Quercetin Inhibits Inflammatory Bone Resorption in a Mouse Periodontitis Model

Abstract

Periodontitis is a disease that leads to bone destruction and represents the main cause of tooth loss in adults. The development of aggressive periodontitis has been associated with increased inflammatory response that is induced by the presence of a subgingival biofilm containing Aggregatibacter actinomycetemcomitans. The flavonoid quercetin (1) is widespread in vegetables and fruits and exhibits many biological properties for possible medical and clinical applications such as its anti-inflamatory and antioxidant effects. Thus, in the present study, the properties of 1 have been evaluated in bone loss and inflammation using a mouse periodontitis model induced by A. actinomycetemcomitans infection. Subcutaneous treatment with 1 reduced A. actinomycetemcomitans-induced bone loss and IL-1β, TNF-α, IL-17, RANKL, and ICAM-1 production in the gingival tissue without affecting bacterial counts. These results demonstrated that quercetin exhibits protective effects in A. actinomycetemcomitans-induced periodontitis in mice by modulating cytokine and ICAM-1 production.

Dietary antioxidants for chronic periodontitis prevention and its treatment: a review on current evidences from animal and human studies

“…Similar effect on alveolar bone has been observed in a mouse periodontitis model induced by A. actinomycetemcomitans infection for the compound 84 . Additionally, when it was measured, it was noted that this effect on bone loss correlates with reduced oxidative stress 87 or levels of enzymes that promote it like iNOS 83,87 .…”

“…Similarly, beneficial effect has been also reported for different polyphenols [80][81][82][83][84]87 , as well as CoQ 99 but they only have been tested in animals, although for many their in vivo antioxidant effects seems due to iNOS expression or activity modulation and not to free radical scavenging. Unfortunately, at the moment, experimental interventions in humans have been reduced to vitamin C 54-57 but also for E 76 or Zinc 78 , where vitamin E have result the most interesting although it were used after mechanical treatment.…”

“…The additon of several specific polyphenols to the diet have been tested in rodents models of periodontitis. In rat models of ligatureinduced periodontitis oral administration of resveratrol (trans-3,4ʹ,-5-trihydroxystilbene) 80,81 , mangiferin (C2-b-dglucopyranosyl-1,3,6,7-tetrahydroxyxanthone) 82 , baicalin (7-glucuronic acid, 5,6-dihydroxy-flavone) 83 , verbascoside (4,5-hydroxyphenylethanol bound to a b-(d)-glucopyranoside) and quercetin (3,5,7,3ʹ,4ʹ-pentahydroxyflavone) 84 have…”

The Effect of Calendula officinalis on Oxidative Stress and Bone Loss in Experimental Periodontitis

“…In vitro , quercetin significantly increased osteoblast differentiation (Zhou et al, 2015) and induced mRNA expression of sialoprotein and osteocalcin in osteoblast culture (Satué et al, 2013). In vivo , quercetin inhibited bone loss in periodontitis models in rats (Napimoga et al, 2013), increased serum osteocalcin and the activity of alkaline phosphatase (Liang et al, 2011), contributing to bone tissue preservation.…”

The Effect of <b><i>Allium cepa</i></b> Extract on Lung Oxidant, Antioxidant, and Immunological Biomarkers in Ovalbumin-Sensitized Rats

“…Results were calculated using the standard curves plotted for each assay. The assays were carried out in a blind fashion in duplicate [16]. The interassay coefficients of variation for IFN-γ, IL-4, and IgE were < 12, 5.9, and < 10%, respectively.…”