triple negative breast cancer, which present with limited treatment options. Unlike other breast cancer types, it is characterized by absence of hormonal receptors of estrogen, progesterone as well as human epidermal growth factor receptor 2, rendering it unsuitable for hormonal therapy and a perfect candidate for chemotherapy. Quercetin is a common natural flavonoid present in many food items, which have a wide range of biological activities, likes anticancer, antiviral, antibacterial and antioxidant. This study involves the synthesis of new Quercetin derivatives and the investigation their effects against MDA-MB231 cell lines. The structures of the derivatives were established using UV, IR, 1 HNMR, CHNS, EIMS and ESIMS techniques. Their antiproliferative activities were investigated in vitro using Microculture Tetrazolium (MTT) assay. The percentage cell viability following exposure to Quercetin and its derivatives (Compounds 1-5) were measured. Both compounds 1 and 4 show a significant decrease in percentage cell viability from 100% to 43.7% and 38.1% respectively. IC 50 value was calculated for compound 1 and 4 and found to be 2.042 and 1.838 µM respectively, indicating that they have a potential anticancer activity against the triple negative breast cancer type. The antiproliferative activity was supported and evidenced by molecular docking study.

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Quercetin induces apoptosis and cell cycle arrest in triple-negative breast cancer cells through modulation of Foxo3a activity

Cited by 139 publications

References 49 publications

The apoptotic activity of flavonoid-containing Gratiola officinalis extract in cell cultures of human kidney cancer

The apoptotic activity of flavonoid-containing Gratiola officinalis extract in cell cultures of human kidney cancer

Antitumor Effects of Quercetin in Hepatocarcinoma In Vitro and In Vivo Models: A Systematic Review

Synthesis, Ant Proliferative Activity and Docking Study of New Quercetin Derivatives against MDA-MB231 Breast Cancer Cell Lines

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