Quercetin inhibits porcine intestinal inflammation <i>in vitro</i>

Abstract

Purpose: To investigate the effect of Quercetin (Que) on inflammatory reaction in intestinal porcine enterocyte cells, IPEC-J2, induced by lipopolysaccharide (LPS). Methods: IPEC-J2 cells were pretreated with Que and then incubated with LPS. Cell viability, cell morphology, nitric oxide (NO) content, gene expression levels of interleukin-6 (IL-6) and interleukin-8 (IL-8), were then evaluated. Results: LPS incubation at 10 μg/mL for 24 h showed no effect on the viability and cell morphology of IPEC-J2, while Que pre-incubation significantly enhanced the viability of IPEC-J2 and improved cell morphology (p < 0.05). Que decreased the expression levels of pro-inflammatory cytokines IL-6 mRNA and IL-8 mRNA raised by LPS (p < 0.05). Interestingly, both LPS and Que demonstrated no influence on the release of NO. Conclusion: Pre-treatment of Que shows a positive effect on intestinal porcine enterocyte cells and inhibited porcine intestinal inflammation in vitro induced by LPS, but the mechanism may be not associated with NO-related signaling pathway. Therefore, Que might have a potential effect as a veterinary drug or feed additive for the treatment of enteritis in pigs.

Omega-3 Polyunsaturated Fatty Acids Counteract Inflammatory and Oxidative Damage of Non-Transformed Porcine Enterocytes

“…It was reported that high concentrations of LPS between 100 and 300 µg/mL are required to reduce the viability of non-transformed rat IEC-18 enterocytes after 24 h [ 35 ]. LPS concentrations between 1 and 10 µg/mL did not affect IPEC-J2 viability until 24 h, while 10 µg/mL activated the expression of pro-inflammatory IL6 and IL8 mRNA [ 36 ]. An LPS concentration of 10 µg/mL activated pro-inflammatory genes in 8 h, although it did not cause any cytotoxic effects after 24 h [ 37 ].…”

Differential Effects of Dietary Oregano Essential Oil on the Inflammation Related Gene Expression in Peripheral Blood Mononuclear Cells From Outdoor and Indoor Reared Pigs

“…Among the possible management interventions, the incorporation of plant-derived compounds into the diet appears to be a strategy to improve swine health, performance, and food-derived quality (25,26). Essential oils, from plant derivatives, are used for their antimicrobial, antioxidant, and anti-inflammatory properties (27)(28)(29)(30). Among essential oils, oregano essential oil (ORE) is used in the pig industry for its beneficial effects, ranging from improved performance to increased pork quality (31,32).…”