Quercetin is an effective inhibitor of quorum sensing, biofilm formation and virulence factors in <i>Pseudomonas aeruginosa</i>

Ouyang, Jing; Sun, Fengjun; Feng, Wei; Sun, Yanxin; Qiu, Xuwen; Xiong, Lize; Liu, Y.; Chen, Y.

doi:10.1111/jam.13073

Cited by 227 publications

(160 citation statements)

References 52 publications

(105 reference statements)

Supporting

Mentioning

147

Contrasting

Unclassified

Order By: Relevance

“…Analysis of antibacterial activity. The minimal inhibitory concentrations (MICs) of L12 and various antibiotics were calculated using the broth microdilution method (12) for each type of strain, according to the guidelines of The Clinical and Laboratory Standards Institute (2015) (13). The MIC for each drug was set as the lowest concentration required to inhibit bacterial growth.…”

Section: Methodsmentioning

confidence: 99%

Effects of the antimicrobial peptide L12 against multidrug‑resistant Staphylococcus aureus

Dai

et al. 2019

Mol Med Report

View full text Add to dashboard Cite

Methicillin-resistant Staphylococcus aureus (S. aureus; MRSA) is one of the most common bacterial pathogens and MRSA infections are characterized by high mortality rates. Antimicrobial peptides are considered one of the most promising drugs for the treatment of resistant strains of S. aureus. The present study aimed to examine the antimicrobial activity of L12 against numerous bacterial species using the broth microdilution method. Furthermore, the synergistic effect of L12 combined with various antibacterial drugs was tested, and its antibacterial mechanism was investigated by a checkerboard assay. The alterations in bacterial morphology were detected by electron microscopy, and biofilm formation and removal were tested by crystal violet staining. The present results suggested that L12 affected the growth of gram-positive strains, particularly S. aureus. Electron microscopy analysis suggested that L12 may target the cell membrane, and L12 increased the antibacterial activity of vancomycin and levofloxacin, exerting a synergistic effect. However, the minimal inhibitory concentrations (MICs) of L12 were not correlated with antibiotic resistance, the strains resistant to more antibiotics were not more resistant to L12. A sub-MIC of L12 was able to inhibit biofilm formation in a dose-dependent manner; however, concentrations of L12 ≤10 times the MIC were not sufficient to degrade previously formed biofilm. Collectively, the present study suggested that L12 may represent a novel potential therapeutic molecule for the treatment of S. aureus infections.

show abstract

Section: Methodsmentioning

confidence: 99%

Effects of the antimicrobial peptide L12 against multidrug‑resistant Staphylococcus aureus

Dai

et al. 2019

Mol Med Report

View full text Add to dashboard Cite

show abstract

“…In addition, many other studies have addressed the ability of phenolic compounds and different plant extracts to modulate the quorum sensing system and the biofilm formation in foodborne pathogenic and spoilage bacteria. For instance, catechin [114], naringenin [115] and quercetin [116] depicted strong anti-quorum sensing property against P. aeruginosa. Additionally, flavonoid fraction of guava (Psidium guajava L.) leaves extract inhibited the quorum sensing system of C. violaceum, and the biofilm formation, pyocyanin production, proteolytic and elastolytic activities and swarming motility in P. aeruginosa PAO1 [117].…”

Section: Anti-quorum Sensing and Antibiofilm Effects Of Plant Phenolicsmentioning

confidence: 99%

Plant Phenolics and Phenolic-Enriched Extracts as Antimicrobial Agents against Food-Contaminating Microorganisms

et al. 2020

View full text Add to dashboard Cite

Phenolic compounds and extracts with bioactive properties can be obtained from many kinds of plant materials. These natural substances have gained attention in the food research as possible growth inhibitors of foodborne pathogenic and spoilage bacteria. Many phenolic-enriched plant extracts and individual phenolics have promising anti-quorum sensing potential as well and can suppress the biofilm formation and toxin production of food-related pathogens. Various studies have shown that plant phenolics can substitute or support the activity of synthetic food preservatives and disinfectants, which, by the way, can provoke serious concerns in consumers. In this review, we will provide a brief insight into the bioactive properties, i.e., the antimicrobial, anti-quorum sensing, anti-biofilm and anti-enterotoxin activities, of plant phenolic extracts and compounds, with special attention to pathogen microorganisms that have food relation. Carbohydrase aided applications to improve the antimicrobial properties of phenolic extracts are also discussed.

show abstract

“…Thus, inhibiting the motility may reduce the biofilm formation to a certain extent. The study by Ouyang et al (2016) reported the biofilm inhibition by quercetin against P. aeruginosa PAO1 strain at a concentration of 64 lg ml -1 with 20% inhibition. However, in this study, at 250 lg ml -1 concentration quercetin showed inhibition in the range of 43Á123 to 78Á72% against PAO1 and other clinical strains of P. aeruginosa (Fig.…”

Section: Resultsmentioning

confidence: 99%

“…; Ouyang et al . ). Hence, this study investigated into its activities against key virulence factors of catheter associated P. aeruginosa strains and protective role to the host cells during infection by P. aeruginosa in vitro .…”

Section: Introductionmentioning

confidence: 97%

“…Quercetin is a naturally occurring flavonoid that has a long history of consumption as part of the normal human diet (Cseke et al 2016). It has anti-quorum sensing activity against Gram-negative bacteria (Gopu et al 2015;Vasavi et al 2016) and inhibition of biofilm formation and virulence factors in P. aeruginosa (Vasavi et al 2014;Ouyang et al 2016). Hence, this study investigated into its activities against key virulence factors of catheter associated P. aeruginosa strains and protective role to the host cells during infection by P. aeruginosa in vitro.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Anti‐biofilm and cytoprotective activities of quercetin againstPseudomonas aeruginosaisolates

Vipin

Mujeeburahiman

Ashwini

et al. 2019

Lett Appl Microbiol

View full text Add to dashboard Cite

Increase in infection with multidrug resistant Pseudomonas aeruginosa is a serious global challenge in healthcare. Pseudomonas aeruginosa is capable of causing human infection in various sites and complicates the infection due to its virulence factors. This study was aimed to investigate the effect of quercetin, a dietary flavonoid against the virulence factors of P. aeruginosa and its cell protective effects on epithelial cells. Bactericidal activity, anti‐biofilm activity and effect on different virulence factors were carried out using standard methods by using five P. aeruginosa isolates. Cytotoxicity and cell protective effect of quercetin was evaluated by trypan blue dye exclusion assay. All the tested isolates were completely inhibited (100%) by quercetin at a concentration of 500 μg ml–1. It showed significant (P < 0·05) inhibitory effect on virulence factors including biofilm formation and showed significant protective effect on HEK 293T cells infected with P. aeruginosa strains. This study supports the role of quercetin against P. aeruginosa, by inhibiting virulence factors as well as its cytoprotective activity during bacterial infection either by attenuating the virulence or providing direct protective effect to the host cells. Significance and Impact of the Study The increase in infections caused by opportunistic pathogen Pseudomonas aeruginosa is a serious concern in the health care system. This study describes the beneficial effects of a dietary flavonoid, quercetin against pathogenic P. aeruginosa strains and its protective effect against the P. aeruginosa infection in HEK 293T cells in vitro.

show abstract

Quercetin is an effective inhibitor of quorum sensing, biofilm formation and virulence factors in Pseudomonas aeruginosa

Abstract: This is the first study to demonstrate that quercetin is an effective inhibitor of QS, biofilm formation and virulence factors in Ps. aeruginosa. Furthermore, quercetin might have potential in fighting biofilm-related infections.

Cited by 227 publications

References 52 publications

Effects of the antimicrobial peptide L12 against multidrug‑resistant Staphylococcus aureus

Effects of the antimicrobial peptide L12 against multidrug‑resistant Staphylococcus aureus

Plant Phenolics and Phenolic-Enriched Extracts as Antimicrobial Agents against Food-Contaminating Microorganisms

Anti‐biofilm and cytoprotective activities of quercetin againstPseudomonas aeruginosaisolates

Contact Info

Product

Resources

About